Difference between revisions of "Molecular pathology"

Molecular pathology (view source)

Revision as of 14:26, 10 May 2011

1,620 bytes removed , 14:26, 10 May 2011

split-out cytogenetics

Michael

48,830

edits

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 {{familytree | | | |A11| | | | |A11 =Molecular<br>pathology}}
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-{{familytree | B11 | | | | B12 |B11=Molecular<br>techniques|B12=Cytogenetics}}
+{{familytree | B11 | | | | B12 |B11=Molecular<br>techniques|B12=[[Cytogenetics]]}}
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 ==Cytogenetics==
-===General===
+{{Main|Cytogenetics}}
-*Large changes (chromosomal).
+This deals with karyotyping and ISH.
-**Maximum resolution 3-4 megabase pairs (3-4 million base pairs); may be less - dependent on band density.<ref>{{Ref WMSP|695}}</ref>
-*Morphologic data.
-===Techniques===
-*ISH = in situ hybridization.
-**FISH = fluorescent in situ hybridization.
-**SISH = silver in situ hybridization.<ref>URL: [http://www.immunoportal.com/modules.php?name=News&file=article&sid=186 http://www.immunoportal.com/modules.php?name=News&file=article&sid=186]. Accessed on: 2 May 2011.</ref>
-*Karyotyping.
-====Karyotyping====
-What?
-*Metaphase nuclei.
-How?
-*Chromosomes are identified by:
-**Size (chromosome 1 = largest, chromosome 22 = smallest).
-**Position of centromere.
-**Banding pattern - using (special) stains:
-***Several different techniques (stains) are used:<ref>URL: [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC355922/ http://www.ncbi.nlm.nih.gov/pmc/articles/PMC355922/]. Accessed on: 10 May 2011.</ref>
-****Examples: Q-banding (Q=Quinacrine), C-banding (C=constitutive heterchromatin), G-banding (G=Giemsa), R-banding (R=reverse).
-*****Q-banding is the standard at UHN.
-Image:
-*[http://commons.wikimedia.org/wiki/File:NHGRI_human_male_karyotype.png Karyotype (WC)].
-Notes:
-*Quinacrine dye - AT-rich regions brighter than GC-rich regions.
-====ISH====
-*Usu. interphase nuclei.
-May be prepared from:
-*In situ paraffin sections (section 4 micrometers thick).
-*Isolated nuclei (section 10 micrometers thick).
-*Cytospin.
-*Cultured cells.
-=====Probe types=====
-Types:
-*Fusion (two colours).
-*Breakapart (two colours).
-*Deletion/duplication (e.g. trisomy).
-Image:
-*[http://commons.wikimedia.org/wiki/File:Bcrablmet.jpg Bcr-Abl translocation (WC)].
 ==Other garbage==

Difference between revisions of "Molecular pathology"

Molecular pathology (view source)

Revision as of 14:26, 10 May 2011

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