Colon

The colon smell like poo... 'cause that's where poo comes from. This article also covers the rectum and cecum as both have a similar mucosa.

It commonly comes to pathologists because there is a suspicion of colorectal cancer or a known history of inflammatory bowel disease (IBD).

An introduction to gastrointestinal pathology is found in the gastrointestinal pathology article. The anus is dealt with in a separate article.

Technically, the rectum and cecum are not part of the colon. Thus, inflammation of the rectum should be proctitis and inflammation of the cecum should be cecitis.

Common clinical problems

Obstruction

Top three (in adults):

• Neoplasia.
• Volvulus (cecal, sigmoid).
• Diverticular disease + stricture formation.

Bleeding

Mnemonic CHAND:

• Colitis (radiation, infectious, ischemic, IBD (UC >CD), iatrogenic (anticoagulants)).
• Hemorrhoids.
• Angiodysplasia.
• Neoplastic.
• Diverticular disease.

Infectious colitis with bleeding - causes:

• Enterohemorrhagic Escherichia coli (EHEC) -- commonly 0157:H7.
• Campylobacter jejuni.
• Clostridium difficile.
• Shigella.

Infectious colitis in the immunosuppressed:

• Cytomegalovirus (CMV).
  • May afflict patients with IBD and lead to colectomy... as IBD patients are put on immunosuppression.
  • Organ transplant recipients.
  • HIV/AIDS.

Images:

• 

  CMV colitis - high. mag. (WC/Nephron)

• 

  CMV colitis - intermed. mag. (WC/Nephron)

Grossing

Types of specimens

Introduction to colorectal surgery:

1. Colonic resection - remove a piece of large bowel.
2. Total colectomy - leaves rectum and anus.
3. Subtotal colectomy - part of colon removed --or-- some of the rectum remains.
4. Right hemicolectomy - right colon + distal ileum.
5. Lower anterior resection (LAR) - proximal rectum +/- sigmoid (for proximal rectal malignancies).
   • Specimens have should have intact mesorectum - total mesorectal excision (TME) - reduces local recurrence.
6. Abdominoperineal resection (APR) - anus + rectum - results in a permanent stoma (for distal rectal malignancies).
7. Stoma - these are often done emergently and then get cut-out after the patient's condition has settled.

Images

• 

  APR specimen - anterior (WC)

• 

  APR specimen - lateral (WC)

• 

  APR specimen - inked (WC)

Identifying the specimen

• Transverse colon - has omentum.
• Ascending colon - usu. comes with ileocecal valve and a bit of ileum.
• Descending colon - has a bare area.
• Rectum - has adventitia.
  • Pathologists define it as starting where the adventitia starts/the serosal surface no longer completely surrounds the large intestine.
  • Anatomists define it in relation to the third sacral vertebra.

Images

• 

  Aigmoid and rectum. APR specimen. (WC)

Lymph nodes

• One should get at least 12 lymph nodes if it is cancer.

Quirke method

• Bowel is not opened - it is fixed... then sliced.

Standard method

• Bowel is prep'ed by opening it along the antimesenteric side.
• Dimensions - length, circumference at both margins.
• Radial margin/circumferential margin - should be painted.
  • Rectum starts/sigmoid ends @ place where serosa ends on the posterior aspect of the bowel.
    • The proximal, anterior aspect of the rectum has serosa, i.e. it is not painted.

Common non-neoplastic disease

Colorectal polyps

Main article: Intestinal polyps

Polyps are the bread & butter of GI pathology. They are very common.

Main types:

• Hyperplastic - most common, benign.
• Adenomatous - quite common, pre-malignant.
• Hamartomatous - rare, weird & wonderful.
• Inflammatory, AKA inflammatory pseudopolyps - associated with IBD.

Most common (images):

• 

  Hyperplastic polyp image - intermed. mag. (WC/Nephron)

• 

  Hyperplastic polyp image - low mag. (WC/Nephron)

Ischemic colitis

• AKA colonic ischemia.
• AKA ischemia of the colon.

General

• May occur together with ischemic enteritis, in which case it is known as ischemic enterocolitis.

Etiology - anything that leads to vascular occlusion:

• Atherosclerosis.
• Vasculitis.
• Embolization, e.g. thrombotic, foreign body.

Possible associated pathology:

• Necrotizing enteritis - necrosis of the small bowel only.
• Necrotizing enterocolitis - necrosis of the small and large bowel.

Closely related:

• Radiation colitis.
• Infectious colitis.

Note:

• Ischemia = compromised blood supply.

Gross

Features - location:

• Luminal part (mucosa & submucosa) affected - edema.
• Splenic flexture of colon commonly affected (vascular watershed).

Note:

• May have pseudomembranes (classically assoc. with C. difficile colitis), i.e. mimics an infectious process.
• DDx for pseudomembranes:
  • C. difficile induced pseudomembranous colitis.
  • Ischemic colitis.
  • Volvulus.
  • Necrotizing infections.
  • ... anything that causes severe mucosal injury.
• Radiologic correlate = bowel wall thickening.

Microscopic

Features:

• Withering crypts - important.
  • Colonic epithelium has decreased cytoplasm - NC ratio increased.
  • Usually with decreased goblet cells.
• Crypt loss/drop-out.
  • Less intestinal crypts present.
• Lamina propria hyalinization.
  • Dense pink material replaces loose connective tissue.
• Submucosa hyalinization.
• +/-Pseudomembranes (microscopic):
  • Loss of surface epithelium.
  • PMNs in lamina propria.
  • +/-Capillary fibrin thrombi.

Note:

• Pseudomembranes arise from the crypts - considered acute.

DDx:

• Inflammatory bowel disease.
• Radiation colitis.
• Toxins/drugs.
  • Rosuvastatin.
  • Cocaine.
  • NSAID overdose.
• Infectious colitis.

Images

• 

  Ischemic colitis - low mag. (WC/Nephron)

• 

  Ischemic colitis - high mag. (WC/Nephron)

• 

  Ischemic colitis - very high mag. (WC/Nephron)

• 

  Colonic pseudomembranes - low mag. (WC/Nephron)

• 

  Colonic pseudomembranes - intermed. mag. (WC/Nephron)

www:

• Ischemic colitis (flickr.com/euthman).
• Ischemic colitis (uchc.edu).

Sign out

Biopsy

TRANSVERSE COLON, BIOPSY:
- SEVERE ACTIVE COLITIS WITH ATTENUATED EPITHELIAL CYTOPLASM AND ULCERATION.
- CELLULAR DEBRIS.
- NEGATIVE FOR DYSPLASIA AND NEGATIVE FOR MALIGNANCY.

COMMENT:
The attenuated cytoplasm is compatible with ischemia; however, it is not 
accompanied with other suggestive findings (crypt drop out, lamina propria 
fibrosis, pseudomembranes).  The crypt architecture is test tube-like.

The differential diagnosis includes: ischemia, drug reaction, infectious 
etiologies and, less likely, inflammatory bowel disease. Clinical 
correlation is required.

COLON, SPLENIC FLEXURE, BIOPSY:
- PATCHY MODERATE ACTIVE COLITIS WITH ATTENUATED EPITHELIAL CYTOPLASM,
  FOCALLY DECREASED GOBLET CELLS AND ULCERATION.
- NEGATIVE FOR DYSPLASIA AND NEGATIVE FOR MALIGNANCY.

COMMENT:
The findings are consistent with ischemia; however, they are not diagnostic.

The differential diagnosis includes: ischemia, drug reaction, infectious
etiologies and, less likely, inflammatory bowel disease. Clinical
correlation is required.

Short version

LEFT COLON AND SIGMOID COLON, RESECTION:
- PSEUDOMEMBRANOUS COLITIS, SEE COMMENT.
- ONE LYMPH NODE NEGATIVE FOR MALIGNANCY ( 0 POSITIVE / 1 ).
- NEGATIVE FOR DYSPLASIA AND NEGATIVE FOR MALIGNANCY.

COMMENT:
Pseudomembrane formation is a non-specific finding.  It is consistent with ischemia;
however, it may be seen in other contexts, including infection. Clinical correlation is
required.

Long version

RECTOSIGMOID, RESECTION:
- BOWEL WALL ISCHEMIA WITH PERFORATION, SEROSITIS, MICROABSCESS FORMATION AND FOCAL 
POORLY FORMED PSEUDOMEMBRANES.
- NEGATIVE FOR MALIGNANCY.
- PLEASE SEE COMMENT.

COMMENT:
There is no evidence of inflammatory bowel disease:
The unaffected mucosa does not have obvious architectural distortion. No granulomas are
identified. The inflammation is largely associated with necrosis/ischemic changes
and favoured to be reactive.

The poorly formed pseudomembranes are associated with mural ischemic changes; they do not
specifically suggest an infection in this context.

The blood vessels do not show a vasculitis, or significant atherosclerosis.  Thrombi are
seen on several sections and found predominantly in the (smaller) veins. 

Considerations are thrombosis, thromboembolism, mechanical vascular compromise, and
infectious etiologies.  A vascular compromise is favoured as the underlying cause. 

Clinical and radiologic correlation is suggested.

Another long version

SIGMOID COLON, RESECTION:
- BOWEL WALL ISCHEMIA WITH PERFORATION, SEROSITIS, AND FOCAL POORLY FORMED
PSEUDOMEMBRANES.
- MILD ATHEROSCLEROSIS.
- DIVERTICULAR DISEASE.
- TWO LYMPH NODES NEGATIVE FOR MALIGNANCY ( 0 POSITIVE / 2 ).
- PLEASE SEE COMMENT.

COMMENT:
The sections show the changes of acute and chronic ischemic colitis (submucosal fibrosis,
lamina propria hyalinization, focal crypt drop-out, decreased goblet cells, pigmented
macrophages in the lamina propria, intraepithelial neutrophils).

No granulomas are identified. The inflammation is largely associated with
the necrosis/ischemic changes and favoured to be reactive.

The poorly formed pseudomembranes are associated with mural ischemic changes; they do not
specifically suggest an infectious etiology in this context.

The blood vessels do not show a vasculitis. However, focal neutrophilic perivascular 
inflammation is seen; this is probably a reactive process. No vascular thrombi are 
identified.

The findings are compatible with perforation secondary to a foreign body in the setting of
chronic ischemia.

Micro

Negative for ischemic colitis

The sections show colorectal mucosa with preservation of the crypt density and epithelium with a normal nuclear-to-cytoplasm ratio. There is no apparent lamina propria hyalinization. The muscularis mucosa is prominent. Focally, lymphoid aggregates are present.

No cryptitis is present. Neutrophils are not apparent in the lamina propria. No erosions are identified.

The epithelium matures appropriately from the crypt base to the surface.

Diverticular disease

Diverticulitis redirect here.

• AKA diverticulosis.

General

• Very common.

Complications:

• Diverticulitis.
• Diverticular-associated colitis - rare.
  • Rectal biopsy to differentiate from ulcerative colitis.

Gross

• Corrugated - like cardboard.
• Wall thickening (reactive).

Endoscopic image

• 

  Diverticular disease. (WC/Samir)

Grossing notes

• pp:Diverticular disease.

Microscopic

Features:

• Mucosa/submucosa invagination into the musuclaris propria (MP).
  • At the site the blood vessels supplying the mucosa and submucosa penetrate the MP.

Image:

• DD (wikispaces.com).

Sign out

RECTO-SIGMOID, LARGE BOWEL RESECTION:
- PERFORATED DIVERTICULITIS WITH SEROSITIS AND ABSCESS FORMATION.
- SUBMUCOSAL FIBROSIS.
- ONE LYMPH NODE NEGATIVE FOR MALIGNANCY ( 0 POSITIVE / 1 ).
- NEGATIVE FOR MALIGNANCY.

SIGMOID COLON, SIGMOIDECTOMY:
- DIVERTICULAR DISEASE WITHOUT DIVERTICULITIS.
- NEGATIVE FOR MALIGNANCY.

Pseudomembranous colitis

General

• Pseudomembranous colitis is a histomorphologic description which has a DDx. In other words, it can be caused by a number of things.

DDx of pseudomembranous colitis:

• C. difficile.
  • Known as C. difficile colitis.
• Ischemic colitis.
  • Volvulus.
• Other infections.

Etiology:

• Anything that causes a severe mucosal injury.

Gross

Features:

• Pseudomembranes:
  • Pale yellow (or white) irregular, raised mucosal lesions.
  • Early lesions: typical <10 mm.
• Interlesional mucosa often near normal grossly.

Images:

• Pseudomembranous colitis - endoscopic image (WP/Samir).

• 

  Pseudomembranous colitis (WC/Nephron)

Microscopic

Features:

• Heaped necrotic surface epithelium.
  • Described as "volanco lesions" - this is what is seen endoscopically.
• PMNs in lamina propria.
• +/-Capillary fibrin thrombi.

Notes:

• Pseudomembranes arise from the crypts.
• Rarely have (benign) signet ring cell-like cells.

Images

• 

  Pseudomembranes - low mag. (WC/Nephron)

• 

  Pseudomembranes - intermed. mag. (WC/Nephron)

www:

• Pseudomembranous colitis (upmc.edu).

Volvulus

General

• Uncommonly comes to pathology.
• It is essentially a radiologic diagnosis.
• In the context of autopsy, it is a gross diagnosis.

Gross

• Intestine folded over itself - typically leads to ischemia.

Images:

• Cecal volvulus (utah.edu).
• Volvulus (uic.edu).

Microscopic

Features:

• +/-Ischemic changes and/or necrosis.

DDx - essentially anything that causes ischemia:

• Embolus.
• Thrombosis.
• Vasculitis.

Sign out

RECTOSIGMOID, RESECTION:
- MURAL ISCHEMIA WITH PERFORATION, SEROSITIS, MICROABSCESS FORMATION AND POORLY FORMED PSEUDOMEMBRANES.
- SUBMUCOSAL FIBROSIS.
- NEGATIVE FOR MALIGNANCY.

COMMENT:
The findings are consistent with volvulus and the submucosal fibrosis suggests this may have been recurrent.

Inflammatory diseases

Inflammatory bowel disease

Main article: Inflammatory bowel disease

The bread 'n butter of gastroenterology. A detailed discussion of IBD is in the inflammatory bowel disease article. It comes in two main flavours (Crohn's disease, ulcerative colitis).

Microscopic

Features helpful for the diagnosis of IBD - as based on a study:

• Basal, i.e. crypt base, plasmacytosis with severe chronic inflammation,
• Crypt architectural abnormalities, and
• Distal Paneth cell metaplasia.
  • Paneth cells should not be in the left colon - if you see 'em think of IBD and other long-standing injurious processes.
  • Some claim that (friendly right colonic) paneth cells and paneth cell metaplasia look quite different and can be distinguished.

Microscopic colitis

Microscopic colitis may refer to a microscopic manifestation of an unspecified disease process that can be apparent macroscopically. This section deals with a pair of diseases (lymphocytic colitis and collagenous colitis) that are considered to only have microscopic manifestations and characteristic clinical presentation.

General

Presentation:

• Chronic diarrhea, non-bloody.

Notes:

• Clinical DDx includes irritable bowel syndrome - which has no or subtle histopathologic changes.

Classification

• Lymphocytic colitis (LC).
• Collagenous colitis (CC).

Note:

• Some believe that LC and CC are different time points in the same process-- but this is unproven.

Epidemiology

• Age: a disease of adults - usually 50s.
• Sex:
  • LC males ~= females,
  • CC females:males = 20:1.
• Drugs are associated with LC and CC.
  • NSAIDs - posulated association/weak association,
  • SSRIs (used primarily for depression) - moderate association, dependent on specific drug.
• Associated with autoimmune disorders - celiac disease, diabetes mellitus, thyroid disorders and arthritis.
• No increased risk of colorectal carcinoma.

Treatment

• Sometimes just follow-up.
• Steroids - budesonide -- short-term treatment.

Gross

• As the name suggests, they are microscopic, i.e. endoscopic examination is normal.

Microscopic

Lymphocytic colitis

Features:

• Lots of intraepithelial lymphocytes (>=20/100 lymphocytes/surface epithelial cells) and
• Lymphocytes in the lamina propria.

Significant negatives:

• No neutrophils.
• No crypt distortion.

DDx:

• Infectious colitis - neutrophils present... not lymphocytes.
• Collagenous colitis - has a band of collagen below the epithelium.

Image:

• LC (nih.gov).

Collagenous colitis

Features:

• Intraepithelial lymphocytes, and
• lymphocytes in the lamina propria.
• Collagenous material in the lamina propria (pink on H&E) -- key feature.
  • Can be demonstrated with a trichrome stain -- collagen = green on trichrome.
  • Subepithelial collagen needs to be >= 10 micrometres thick for diagnosis.
    • 8 micrometres is the diameter of a RBC.
    • The normal thickness of the subepithelial collagen is 3 micrometres.
    • Transverse colon usually thickest - in one series ~47 micrometres on average.
      • Thickening is usually patchy.
  • Thickening "follows the crypts from the surface" - useful for differentiating from tangential sections of the basement membrane.
  • Collagen may envelope capillaries - useful to discern from basement membrane.

Notes:

• CC is typically more prominent in the proximal colon - may reflect concentration gradient of offending causitive agents.
• Significant negative findings:
  • No PMNs.
  • No crypt distortion.
• Thickened collagen band uncommon in rectum.

Images

• 

  CC - intermed mag. (WC/Nephron)

• 

  CC - high mag. (WC/Nephron)

Sign out

TRANSVERSE COLON, BIOPSY:
- COLLAGENOUS COLITIS.

ASCENDING COLON, BIOPSY:
- LYMPHOCYTIC COLITIS.

Micro

Lymphocytic colitis

The sections show colonic mucosa with abundant intraepithelial lymphocytes (>20 lymphocytes/100 surface epithelial cells). The glandular architecture is within normal limits. No thickened collagen band is apparent below the epithelium.

There are no granulomas. No neutrophilic cryptitis is apparent. The epithelium matures appropriately to the surface.

Collagenous colitis

The sections show colonic mucosa with abundant intraepithelial lymphocytes (>20 lymphocytes/100 surface epithelial cells). A prominent collagen band is apparent below the epithelium (>10 micrometres thick). The glandular architecture is within normal limits.

There are no granulomas. No neutrophilic cryptitis is apparent. The epithelium matures appropriately to the surface.

Diversion colitis

Diversion proctitis redirect here.

General

• Segment of de-functioned bowel due to surgical diversion, i.e. stoma (ileostomy or colostomy).
• Diagnosis dependent on history - key point.

Gross

Features:

• Ulceration - classic.
• Surgical changes, e.g. fibrotic-appearing thickened wall.
  • May not be apparent.

Microscopic

Features:

• Follicular lymphoid hyperplasia - key feature.
  • Abundant lymphoid nodules.
• Plasma cells and lymphocytes.
• +/-Changes of an active colitis - uncommon:
  • Cryptitis.
  • Crypt abscesses.

Notes:

• May show IBD-like changes.
  • IBD should not be diagnosed on a diverted segment of bowel.

DDx:

• Inflammatory bowel disease - no stoma.
• Ischemic colitis.
• Infectious colitis.
  • Pseudomembranous colitis.

Images

• 

  Diversion proctitis - low mag. (WC/Nephron)

• 

  Diversion proctitis - high mag. (WC/Nephron)

Sign out

SIGMOID COLON, BIOPSIES:
- MILD ACTIVE COLITIS WITH LAMINA PROPRIA FIBROSIS, SEE COMMENT.
- NEGATIVE FOR DYSPLASIA.

COMMENT:
No granulomas are identified.  Follicular lymphoid hyperplasia is not identified;
however, there is no definite submucosa present.

Diverted segments of bowel can have inflammatory bowel disease-like changes.

In the context of a diverted segment of bowel, the findings are compatible with
a diversion colitis.

Eosinophilic colitis

General

• Rare.
• May be a component of eosinophilic gastroenteritis.

Clinical features:

• Abdominal pain
• Diarrhea +/-blood.
• +/-Weight loss.

Gross

Features - endoscopic:

• Edema.
• Granular appearance.

Microscopic

Features:

• Abundant eosinophils - no agreed upon number.
  • "Most use 20/HPF" - a definition that suffers from HPFitis.
    • There is variation along the large bowel - normal in rectum <10/HPF, normal in cecum <30/HPF (???).

DDx:

• Inflammatory bowel disease:
  • Crohn's disease.
  • Ulcerative colitis.
• Infection:
  • Pinworm.
  • Strongyloidiasis.
• Autoimmune disease:
  • Scleroderma.
  • Churg-Strauss syndrome.
  • Celiac disease.
• Drug reactions.

Image:

• EC (nih.gov).

Sign out

DESCENDING COLON, BIOPSY:
- COLONIC MUCOSA WITH MILD EOSINOPHILIA, SEE COMMENT.
- NEGATIVE FOR DYSPLASIA.

COMMENT:
Focally, there are up to 40 eosinophils / 0.2376 mm*mm (approx. field area at 400X). This
is a non-specific finding. No eosinophilic crypt abscesses are seen. No (neutrophilic)
cryptitis is present. Clinical correlation is suggested.

DESCENDING COLON, BIOPSY:
- COLONIC MUCOSA WITH MILD EOSINOPHILIA, SEE COMMENT.
- NEGATIVE FOR ACTIVE COLITIS.
- NEGATIVE FOR DYSPLASIA.

COMMENT:
There are up to 40 eosinophils / 0.2376 mm*mm (field area at 400X). This is a 
non-specific finding.  The differential diagnosis includes inflammatory bowel 
disease, infection (especially helminths), a drug reaction, and autoimmune 
disorders (e.g. Churg-Strauss syndrome, celiac disease, scleroderma). Clinical 
correlation is required.

Infectious

Infectious colitis

This section covers non-specific colitides that appear to have an infective etiology.

General

• Common.
• Diarrhea - typical symptom.

Gross

• +/-Erythema on endoscopy.

Microscopic

Features:

• Neutrophils predominant - key feature.
  • The neutrophils are often superficial - they go to were the bad guys are.
• No architectural distortion - if acute.

DDx:

• Inflammatory bowel disease - lymphoplasmacytic infiltrate predominant, usually has chronic changes.
• Ischemic colitis.
• Medications - focal neutrophils.
• Lymphocytic colitis - lymphocytes with a squiggly nucleus, may be confused with neutrophils.
• Specific causes of infective colitis - with a distinctive morphology.
  • CMV colitis - esp. in the immunodeficient.
  • Pseudomembranous colitis - usu. due to C. difficle, has characteristic gross & microscopic appearance.
  • Intestinal spirochetes.
  • Amebiasis.
  • Strongyloidiasis.
  • Cryptosporidiosis.

IHC

Done if the patient is immunosuppressed, or there is clinical or morphological suspicion:

• CMV.
• HSV-1.
• HSV-2.
• EBV - may mimic IBD.

Sign out

ASCENDING COLON, BIOPSY:
- MILD ACTIVE COLITIS, SEE COMMENT.

COMMENT:
There is are no granulomas.  The crypt architecture is normal.  A benign lymphoid nodule is
present.

The differential diagnosis includes infective etiologies, early inflammatory
bowel disease and ischemia.  The histomorphology is more in keeping with an infective
etiology as neutrophils are a predominant feature; however, clinical correlation is
required.

Cytomegalovirus colitis

Main article: CMV

• Abbreviated CMV colitis.

General

• Uncommon.
• Immunosuppressed population at risk, e.g. transplant recipients, individuals with HIV.

Microscopic

Features:

• Enlarged nucleus - classically in endothelial cells.

DDx:

• Infectious colitis without a distinctive morphology.
• CMV colitis superimposed on inflammatory bowel disease.

Images

• 

  CMV colitis - intermed. mag. (WC/Nephron)

• 

  CMV colitis - high mag. (WC/Nephron)

IHC

• CMV +ve.

Others:

• HSV-1.
• HSV-2.
• VZV.
• EBV.

Intestinal spirochetosis

• AKA intestinal spirochetes; more specifically colonic spirochetes, colonic spirochetosis.

General

• Caused by spirochetes - specifically Brachyspira piloicoli (previously Serpulina pilosicoli) and Brachyspira aalborgi.
• Very rare cause of diarrhea, associated with male homosexual behaviour.

Symptoms:

• Watery diarrhea, abdominal pain, +/-blood per rectum.

Treatment:

• Metronidazole.

Microscopic

Features:

• Hyperchromatic fuzz on luminal aspect of epithelial cells; at brush border.

DDx:

• Normal colon.
• Infectious colitis without a distinctive morphology.

Images

• 

  Intestinal spirochetes - cropped - very high mag. (WC/Nephron)

• 

  Intestinal spirochetes - very high mag. (WC/Nephron)

• 

  Intestinal spirochetes - intermed. mag. (WC/Nephron)

www:

• Intestinal spirochetosis & CMV colitis - several images (upmc.edu).

Special stains

• Silver stains highlight 'em (e.g. Warthin-Starry stain).

Amebiasis

• May also be spelling amoebiasis.

General

• Infection with Entamoeba histolytica.
• May mimic colon cancer.

May cause:

• Dysentery (diarrhea containing mucus and/or blood in the feces).
• Colitis.
• Liver abscess.

Microscopic

Features:

• Entamoeba histolytica are round/ovoid eosinophilic bodies ~ 40-60 micrometers in maximal dimension.
  • Found in bowel lumen.
  • Ingest RBCs.

Image

• 

  Amebiasis - very high mag. (WC/Nephron)

• 

  Amebiasis (WC)

Cryptosporidiosis

Main article: Cryptosporidiosis

General

• Usually in immune incompetent individuals, e.g. HIV/AIDS.

Microscopic

Features:

• Uniform spherical nodules 2-4 micrometres in diameter, typical location - GI tract brush border.
  • Bluish staining of brush border key feature - low power.

Rectal pathology

Solitary rectal ulcer

• AKA solitary ulcer syndrome of the rectum, abbreviated SUS.
• AKA solitary rectal ulcer syndrome.
• Mucosal prolapse syndrome may be used as a synonym; however, it encompasses other entities.

General

• Clinically may be suspected to a malignancy - biopsied routinely.
• Mucosal ulceration.
• "Three-lies disease":

1. May not be solitary.
2. May not be rectal -- can be in left colon.
3. May not be ulcerating -- non-ulcerated lesions: polypoid and/or erythematous.

Note: Each of the words in solitary rectal ulcer is a lie.

Epidemiology

• Typically younger patients - average age of presentation ~30 years in one study.
• Rare.

Clinical presentation

• Usually presents as BRBPR ~ 85% of cases.
• Abdominal pain present in approx. 1/3.
  • May be very painful.

Treatment:

• Usually conservative, i.e. non-surgical.
• Resection - may be done for fear of malignancy.

Gross

• Classically, anterior or anterolateral wall of the rectum.

Microscopic

Features:

• Fibrosis of the lamina propria.
• Thickened muscularis mucosa with abnormal extension to the lumen.
• +/-Mucosa ulceration.
• +/-Submucosal fibrosis.

DDx:

• Inflammatory pseudopolyp (inflammatory polyp).
  • Associated with inflammatory bowel disease.
• Rectal prolapse.
• Well-differentiated adenocarcinoma.

IHC

• p53 -ve.
  • May be used to help exclude adenocarcinoma.

Rectal prolapse

General

• Usually close to the anal verge.
• Rare forms can occasionally be confused with cancer.

Microscopic

Features:

• "Fibromuscular hyperplasia" - key feature:
  • Fibrosis (submucosa, lamina propria).
  • Muscularis mucosae is "too superficial" (muscle in the lamina propria).
• Surface ulceration + inflammation (neutrophils).
• +/-Serration of epithelium at the surface.

Notes:

• Important negative: no nuclear atypia.

Images

• 

  Rectal prolapse - low mag. (WC/Nephon)

• 

  Rectal prolapse - intermed. mag. (WC/Nephron)

Neoplastic disease

Colorectal Tumours

Main article: Colorectal tumours

These are very common. The are covered in a separate article entitled colorectal tumours.

Neuroendocrine tumour

Main article: Neuroendocrine neoplasms#GI tract

• AKA carcinoid.

Goblet cell carcinoid

Described in detail in the appendix article.

• AKA crypt cell carcinoma.
• Biphasic tumour; features of carcinoid tumour and adenocarcinoma.

Other

Pseudomelanosis coli

• AKA melanosis coli.

General

• Not melanin as the name melanosis coli suggests; it is actually lipofuscin (in macrophages).
• Endoscopist may see brown pigmentation of mucosa and suspect the diagnosis.
• Presence may lead to endoscopic misinterpretation of colitis severity.

Epidemiology

• Classically associated with anthracene containing laxative (e.g. Senokot) use and herbal remedies.
  • May be seen in individuals not using laxatives.
• Seen in (long-standing) inflammatory bowel disease, especially ulcerative colitis.

Gross

• Brown pigmentation of the mucosa, esp. cecum and proximal colon.

Image

• 

  Melanosis coli - endoscopic image (WC)

Microscopic

Features:

• Brown granular pigment - in the lamina propria.
  • Typically more prominent in the cecum and proximal colon.

Notes:

• DDx of brown pigment:
  • Lipofuscin - comes with age (can be demonstrated with a PAS stain or Kluver-Barrera stain).
    • Melanosis coli.
  • Old haemorrhage, i.e. hemosiderin-laden macrophages (may be demonstrated with Prussian blue stain).
  • Melanin (from melanocytes) - rare in colon (may be demonstrated with a Fontana-Masson stain -- though not so useful in the GI tract).
  • Foreign material (e.g. tattoo pigment) - not seen in GI tract.

Images

• 

  Melanosis coli - high mag. (WC/Nephron)

• 

  Melanosis coli - low mag. (WC/Nephron)

Stains

• Can be demonstrated with a PAS stain.

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ASCENDING COLON, BIOPSY:
- PSEUDOMELANOSIS COLI.
- NEGATIVE FOR ACTIVE COLITIS.
- NEGATIVE FOR DYSPLASIA.

Angiodysplasia

General

• Causes (lower) GI haemorrhage.
• Generally, not a problem pathologists see.
• May be associated with aortic stenosis; known as Heyde syndrome.

Epidemiology:

• Older people.

Etiology:

• Thought to be caused by the higher wall tension of cecum (due to larger diameter) and result from (intermittent) venous occlusion/focal dilation of vessels.

Gross

• Cecum - classic location.

Note:

• Crohn's disease - may mimic angiodysplasia radiographically.

Microscopic

Features:

• Dilated vessels in mucosa and submucosa.

Drugs

Main article: Drug toxicity

Sodium polystyrene sulfonate

• AKA Kayexalate.

General

• Used to treat hyperkalemia - as may be seen in renal failure.

Microscopic

Features:

• Purple blobs on H&E stain - look somewhat like calcium phosphate.
• Can cause focal necrosis.

Image

• 

  Adenocarcinoma and sodium polystyrene crystals (WC/Nephron)

Graft-versus host disease

Main article: Graft-versus-host disease

• Abbreviated as GVHD.
• Seen in the context of bone marrow transplants.

Bowel transplant

The histology of bowel transplant rejection is identical to GVHD - see GVHD.

Chronic constipation

This section deals with chronic constipation that has no apparent cause.

General

• This is occasionally an indication for colectomy.

General differential diagnosis for constipation:

• Tumour.
• Adhesions - due to previous surgery.
• Neuropathy.
  • Parkinson disease.
• Congenital defect (Hirschsprung's disease).
• Myopathy.
• Medications/substance use.
• Idiopathic.

Gross

• No changes.

Microscopic

Features:

• Colon within normal limits.
  • Look for the Ganglion cells (submucosal plexus, myenteric plexus).
  • Look for interstitial cells of Cajal (with CD117) - typically most common around the myenteric plexus.

Negatives:

• No significant vascular disease.
• No fibrosis.
• No loss of muscle.

Stains & IHC

Work-up if no tumour is identified:

• Routine H&E.
• Smooth muscle actin - confirm myocyte loss.
• Gomori trichrome - examine connective tissue.
• CD117 - to look for the interstitial cells of Cajal.
  • <50% the expected = abnormal.
    • Normal numbers not defined.
• HU - neuronal marker.

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• A long list of things to report is contained the recommendation of a working group.
  • Most pathology practises do not report much.

TERMINAL ILEUM, CECUM, COLON (ASCENDING, TRANSVERSE AND SIGMOID), COLECTOMY:
- SMALL BOWEL, CECUM, AND COLON WITHIN NORMAL LIMITS.
- FOUR LYMPH NODES NEGATIVE FOR MALIGNANCY ( 0 POSITIVE / 4 ).
- NEGATIVE FOR DYSPLASIA AND NEGATIVE FOR MALIGNANCY.

COMMENT:
Several stains were done:
 CD117: interstitial cells of Cajal present, no apparent decrease.
 SMA: no significant myocyte loss.
 Gomori trichrome: no abnormal fibrosis apparent.
 Tau: no abnormalities apparent.

See also

• GIST.
• Gastrointestinal pathology.
• Intestinal polyps.
• Small bowel.