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Difference between revisions of "Molecular pathology"
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→Karyotyping
(→Karyotyping: more) |
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====Karyotyping==== | ====Karyotyping==== | ||
What? | |||
*Metaphase nuclei. | *Metaphase nuclei. | ||
How? | |||
*Chromosomes are identified by: | *Chromosomes are identified by: | ||
**Size (chromosome 1 = largest, chromosome 22 = smallest). | **Size (chromosome 1 = largest, chromosome 22 = smallest). | ||
**Position of centromere. | **Position of centromere. | ||
**Banding pattern - | **Banding pattern - using (special) stains: | ||
***Several different techniques (stains) are used:<ref>URL: [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC355922/ http://www.ncbi.nlm.nih.gov/pmc/articles/PMC355922/]. Accessed on: 10 May 2011.</ref> | ***Several different techniques (stains) are used:<ref>URL: [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC355922/ http://www.ncbi.nlm.nih.gov/pmc/articles/PMC355922/]. Accessed on: 10 May 2011.</ref> | ||
****Examples: C-banding (C=constitutive heterchromatin), G-banding (G=Giemsa | ****Examples: Q-banding (Q=Quinacrine), C-banding (C=constitutive heterchromatin), G-banding (G=Giemsa), R-banding (R=reverse). | ||
*****Q-banding is the standard at UHN. | |||
Image: | Image: | ||
*[http://commons.wikimedia.org/wiki/File:NHGRI_human_male_karyotype.png Karyotype (WC)]. | *[http://commons.wikimedia.org/wiki/File:NHGRI_human_male_karyotype.png Karyotype (WC)]. | ||
Notes: | |||
*Quinacrine dye - AT-rich regions brighter than GC-rich regions. | |||
====ISH==== | ====ISH==== | ||