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Difference between revisions of "Stains"
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[[Image:Corpora amylacea high mag.jpg|thumb|300px|[[Hematoxylin and eosin stain]] of benign [[prostate gland]].]] | [[Image:Corpora amylacea high mag.jpg|thumb|300px|[[Hematoxylin and eosin stain]] of benign [[prostate gland]].]] | ||
This article deals with '''stains'''. H&E | This article deals with '''stains'''. The H&E stain is only one of many stains that are used in pathology. | ||
Non-H&E stains are often referred to as '''''special stains'''''. | Non-H&E stains are often referred to as '''''special stains'''''. | ||
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There are several AFB stains: | There are several AFB stains: | ||
*Ziehl-Neelson stain - used to look for ''[[Mycobacterium tuberculosis]]''. | *Ziehl-Neelson stain - used to look for ''[[Mycobacterium tuberculosis]]''. | ||
*Fite stain - used to look for ''[[Mycobacterium leprae]]'' | *Fite stain - used to look for ''[[Mycobacterium leprae]]''<ref>URL: [http://library.med.utah.edu/WebPath/HISTHTML/MANUALS/FITES.PDF http://library.med.utah.edu/WebPath/HISTHTML/MANUALS/FITES.PDF]. Accessed on: 19 May 2011.</ref> and preferred stain for ''[[Mycobacterium avium complex]]''.<ref name=pmid21327589>{{cite journal |authors=Ilyas S, Youssef D, Chaudhary H, Al-Abbadi MA |title=Myocbacterium-avium intracellulare associated inflammatory pseudotumor of the anterior nasal cavity |journal=Head Neck Pathol |volume=5 |issue=3 |pages=296–301 |date=September 2011 |pmid=21327589 |pmc=3173547 |doi=10.1007/s12105-011-0248-0 |url=}}</ref> | ||
*Auramine-rhodamine stain. | *Auramine-rhodamine stain. | ||
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*Red = AFB. | *Red = AFB. | ||
*Blue = background. | *Blue = background. | ||
===Auramine-rhodamine stain=== | ===Auramine-rhodamine stain=== | ||
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*Red = muscle (smooth muscle actin). | *Red = muscle (smooth muscle actin). | ||
*Baby blue = collagen. | *Baby blue = collagen. | ||
*Blue gray = [[amyloid]].<ref name=pmid33002919>{{cite journal |authors=Kunnath-Velayudhan S, Larsen BT, Coley SM, De Michele S, Santoriello D, Colby TV, Bhagat G, Saqi A |title=Masson Trichrome and Sulfated Alcian Blue Stains Distinguish Light Chain Deposition Disease From Amyloidosis in the Lung |journal=Am J Surg Pathol |volume=45 |issue=3 |pages=405–413 |date=March 2021 |pmid=33002919 |doi=10.1097/PAS.0000000000001593 |url=}}</ref> | |||
Notes: <Ref>ULR: [http://library.med.utah.edu/WebPath/HISTHTML/MANUALS/MASSONS.PDF http://library.med.utah.edu/WebPath/HISTHTML/MANUALS/MASSONS.PDF]. Accessed on: 2 November 2011.</ref> | Notes: <Ref>ULR: [http://library.med.utah.edu/WebPath/HISTHTML/MANUALS/MASSONS.PDF http://library.med.utah.edu/WebPath/HISTHTML/MANUALS/MASSONS.PDF]. Accessed on: 2 November 2011.</ref> | ||
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==Hale's colloidal iron stain== | ==Hale's colloidal iron stain== | ||
{{ | {{Main|Hale's colloidal iron stain}} | ||
==von Kossa stain== | ==von Kossa stain== | ||
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Image: | Image: | ||
*[ | *[https://www.ole.bris.ac.uk/bbcswebdav/institution/Faculty%20of%20Health%20Sciences/Veterinary%20Science/eLearning%20resources/Pathology%20Laboratory%20Protocols/hst/msb.html MSB (bris.ac.uk)]. | ||
Ref.:<ref>URL: [http://www.bris.ac.uk/vetpath/cpl/msb.html http://www.bris.ac.uk/vetpath/cpl/msb.html]. Accessed on: 26 November 2010.</ref> | Ref.:<ref>URL: [http://www.bris.ac.uk/vetpath/cpl/msb.html http://www.bris.ac.uk/vetpath/cpl/msb.html]. Accessed on: 26 November 2010.</ref> | ||