HPFitis is use of HPF (high-power field) without proper qualification, i.e. without giving the field area.
HPF redirects here.
Its etiology is thought to be either:
- Ignorance of statistics and sampling theory.
- A combination of the above two.
It appears to have a higher incidence in math phobic individuals.
Consequences of HPFitis
- Papers on eosinophilic esophagitis are not comparable.
- GISTs were improperly staged - until they standardized to 5 mm2.
- Mitotic score in the Nottingham score for invasive breast cancer is based on the pseudo-standard area of "10 HPFs"; this results in significant variance due to the field area of the microscope in large cohorts.
Most modern microscopes, have an eye piece diameter of 22 mm. Therefore, the field diameter at 40 X is approximately 22 mm / 40 X ~= 0.55 mm and the field of view is pi/4*(0.55 mm)2 = 0.2376 mm2.
- Dellon ES, Aderoju A, Woosley JT, Sandler RS, Shaheen NJ (October 2007). "Variability in diagnostic criteria for eosinophilic esophagitis: a systematic review". Am. J. Gastroenterol. 102 (10): 2300–13. doi:10.1111/j.1572-0241.2007.01396.x. PMID 17617209.
- Bonert, M.; Tate, AJ. (Feb 2017). "Mitotic counts in breast cancer should be standardized with a uniform sample area.". Biomed Eng Online 16 (1): 28. doi:10.1186/s12938-016-0301-z. PMC 5312435. PMID 28202066. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5312435/.