Difference between revisions of "Inflammatory bowel disease"

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*Surveillance biopsies should specify the (anatomical) site - so, it possible to find any site of interest on a follow-up colonoscopy.<ref name=pmid16609751>{{Cite journal  | last1 = Panaccione | first1 = R. | title = The approach to dysplasia surveillance in inflammatory bowel disease. | journal = Can J Gastroenterol | volume = 20 | issue = 4 | pages = 251-3 | month = Apr | year = 2006 | doi =  | PMID = 16609751 | PMC = 2659899}}</ref>
*Surveillance biopsies should specify the (anatomical) site - so, it possible to find any site of interest on a follow-up colonoscopy.<ref name=pmid16609751>{{Cite journal  | last1 = Panaccione | first1 = R. | title = The approach to dysplasia surveillance in inflammatory bowel disease. | journal = Can J Gastroenterol | volume = 20 | issue = 4 | pages = 251-3 | month = Apr | year = 2006 | doi =  | PMID = 16609751 | PMC = 2659899}}</ref>


===Biopsies all submitted it all in one bottle===
===Biopsies all submitted in one bottle===
<pre>
<pre>
COLON (SITE NOT FURTHER SPECIFIED), BIOPSIES:
COLON (SITE NOT FURTHER SPECIFIED), BIOPSIES:
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====Images====
====Images====
<gallery>
<gallery>
Image:Crohn%27s_disease_-_colon_-_high_mag.jpg | Crohn's disease - beautiful granulomas in the colon - high mag. (WC)
Image:Crohn%27s_disease_-_colon_-_high_mag.jpg | Crohn's disease - very well-formed granulomas in the [[colon]] - high mag. (WC)
Image:Crohn%27s_disease_-_duodenum_-_intermed_mag.jpg | Crohn's disease - duodenum - intermed. mag. (WC)
Image:Crohn%27s_disease_-_duodenum_-_intermed_mag.jpg | Crohn's disease - duodenum - intermed. mag. (WC)
Image: Cryptitis_-_alt_--_very_high_mag.jpg | Cryptitis. (WC)
Image: Cryptitis_-_alt_--_very_high_mag.jpg | Cryptitis. (WC)
Image:Crypt_branching_high_mag.jpg | Crypt branching. (WC)
Image:Crypt_branching_high_mag.jpg | Crypt branching. (WC)
</gallery>
</gallery>
===Grading===
===Grading===
*Several systems exists.<ref name=Kirsch>Kirsch, R. 13 December 2010.</ref>
*Several systems exists.<ref name=Kirsch>Kirsch, R. 13 December 2010.</ref>
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=Sign out=
=Sign out=
===Quiescent inflammatory bowel disease===
===Quiescent inflammatory bowel disease===
Used when:
*No accepted formal definition.
 
May be used when:
#Non-specific "minimal abnormalities" are present.
#Non-specific "minimal abnormalities" are present.
#There is a history of inflammatory bowel disease.
#There is a history of inflammatory bowel disease.
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*Macrophages in the lamina propria.
*Macrophages in the lamina propria.
*Lymphoid nodules.
*Lymphoid nodules.
*Abundant plasma cells in the lamina propria.
*"Abundant" plasma cells in the lamina propria.
**''Abundant'' is subjective.


<pre>
<pre>
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No definite granulomata are identified. Architectural changes, including crypt drop out,
No definite granulomata are identified. Architectural changes, including crypt drop out,
are present. Lamina propria plasma cells are abundant throughout the biopsy and eosinophil  
are present. Lamina propria plasma cells are abundant throughout the biopsy and eosinophil  
numbers are mildly increased. Lymphoid aggregates with germinal centre formation are present. All fragments of tissue are affected.
numbers are mildly increased. Lymphoid aggregates with germinal centre formation are  
present. All fragments of tissue are affected.


The findings are compatible with inflammatory bowel disease or an infectious
The findings are compatible with inflammatory bowel disease or an infectious
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==Ulcerative colitis==
==Ulcerative colitis==
*Often abbreviated as ''UC''.
*Often abbreviated as ''UC''.
===General===
{{Main|Ulcerative colitis}}
*May be associated with ''[[toxic megacolon]]''.
 
Epidemiology:
*Associated with ''[[primary sclerosing cholangitis]]''.
*[[Appendicitis]] is considered protective against UC.<ref name=pmid19685454>{{Cite journal  | last1 = Beaugerie | first1 = L. | last2 = Sokol | first2 = H. | title = Appendicitis, not appendectomy, is protective against ulcerative colitis, both in the general population and first-degree relatives of patients with IBD. | journal = Inflamm Bowel Dis | volume =  | issue =  | pages =  | month = Aug | year = 2009 | doi = 10.1002/ibd.21064 | PMID = 19685454 }}</ref><ref name=pmid19273505>{{Cite journal  | last1 = Timmer | first1 = A. | last2 = Obermeier | first2 = F. | title = Reduced risk of ulcerative colitis after appendicectomy. | journal = BMJ | volume = 338 | issue =  | pages = b225 | month =  | year = 2009 | doi =  | PMID = 19273505 }}</ref>
*[[Smoking]] is protective; the opposite is true for [[Crohn's disease]].<ref name=pmid19273505/>
 
===Gross===
*Conventionally considered to be contiguous, i.e. no "skip lesions", with rectal involvement being most severe.
*Dependent on the study one reads... rectal sparing may be seen in 15% of UC patients.<ref>{{cite journal |author=Bernstein CN, Shanahan F, Anton PA, Weinstein WM |title=Patchiness of mucosal inflammation in treated ulcerative colitis: a prospective study |journal=Gastrointest. Endosc. |volume=42 |issue=3 |pages=232-7 |year=1995 |month=September |pmid=7498688 |doi= |url=}}</ref>
 
===Microscopic===
Features:
*Inflammation:
**Active:
***Neutrophils:
****Intraepithelial ([[cryptitis]]).†
****Clusters in crypts ([[crypt abscesses]]).
****Erosions.
**Chronic:
***Architectural distortion.
***Basal plasmacytosis.
***Foveolar metaplasia.
***Paneth cell metaplasia (distal).
**Lack of [[granulomas]].
*Mucin depletion - common in UC.<ref name=pmid2318990>{{Cite journal  | last1 = McCormick | first1 = DA. | last2 = Horton | first2 = LW. | last3 = Mee | first3 = AS. | title = Mucin depletion in inflammatory bowel disease. | journal = J Clin Pathol | volume = 43 | issue = 2 | pages = 143-6 | month = Feb | year = 1990 | doi =  | PMID = 2318990 }}</ref>
 
Notes:
*†Neutrophils are usually numerous in the lamina propria in minimal/mild active inflammation.
*No full wall-thickness inflammation.
*Epithelial apoptosis correlated with inflammation.<ref name=pmid19958058>{{Cite journal  | last1 = Seidelin | first1 = JB. | last2 = Nielsen | first2 = OH. | title = Epithelial apoptosis: cause or consequence of ulcerative colitis? | journal = Scand J Gastroenterol | volume = 44 | issue = 12 | pages = 1429-34 | month =  | year = 2009 | doi = 10.3109/00365520903301212 | PMID = 19958058 }}</ref>
 
DDx:
*[[Crohn's disease]].
*[[Infectious colitis]].
*[[Ischemic colitis]].
*[[Diversion colitis]].
 
===Sign out===
<pre>
SIGMOID COLON, BIOPSY:
- MODERATE ACTIVE COLITIS WITH CHRONIC CHANGES, SEE COMMENT.
- NEGATIVE FOR DYSPLASIA.
 
COMMENT:
No granulomata are identified. The sampled mucosa is diffusely inflamed. Crypt drop-out and
architectural distortion are present.
 
The findings are consistent with inflammatory bowel disease; however, an infectious etiology
should be considered as a possibility.
</pre>
 
<pre>
SIGMOID COLON, BIOPSY:
- MILD ACTIVE COLITIS, SEE COMMENT.
- NEGATIVE FOR DYSPLASIA.
 
COMMENT:
No granulomata are identified.
</pre>
 
<pre>
A. RIGHT COLON, BIOPSY:
- MODERATE ACTIVE COLITIS, SEE COMMENT.
- NEGATIVE FOR DYSPLASIA.
 
B. LEFT COLON, BIOPSY:
- MODERATE-TO-SEVERE CHRONIC ACTIVE COLITIS, SEE COMMENT.
- NEGATIVE FOR DYSPLASIA.
 
COMMENT:
No granulomata are identified. The mucosa is diffusely inflamed. Architectural distortion
is present in the left colon.  The findings are consistent with ulcerative colitis;
however, an infectious etiology should be considered as a possibility.
</pre>
 
<pre>
RECTUM, BIOPSY:
- MODERATE DIFFUSE CHRONIC ACTIVE PROCTITIS.
- NEGATIVE FOR DYSPLASIA.
 
COMMENT:
No definite granulomata are identified. Crypt drop-out is present.
Within the proper clinical context, these are findings of
inflammatory bowel disease.
</pre>
 
====Inactive disease====
<pre>
SIGMOID COLON, BIOPSY:
- CHRONIC COLITIS, SEE COMMENT.
- NEGATIVE FOR ACTIVE COLITIS.
- NEGATIVE FOR DYSPLASIA.
 
COMMENT:
The sections show chronic changes (basal plasmacytosis, marked crypt architectural
distortion, crypt branching); however, no active colitis is present. Also, lamina propria
neutrophils, which are often easy to identify in an active colitis, are not apparent.
Appreciable numbers of lamina propria eosinophils are present and focally intraepithelial.
No granulomas are identified. Clinical correlation is required.
</pre>
 
====Surveillance====
<pre>
A. ASCENDING COLON, BIOPSY:
- COLONIC MUCOSA WITHOUT APPARENT PATHOLOGY.
- NEGATIVE FOR ACTIVE COLITIS.
- NEGATIVE FOR DYSPLASIA.
 
B. TRANSVERSE COLON, BIOPSY:
- COLONIC MUCOSA WITHOUT APPARENT PATHOLOGY.
- NEGATIVE FOR ACTIVE COLITIS.
- NEGATIVE FOR DYSPLASIA.
 
C. DESCENDING COLON, BIOPSY:
- COLONIC MUCOSA WITHOUT APPARENT PATHOLOGY.
- NEGATIVE FOR ACTIVE COLITIS.
- NEGATIVE FOR DYSPLASIA.
 
D. SIGMOID COLON, BIOPSY:
- COLONIC MUCOSA WITHOUT APPARENT PATHOLOGY.
- NEGATIVE FOR ACTIVE COLITIS.
- NEGATIVE FOR DYSPLASIA.
 
E. RECTUM, BIOPSY:
- RECTAL MUCOSA WITHOUT APPARENT PATHOLOGY.
- NEGATIVE FOR ACTIVE PROCTITIS.
- NEGATIVE FOR DYSPLASIA.
 
COMMENT:
Morphologically benign lymphoid aggregates are found focally. No granulomas are
identified. Minimal architectural changes are seen focally.
</pre>
 
<pre>
A. CECUM, BIOPSY:
- QUIESCENT INFLAMMATORY BOWEL DISEASE.
- NEGATIVE FOR DYSPLASIA.
 
B. ASCENDING COLON, BIOPSY:
- QUIESCENT INFLAMMATORY BOWEL DISEASE.
- NEGATIVE FOR DYSPLASIA.
 
C. COLON, HEPATIC FLEXURE, BIOPSY,
- QUIESCENT INFLAMMATORY BOWEL DISEASE.
- NEGATIVE FOR DYSPLASIA.
 
D. TRANSVERSE COLON, BIOPSY:
- QUIESCENT INFLAMMATORY BOWEL DISEASE.
- NEGATIVE FOR DYSPLASIA.
 
E. COLON, SPLENIC FLEXURE, BIOPSY:
- QUIESCENT INFLAMMATORY BOWEL DISEASE.
- NEGATIVE FOR DYSPLASIA.
 
F. DESCENDING COLON, BIOPSY:
- QUIESCENT INFLAMMATORY BOWEL DISEASE.
- NEGATIVE FOR DYSPLASIA.
 
G. SIGMOID COLON, BIOPSY:
- QUIESCENT INFLAMMATORY BOWEL DISEASE.
- NEGATIVE FOR DYSPLASIA.
 
H. RECTUM, BIOPSY:
- QUIESCENT INFLAMMATORY BOWEL DISEASE.
- NEGATIVE FOR DYSPLASIA.
 
COMMENT:
No granulomas are identified. Mild architectural distortion is present. No active
inflammation is identified. Scattered mucosal lymphoid nodules with germinal center
formation are present.
</pre>
 
====Granulomas and inflamed crypts - clinically UC====
<pre>
A. CECUM, BIOPSY:
- ACTIVE CECITIS, MILD.
- SMALL MUCOSAL GRANULOMAS, SUPERFICIAL, SEE COMMENT.
- NEGATIVE FOR DYSPLASIA.
 
...
 
COMMENT - PART A:
The small granulomas are mucosal and near, but not all adjacent to, inflamed crypts; this
finding raises the possibility of Crohn's disease. It should be noted that mucosal
granulomas may be seen in ulcerative colitis beside inflamed crypts.
 
COMMENT - GENERAL:
The inflammation is diffuse and chronic changes are seen throughout. Distal paneth cell
metaplasia is present. The diffuse nature of the inflammation would be more in keeping with
ulcerative colitis. Clinical correlation is required.
</pre>
 
====Micro====
The sections show focal intraepithelial neutrophils (cryptitis).  No crypt abscesses are identified. Granulation tissue is present. There is focal Paneth cell metaplasia and foveolar metaplasia. No granulomata are identified.


==Crohn's disease==
==Crohn's disease==
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#CUTE = Colitis of uncertain type or etiology.
#CUTE = Colitis of uncertain type or etiology.
#*Should be reserved for resection specimens only.
#*Should be reserved for resection specimens only.


==Dysplasia in inflammatory bowel disease==
==Dysplasia in inflammatory bowel disease==
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Notes:
Notes:
*GI experts and generalists have similar rates agreement.<ref name=pmid11400142>{{Cite journal  | last1 = Eaden | first1 = J. | last2 = Abrams | first2 = K. | last3 = McKay | first3 = H. | last4 = Denley | first4 = H. | last5 = Mayberry | first5 = J. | title = Inter-observer variation between general and specialist gastrointestinal pathologists when grading dysplasia in ulcerative colitis. | journal = J Pathol | volume = 194 | issue = 2 | pages = 152-7 | month = Jun | year = 2001 | doi = 10.1002/path.876 | PMID = 11400142 }}</ref>
*GI experts and generalists have similar rates of agreement.<ref name=pmid11400142>{{Cite journal  | last1 = Eaden | first1 = J. | last2 = Abrams | first2 = K. | last3 = McKay | first3 = H. | last4 = Denley | first4 = H. | last5 = Mayberry | first5 = J. | title = Inter-observer variation between general and specialist gastrointestinal pathologists when grading dysplasia in ulcerative colitis. | journal = J Pathol | volume = 194 | issue = 2 | pages = 152-7 | month = Jun | year = 2001 | doi = 10.1002/path.876 | PMID = 11400142 }}</ref>


===Microscopic===
===Microscopic===
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==Dysplasia-associated lesion or mass==
==Dysplasia-associated lesion or mass==
*Abbreviated ''DALM''.
*Abbreviated ''DALM''.
===General===
{{Main|Dysplasia-associated lesion or mass}}
*Proving invasive malignancy (on histopathologic grounds alone) in the setting of chronic inflammation is difficult.<ref name=pmid7450425>{{Cite journal  | last1 = Blackstone | first1 = MO. | last2 = Riddell | first2 = RH. | last3 = Rogers | first3 = BH. | last4 = Levin | first4 = B. | title = Dysplasia-associated lesion or mass (DALM) detected by colonoscopy in long-standing ulcerative colitis: an indication for colectomy. | journal = Gastroenterology | volume = 80 | issue = 2 | pages = 366-74 | month = Feb | year = 1981 | doi =  | PMID = 7450425 }}</ref>
*This diagnosis depends on correlation of endoscopy and histopathology - '''important'''.<ref name=pmid21912466>{{Cite journal  | last1 = Neumann | first1 = H. | last2 = Vieth | first2 = M. | last3 = Langner | first3 = C. | last4 = Neurath | first4 = MF. | last5 = Mudter | first5 = J. | title = Cancer risk in IBD: how to diagnose and how to manage DALM and ALM. | journal = World J Gastroenterol | volume = 17 | issue = 27 | pages = 3184-91 | month = Jul | year = 2011 | doi = 10.3748/wjg.v17.i27.3184 | PMID = 21912466 }}</ref>
**Biopsies are usually taken of the lesion and around the base.
*This diagnosis usually leads to a [[colectomy]].
 
===Gross===
*Endoscopically "suspicious", i.e. endoscopist thinks this is a DALM - '''essential feature'''.
**Usually have a positive lifting sign.
===Microscopic===
Features:
*Cytologic dysplasia - as in [[adenomatous polyps]] - '''key feature'''.
*Flat or polypoid.<ref name=pmid7450425/>
 
DDx:
*Sporadic [[adenomatous polyp]] -- favouring sporadic:
**Sharp transition between lesion and the surrounding tissue.<ref name=pmid21912466/>
**Polyps not at site of active disease.<ref name=pmid10746669>{{Cite journal  | last1 = Fogt | first1 = F. | last2 = Urbanski | first2 = SJ. | last3 = Sanders | first3 = ME. | last4 = Furth | first4 = EE. | last5 = Zimmerman | first5 = RL. | last6 = Deren | first6 = JJ. | last7 = Noffsinger | first7 = AE. | last8 = Vortmeyer | first8 = AO. | last9 = Hartmann | first9 = CJ. | title = Distinction between dysplasia-associated lesion or mass (DALM) and adenoma in patients with ulcerative colitis. | journal = Hum Pathol | volume = 31 | issue = 3 | pages = 288-91 | month = Mar | year = 2000 | doi =  | PMID = 10746669 }}</ref>
 
Image:
*[http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3158393/figure/F7/ DALM (nlm.nih.gov)].<ref name=pmid21912466/>


==Pouchitis==
==Pouchitis==
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**Generally, pouches are ''not'' used in Crohn's disease.
**Generally, pouches are ''not'' used in Crohn's disease.
*Chronic pouchitis seen in approximately 15% of patients.<ref name=pmid12617884 >{{Cite journal  | last1 = Gionchetti | first1 = P. | last2 = Amadini | first2 = C. | last3 = Rizzello | first3 = F. | last4 = Venturi | first4 = A. | last5 = Poggioli | first5 = G. | last6 = Campieri | first6 = M. | title = Diagnosis and treatment of pouchitis. | journal = Best Pract Res Clin Gastroenterol | volume = 17 | issue = 1 | pages = 75-87 | month = Feb | year = 2003 | doi =  | PMID = 12617884 }}</ref>
*Chronic pouchitis seen in approximately 15% of patients.<ref name=pmid12617884 >{{Cite journal  | last1 = Gionchetti | first1 = P. | last2 = Amadini | first2 = C. | last3 = Rizzello | first3 = F. | last4 = Venturi | first4 = A. | last5 = Poggioli | first5 = G. | last6 = Campieri | first6 = M. | title = Diagnosis and treatment of pouchitis. | journal = Best Pract Res Clin Gastroenterol | volume = 17 | issue = 1 | pages = 75-87 | month = Feb | year = 2003 | doi =  | PMID = 12617884 }}</ref>
*May be assessed by fecal calprotectin.<ref name=pmid18301296>{{Cite journal  | last1 = Johnson | first1 = MW. | last2 = Maestranzi | first2 = S. | last3 = Duffy | first3 = AM. | last4 = Dewar | first4 = DH. | last5 = Forbes | first5 = A. | last6 = Bjarnason | first6 = I. | last7 = Sherwood | first7 = RA. | last8 = Ciclitira | first8 = P. | last9 = Nicholls | first9 = JR. | title = Faecal calprotectin: a noninvasive diagnostic tool and marker of severity in pouchitis. | journal = Eur J Gastroenterol Hepatol | volume = 20 | issue = 3 | pages = 174-9 | month = Mar | year = 2008 | doi = 10.1097/MEG.0b013e3282f1c9a7 | PMID = 18301296 }}</ref>
*May be assessed by [[fecal calprotectin]].<ref name=pmid18301296>{{Cite journal  | last1 = Johnson | first1 = MW. | last2 = Maestranzi | first2 = S. | last3 = Duffy | first3 = AM. | last4 = Dewar | first4 = DH. | last5 = Forbes | first5 = A. | last6 = Bjarnason | first6 = I. | last7 = Sherwood | first7 = RA. | last8 = Ciclitira | first8 = P. | last9 = Nicholls | first9 = JR. | title = Faecal calprotectin: a noninvasive diagnostic tool and marker of severity in pouchitis. | journal = Eur J Gastroenterol Hepatol | volume = 20 | issue = 3 | pages = 174-9 | month = Mar | year = 2008 | doi = 10.1097/MEG.0b013e3282f1c9a7 | PMID = 18301296 }}</ref>
*Considered a clinico-pathologic diagnosis.<ref name=pmid20958905>{{Cite journal  | last1 = Royston | first1 = DJ. | last2 = Warren | first2 = BF. | title = Are we reporting ileal pouch biopsies correctly? | journal = Colorectal Dis | volume = 13 | issue = 11 | pages = 1285-9 | month = Nov | year = 2011 | doi = 10.1111/j.1463-1318.2010.02452.x | PMID = 20958905 }}</ref><ref name=pmid12617884 >{{Cite journal  | last1 = Gionchetti | first1 = P. | last2 = Amadini | first2 = C. | last3 = Rizzello | first3 = F. | last4 = Venturi | first4 = A. | last5 = Poggioli | first5 = G. | last6 = Campieri | first6 = M. | title = Diagnosis and treatment of pouchitis. | journal = Best Pract Res Clin Gastroenterol | volume = 17 | issue = 1 | pages = 75-87 | month = Feb | year = 2003 | doi =  | PMID = 12617884 }}</ref>
*Considered a clinico-pathologic diagnosis.<ref name=pmid20958905>{{Cite journal  | last1 = Royston | first1 = DJ. | last2 = Warren | first2 = BF. | title = Are we reporting ileal pouch biopsies correctly? | journal = Colorectal Dis | volume = 13 | issue = 11 | pages = 1285-9 | month = Nov | year = 2011 | doi = 10.1111/j.1463-1318.2010.02452.x | PMID = 20958905 }}</ref><ref name=pmid12617884 >{{Cite journal  | last1 = Gionchetti | first1 = P. | last2 = Amadini | first2 = C. | last3 = Rizzello | first3 = F. | last4 = Venturi | first4 = A. | last5 = Poggioli | first5 = G. | last6 = Campieri | first6 = M. | title = Diagnosis and treatment of pouchitis. | journal = Best Pract Res Clin Gastroenterol | volume = 17 | issue = 1 | pages = 75-87 | month = Feb | year = 2003 | doi =  | PMID = 12617884 }}</ref>
===Microscopic===
===Microscopic===
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===Sign out===
===Sign out===
Note:
*Dr. Robert Riddell is of the opinion: "Do '''not''' call any pouch inflammation as consistent with Crohn's disease."
<pre>
<pre>
SMALL BOWEL POUCH, BIOPSY:
SMALL BOWEL POUCH, BIOPSY:

Latest revision as of 17:09, 16 February 2019

Inflammatory bowel disease, abbreviated IBD, is the bread 'n butter of gastroenterology, and a significant number of gastrointestinal pathology specimens.

It exists in two main flavours:

  • Crohn's disease (CD).
  • Ulcerative colitis (UC).

Both are associated with an increased risk of colorectal carcinoma.[1]

Clinical

  • It is important to differentiate UC and CD as the management is different.
  • UC patients get pouches... CD patients do not.
    • It is said that: There 's nothing like a pouch to bring out Crohn's disease.[2]
  • People with long standing IBD have an increased risk for:

Extra-intestinal manifestations of inflammatory bowel disease

Mnemonic (family-rated version) excellent cardiac surgery is pleasant and appreciated:

Molecular

  • NOD2[5] (AKA CARD15) variants are associated with stricturing CD, early need for surgery and recurrence.[6]

General clinical differential diagnosis

  • Crohn's disease.
  • Ulcerative colitis.
  • Infective colitis/enteritis.
  • Ischemic colitis/enteritis.
  • Radiation colitis.

Others:

Specimens

  • Biopsies for diagnosis.
  • Surveillance biopsies - to rule-out dysplasia.
  • Resections for disease that has failed medical management.
  • Resections for dysplasia associated with inflammatory bowel disease.

Notes:

  • Biopsies for diagnosis should specify the (anatomical) site:
    • Slight gradients exist in the large bowel that can be exploited for diagnostic purposes if the site information is known, for example:
      • Paneth cells distal to the splenic flexure are abnormal.
      • Ulcerative colitis is often more severe distally - even in a pancolitis, as the disease starts in the rectum and progresses toward the cecum.
  • Surveillance biopsies should specify the (anatomical) site - so, it possible to find any site of interest on a follow-up colonoscopy.[7]

Biopsies all submitted in one bottle

COLON (SITE NOT FURTHER SPECIFIED), BIOPSIES:
- MODERATE CHRONIC ACTIVE COLITIS.
- NEGATIVE FOR DYSPLASIA.
- PLEASE SEE COMMENT.

COMMENT:
The sections show colorectal-type mucosa with focal cryptitis and rare neutrophilic crypt
abscesses.  

Mild architectural changes, suggestive of a chronic colitis, are present. No granulomas are
identified. Lymphoid aggregates with germinal centre formation are present in multiple
fragments.  The lamina propria has abundant plasma cells throughout the fragments; no 
fragments have apparent relative sparing. 

Paneth cells are present focally; however, the significance of the paneth cells cannot 
determined as the biopsy sites are not known. 

The findings are compatible with inflammatory bowel disease and chronic active infectious
colitides. Clinical correlation is suggested.

Microscopic

Features helpful for the diagnosis of IBD - as based on a study:[8]

  1. Basal inflammation, i.e. crypt base, plasmacytosis with severe chronic inflammation.
    • Basal cell plasmacytosis makes an infectious etiology less likely.[9]
    • "Basal plasmacytosis" = plasma cells in the lamina propria between the crypts and muscularis mucosae.[10]
  2. Crypt architectural abnormalities.
    • Atrophy = less glands ~ 3-4 glands/mm (normal = 7-8 glands/mm).
    • Branching = common (normal = very rare branching).
    • Distortion = bent glands, marked size variation[11] (normal = "rack of test tubes").
  3. Distal Paneth cell metaplasia.
    • Paneth cells should not be in the left colon[12] - if you see 'em think of IBD and other long-standing injurious processes.
    • Paneth cells have basal nuclei and coarse luminal granules.[13]
      • They should not be confused with endocrine cells -- these have apical nuclei and fine granules.
      • They should not be confused with intraepithelial eosinophils -- have smaller (~1/2) more intensely red granules.

Notes:

  1. Microscopic features can be remembered by mnemonic CPP: Crypts (abnormal), Plasmacytosis, Paneth cells where they don't belong.
  2. If you see architectural distortion (e.g. crypt branching) in the left colon, look for Paneth cells.
  3. The hepatic flexure is considered the divider for normal paneth cells and abnormal paneth cells, i.e. paneth cells proximal to the hepatic flexure are normal; paneth cells distal to the hepatic flexure are abnormal.[14]
  4. Stretching of tissue may mimic atrophy; tip-off it is artifact: thinning of mucosa.[9]

Images

Grading

  • Several systems exists.[9]
  • One that is often cited is by Gupta et al.[15]

Grading schemes for IBD in a table

Nil Mild Moderate Severe
"A grading scheme"[9] - cryptitis crypt abscesses erosions
Gupta[15] "0" (nil) "1" (<50% of crypts
have PMNs)
"2" (>50% of crypts
have PMNs)
"3" (presence of
ulcers or erosions)
Images

Crohn's disease versus ulcerative colitis

Robbins

UC features:[16]

  • Mucosal involvement -- sometimes submucosa.
  • No skip lesions.
  • Colon/rectum only.
    • UC may have 'ileal backwash' -- mild ileal inflammation due to backwash of inflammatory soup from colon.
  • "No granulomas".
    • Superficial granulomas in the mucosa are non-specific, especially if they are beside an inflamed crypt, i.e. they may be present in UC.[17][18]
      • Deep granulomas are specific for Crohn's disease.

Example of a superficial granuloma that is non-specific, i.e. this could be UC or CD:

Kirsch

Features of UC[9] - memory device DDDR:

  • Diffuse inflammation.
  • Diffuse arch. changes.
  • Diffuse atrophy.
  • Rectal involvement.

Words of caution

The following may be present in UC:[9]

  • Cecal patch (cecal involvement without pancolitis).
  • Patchy involvement
    • Esp. in Tx'ed patients.
    • Esp. in children.
  • Ileitis - esp. in the context of severe pancolitis; known as backwash ileitis.
  • Deep inflammation (in a fissure).
  • Upper GI tract involvement -- see below.

Upper gastrointestinal tract involvement

  • The old dogma was upper GI tract = Crohn's disease.

Characteristics of upper GI tract UC:[19]

  • Most common:
    1. Focal gastritis.
    2. Mixed basal inflammation and superficial plasmacytosis in the stomach.
  • Unique:
    • Diffuse chronic duodenitis.
    • ~ 10% of UC patients.
    • ~ 40% of UC + colectomy + pouchitis.

Another study compares UC, CD and control individuals:[20]

  • Gastritis:
    • UC: 19%.
    • CD: 33%
    • Controls: 13%.
  • Duodenitis:
    • UC: 3%.
    • CD: 26%.
    • Controls: 1%.

Note:

  • Younger individuals (<18 years old) have significantly more gastritis and duodenitis.[20]

A tabular comparison

Gross pathology:

Feature Crohn's disease Ulcerative colitis
Lesion distribution patchy diffuse
Strictures maybe no
Perianal disease yes/no no
Rectal involvement no yes
Ileal involvement yes, classic usu. no; seen in pancolitis
Upper GI tract involvement yes yes (gaining acceptance)
Associated with PSC not classically yes

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Quiescent inflammatory bowel disease

  • No accepted formal definition.

May be used when:

  1. Non-specific "minimal abnormalities" are present.
  2. There is a history of inflammatory bowel disease.

"Minimal abnormalities" - features:

  • Apoptosis.
  • Macrophages in the lamina propria.
  • Lymphoid nodules.
  • "Abundant" plasma cells in the lamina propria.
    • Abundant is subjective.
COLON, BIOPSIES:
- QUIESCENT INFLAMMATORY BOWEL DISEASE.
- NEGATIVE FOR DYSPLASIA.

Mild inflammation

SIGMOID COLON, BIOPSY:
- MILD ACTIVE COLITIS WITH CHRONIC CHANGES, SEE COMMENT.
- NEGATIVE FOR DYSPLASIA.

COMMENT:
No granulomata are identified.  Mild architectural changes are present.

The findings are compatible with inflammatory bowel disease or an infectious
etiology. Clinical correlation is required.

Mild-to-moderate inflammation

COLON, LEFT, BIOPSY:
- MILD-TO-MODERATE ACTIVE COLITIS WITH CHRONIC CHANGES.
- NEGATIVE FOR DYSPLASIA.

COMMENT:
No definite granulomata are identified.  Mild architectural changes are present.
Cryptitis is seen in several crypts. Rare crypt abscesses are present. Lamina propria
plasma cells are abundant throughout the biopsy.

The findings are compatible with inflammatory bowel disease or an infectious
etiology. Clinical correlation is required.

Moderate inflammation

RECTUM, BIOPSY:
- RECTAL MUCOSA WITH MODERATE ACTIVE INFLAMMATION AND CHRONIC CHANGES.
- NEGATIVE FOR DYSPLASIA.
- SEE COMMENT.

COMMENT:
No definite granulomata are identified. Architectural changes, including crypt drop out,
are present. Lamina propria plasma cells are abundant throughout the biopsy and eosinophil 
numbers are mildly increased. Lymphoid aggregates with germinal centre formation are 
present. All fragments of tissue are affected.

The findings are compatible with inflammatory bowel disease or an infectious
etiology. Clinical correlation is required.

Specific diagnoses

Ulcerative colitis

  • Often abbreviated as UC.

Crohn's disease

  • Abbreviated CD.

"Indeterminate colitis"

  • "Indeterminate colitis" is a confusing term and should be avoided.[21]

Suggested terminology

  1. IBDU = IBD unclassified.
  2. CUTE = Colitis of uncertain type or etiology.
    • Should be reserved for resection specimens only.

Dysplasia in inflammatory bowel disease

General

Classified as per Riddell et al.:[22][23][24]

Notes:

  • GI experts and generalists have similar rates of agreement.[23]

Microscopic

Features:[25]

  • Nuclear changes at the surface - key feature.
    • Nuclear hyperchromasia.
    • Nuclear enlargement - ellipsoid or spherical.

Dysplasia-associated lesion or mass

  • Abbreviated DALM.

Pouchitis

General

  • Inflammation of an ileal pouch; pouches are a treatment for ulcerative colitis.
    • Generally, pouches are not used in Crohn's disease.
  • Chronic pouchitis seen in approximately 15% of patients.[26]
  • May be assessed by fecal calprotectin.[27]
  • Considered a clinico-pathologic diagnosis.[28][26]

Microscopic

Features:[29]

Note:

  • Absence of Paneth cells and villi = colonic metaplasia,[30] associated with inflammation.[31]

DDx:

Images:

Scoring system

Pouchitis disease activity index (PDAI) - based on clinical and pathologic factors:

  • Active pouchitis >= 7.
  • Remission < 7.

The histologic component of the PDAI:[29]

  • Neutrophils.
    • Mild.
    • Moderate - crypt abscesses.
    • Severe - crypt abscesses.
  • Ulceration per LPF (mean).
    • <25%.
    • 25-50%.
    • >50.

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Note:

  • Dr. Robert Riddell is of the opinion: "Do not call any pouch inflammation as consistent with Crohn's disease."
SMALL BOWEL POUCH, BIOPSY:
- SMALL BOWEL MUCOSA WITH CHRONIC ACTIVE INFLAMMATION WITH ULCERATION, EARLY
  CRYPT ABSCESS FORMATION, CRYPTITIS, AND LOSS OF THE VILLOUS ARCHITECTURE.
- NEGATIVE FOR GRANULOMAS AND NEGATIVE FOR PYLORIC GLAND METAPLASIA.
- NEGATIVE FOR DYSPLASIA.

COMMENT:
The findings are consistent with pouchitis.

Pyloric gland metaplasia present

SMALL BOWEL POUCH, BIOPSY:
- SMALL BOWEL MUCOSA WITH CHRONIC ACTIVE INFLAMMATION WITH ULCERATION, EARLY
  CRYPT ABSCESS FORMATION, CRYPTITIS, AND LOSS OF THE VILLOUS ARCHITECTURE.
- PYLORIC GLAND METAPLASIA, FOCAL, SEE COMMENT.
- NEGATIVE FOR GRANULOMAS.
- NEGATIVE FOR DYSPLASIA.

COMMENT:
The presence of pyloric gland metaplasia raises the possibility of Crohn's disease;
however, in the context of previous biopsies with inflammation, the concurrent
negative ileal biopsy and lack of granulomas, this individual is favoured to have 
pouchitis.

See also

References

  1. Schmidt C, Bielecki C, Felber J, Stallmach A (June 2010). "Surveillance strategies in inflammatory bowel disease". Minerva Gastroenterol Dietol 56 (2): 189–201. PMID 20485256.
  2. URL: http://www.gihealthfoundation.org/library/ppts/postcolectomypatient.pdf. 3 March 2011.
  3. 3.0 3.1 Claessen, MM.; Siersema, PD.; Vleggaar, FP. (Apr 2011). "IBD-related carcinoma.". Best Pract Res Clin Gastroenterol 25 Suppl 1: S27-38. doi:10.1016/S1521-6918(11)70007-5. PMID 21640928.
  4. Vos, AC.; Bakkal, N.; Minnee, RC.; Casparie, MK.; de Jong, DJ.; Dijkstra, G.; Stokkers, P.; van Bodegraven, AA. et al. (Sep 2011). "Risk of malignant lymphoma in patients with inflammatory bowel diseases: A Dutch nationwide study.". Inflamm Bowel Dis 17 (9): 1837-1845. doi:10.1002/ibd.21582. PMID 21830262.
  5. Online 'Mendelian Inheritance in Man' (OMIM) 605956
  6. Alvarez-Lobos M, Arostegui JI, Sans M, et al. (November 2005). "Crohn's disease patients carrying Nod2/CARD15 gene variants have an increased and early need for first surgery due to stricturing disease and higher rate of surgical recurrence". Ann. Surg. 242 (5): 693–700. PMC 1409853. PMID 16244543. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1409853/.
  7. Panaccione, R. (Apr 2006). "The approach to dysplasia surveillance in inflammatory bowel disease.". Can J Gastroenterol 20 (4): 251-3. PMC 2659899. PMID 16609751. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2659899/.
  8. Tanaka M, Riddell RH, Saito H, Soma Y, Hidaka H, Kudo H (January 1999). "Morphologic criteria applicable to biopsy specimens for effective distinction of inflammatory bowel disease from other forms of colitis and of Crohn's disease from ulcerative colitis". Scand. J. Gastroenterol. 34 (1): 55–67. PMID 10048734.
  9. 9.0 9.1 9.2 9.3 9.4 9.5 Kirsch, R. 13 December 2010.
  10. "Pathology of ulcerative colitis". http://www.histopathology-india.net/UlCol.htm. Retrieved 17 January 2011.
  11. URL: http://www.histopath.com.au/assets/documents/Inflammatory%20bowel%20disease.pdf. Accessed on: 25 October 2013.
  12. Tanaka M, Saito H, Kusumi T, et al (December 2001). "Spatial distribution and histogenesis of colorectal Paneth cell metaplasia in idiopathic inflammatory bowel disease". J. Gastroenterol. Hepatol. 16 (12): 1353–9. PMID 11851832. http://www3.interscience.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0815-9319&date=2001&volume=16&issue=12&spage=1353.
  13. Mills, Stacey E. (2006). Histology for Pathologists (3rd ed.). Lippincott Williams & Wilkins. pp. 631. ISBN 9780781762410.
  14. STC. 14 December 2009.
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  16. Cotran, Ramzi S.; Kumar, Vinay; Fausto, Nelson; Nelso Fausto; Robbins, Stanley L.; Abbas, Abul K. (2005). Robbins and Cotran pathologic basis of disease (7th ed.). St. Louis, Mo: Elsevier Saunders. pp. 850. ISBN 0-7216-0187-1.
  17. Shepherd, NA. (Aug 2002). "Granulomas in the diagnosis of intestinal Crohn's disease: a myth exploded?". Histopathology 41 (2): 166-8. PMID 12147095.
  18. Mahadeva, U.; Martin, JP.; Patel, NK.; Price, AB. (Jul 2002). "Granulomatous ulcerative colitis: a re-appraisal of the mucosal granuloma in the distinction of Crohn's disease from ulcerative colitis.". Histopathology 41 (1): 50-5. PMID 12121237.
  19. Lin J, McKenna BJ, Appelman HD (November 2010). "Morphologic findings in upper gastrointestinal biopsies of patients with ulcerative colitis: a controlled study". Am. J. Surg. Pathol. 34 (11): 1672–7. doi:10.1097/PAS.0b013e3181f3de93. PMID 20962621.
  20. 20.0 20.1 Sonnenberg, A.; Melton, SD.; Genta, RM. (Jan 2011). "Frequent occurrence of gastritis and duodenitis in patients with inflammatory bowel disease.". Inflamm Bowel Dis 17 (1): 39-44. doi:10.1002/ibd.21356. PMID 20848539.
  21. Geboes K, Colombel JF, Greenstein A, et al. (June 2008). "Indeterminate colitis: a review of the concept--what's in a name?". Inflamm. Bowel Dis. 14 (6): 850–7. doi:10.1002/ibd.20361. PMID 18213696.
  22. Riddell, RH.; Goldman, H.; Ransohoff, DF.; Appelman, HD.; Fenoglio, CM.; Haggitt, RC.; Ahren, C.; Correa, P. et al. (Nov 1983). "Dysplasia in inflammatory bowel disease: standardized classification with provisional clinical applications.". Hum Pathol 14 (11): 931-68. PMID 6629368.
  23. 23.0 23.1 Eaden, J.; Abrams, K.; McKay, H.; Denley, H.; Mayberry, J. (Jun 2001). "Inter-observer variation between general and specialist gastrointestinal pathologists when grading dysplasia in ulcerative colitis.". J Pathol 194 (2): 152-7. doi:10.1002/path.876. PMID 11400142.
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  25. URL: http://surgpathcriteria.stanford.edu/gi/ulcerative-colitis/printable.html. Accessed on: 12 March 2013.
  26. 26.0 26.1 Gionchetti, P.; Amadini, C.; Rizzello, F.; Venturi, A.; Poggioli, G.; Campieri, M. (Feb 2003). "Diagnosis and treatment of pouchitis.". Best Pract Res Clin Gastroenterol 17 (1): 75-87. PMID 12617884.
  27. Johnson, MW.; Maestranzi, S.; Duffy, AM.; Dewar, DH.; Forbes, A.; Bjarnason, I.; Sherwood, RA.; Ciclitira, P. et al. (Mar 2008). "Faecal calprotectin: a noninvasive diagnostic tool and marker of severity in pouchitis.". Eur J Gastroenterol Hepatol 20 (3): 174-9. doi:10.1097/MEG.0b013e3282f1c9a7. PMID 18301296.
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  29. 29.0 29.1 Shen, B.; Achkar, JP.; Connor, JT.; Ormsby, AH.; Remzi, FH.; Bevins, CL.; Brzezinski, A.; Bambrick, ML. et al. (Jun 2003). "Modified pouchitis disease activity index: a simplified approach to the diagnosis of pouchitis.". Dis Colon Rectum 46 (6): 748-53. doi:10.1097/01.DCR.0000070528.00563.D9. PMID 12794576.
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  35. Shen, B.; Liu, W.; Remzi, FH.; Shao, Z.; Lu, H.; DeLaMotte, C.; Hammel, J.; Queener, E. et al. (Sep 2008). "Enterochromaffin cell hyperplasia in irritable pouch syndrome.". Am J Gastroenterol 103 (9): 2293-300. doi:10.1111/j.1572-0241.2008.01990.x. PMID 18702649.

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