Difference between revisions of "Forensic pathology"

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*Grade 3: macroscopic and microscopic findings.
*Grade 3: macroscopic and microscopic findings.


IHC: beta-APP.<ref>http://www.ncbi.nlm.nih.gov/pubmed/10050789</ref>
IHC: beta-amyloid precursor protein (beta-APP ''or'' APP).<ref name=pmid10050789>{{cite journal |author=Gleckman AM, Bell MD, Evans RJ, Smith TW |title=Diffuse axonal injury in infants with nonaccidental craniocerebral trauma: enhanced detection by beta-amyloid precursor protein immunohistochemical staining |journal=Arch. Pathol. Lab. Med. |volume=123 |issue=2 |pages=146–51 |year=1999 |month=February |pmid=10050789 |doi= |url=}}</ref>


===Intracranial hemorrhage===
===Intracranial hemorrhage===

Revision as of 19:22, 9 September 2010

Forensic pathology is figuring-out why people died... along with when and how (if possible).

Manner of death

 
 
 
 
 
 
 
 
Manner
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
Homicide
 
Suicide
 
Natural
 
Accident
 
Undetermined

Notes:

  • Undetermined - is a waste basket category.
  • Homicide - not necessarily murder.
  • Can be group into three:
    1. Intent to kill (homicide, suicide).
    2. No intent to kill (natural, accidental).
    3. Undetermined.

Cause of death

General

  • The cause of death should be what started the sequence of events that lead to death.

Example:

  • C. difficle colitis complicating antibiotic treatment for a dental abscess.[1]
  • Complications of laparoscopic cholecystectomy for ascending choleangitis with mesothelioma and atherosclerotic heart disease.[2]

General forms:

  • A complicating B for the treatment of C.
  • A complicating B for the treatment of C with D and E.

Natural deaths

  • The cause should be a medical diagnosis, not the mechanism (e.g. cardiac arrest, cachexia, kidney failure).
  • The mechanism is irrelevant.

Notes:

Legal frame work

  • In Ontario, the manner is determined by the coroner.
  • Coroners, in Ontario, are MDs -- usually family docs.
  • The cause (e.g. "gunshot wound to the head") is determined by the pathologist.

NB - the word coroner is not synoymous with MD. British Columbia has coroners that aren't MDs.

Forensic triangle

Most general differential diagnosis:

  • Natural:
    • Haemorrhage (e.g. cerebral bleed, gastrointestinal bleed, aortic aneurysm).
    • Infection (e.g. pneumonia).
    • Coronary atherosclerosis (cardiac arrhythmias - more common in the forensic context than myocardial infarction (MI); individuals with MIs don't usu. drop dead-- they go to the ER).
      • Post myocardial infarction (free wall rupture).
      • Ruptured (atherosclerotic) plaque.
  • Toxic (memory device: PAIRO):
    • Poisons.
    • Alcohol (EtOH).
    • Illicit (e.g. cocaine, heroin, LSD).
    • Rx.
    • Over-the-counter (OTC) (e.g. acetaminophen, warfarin).
  • Trauma (memory device AGE BS):
    • Asphyxial.
    • Gunshot wound (GSW).
    • Environmental (e.g. hypothermia, hyperthermia, drowning, lack of oxygen).
    • Blunt force trauma.
    • Sharp force trauma.

Difficulties arise when more than one point of the triangle is in play, i.e. the forensic pathologist has to earn their pay when an old man with a heart condition is known to be into erotic asphyxia, and dies after doing some drugs and whilst indulging in erotic asyphxiation with a friend...

  • If he had an arrhythmia and there was no stressor... natural death.
  • If he over did it with the drugs, it is an overdose, ergo accidental.
  • If he did the erotic asphyxia a bit too long it is accidental.
  • If the friend held the plastic bag over his head just a bit long to asphyxiate him... it is a homicide.
  • If he was a lone and depressed... he might have been trying to kill himself, ergo suicide.

Death-related changes

Rigor mortis

Def'n: muscle rigidity following death (caused by depletion of ATP).

Dependent on:

  • Temperature of patient at death.
  • Temperature variations in the environment since death.
  • Presence of some medical conditions.
  • May never develop!

Summary: Its onset & presence is highly variable. Therefore, it is only marginally useful for determining the time of death.

A crude guess for time of death based on rigor:[4]

  • Warm & flaccid <3 h.
  • Warm & stiff 3-8 h.
  • Cold & stiff 8-36 h.
  • Cold & flaccid > 36 h.

Notes:

  • Memory device: 3s: cut points are at 3 hours, 1/3 of a day, 3/2 of a day.

Livor mortis

Def'n: pooling of blood in the dependent position, due to blood stasis.

  • Onset may preceed death in the context of congestive heart failure.
  • If pressure is applied to a dependent area-- no blood can enter there; thus, a pressure area is blanched (i.e. white).
  • Can be seen externally, i.e. on the skin, and internally.
  • Liver mortis becomes fixed some time after death.
    • Liver mortis does NOT tell one the position the decedent was in at the time of death-- only the position the decedent was at the time liver mortis became fixed. If the decedent wasn't moved liver mortis can help determine the position the person was in when they died.

Decomposition

According to textbooks one of two things happens post-mortem:

  • Mummification, or
  • Putrefaction.

Real life:

  • A mix of mummification and putrefaction occur, i.e. part of the corpse is mummified... part of it decomposed through putrefaction.

Mummification:

  • Predominant in dry environments.
  • Body becomes dry and leathery.

Putrefaction:

  • Body wet/moist after death -- ideal environment for putrefactive bacteria and organisms.

Artefacts

  • Prinsloo and Gordon artefact = artefactual post-morten haemorrhage on the posterior surface of the esophagus.[5]

Classification of wounds

Mnemonic CALI:

  • Contusion - "bruise", haemotoma
  • Abrasion - "scrape", e.g. motorcyclist slide across the roadway... skin ripped-off
  • Laceration - "tear", trauma distant from where skin split
  • Incised - "cut", e.g. caused by a knife.[6]
    1. "Cut" or "slash" = length > depth.
    2. "Stab" = depth > length.

How to decide what you're looking at:

  • Contusion:
    • Can be demonstrated histologically... there are extravascular RBCs.
      • If pre-morten there is vital reaction, i.e. WBCs come to clean-up the trauma.
    • If the post mortem interval is not known and long-- differentiation from decomposition may be non-trivial/impossible.
  • Abrasion vs. contusion:
    • Contusions skin is intact... in abrasion it is not.
    • Abrasions and contusions may be co-localized, i.e. in the same place.
  • Laceration vs. incision:
    • Lacerations have "bridges", incisions do NOT have bridges.
      • Bridges are fine strands of tissue that cross the long axis of the skin defect.
        • You can think of the wound as partially "sutured" by the bridges of tissue.

Autopsy

Main article: Autopsy

The autopsy article covers procedural things. Heart dissection is covered in the heart article.

Types

Forensic vs. hospital:

  • Forensic autopsies are focused on the external exam.

Marking conventions for common findings

There are no universal marking conventions for injuries.

One system in use (the Rose system) is:[7]

  • One red line for an incised wound.
  • Multiple closely spaced red lines, i.e. red hatching, for abrasions.
  • Multiple closely-spaced blue lines, i.e. blue hatching, for contusions.

The above makes sense in that:

  • Abrasions and incised wounds typically bleed - are red.
  • Contusions (bruises) don't classically bleed and are classically blue.

External exam findings

Colour of the corpse:[8]

  • Red (Pink) = carbon monoxide, cyanide, fluoroacetate.[9]
  • Purple (intense) = propane.
  • Green = hydrogen sulfide.
  • Brown = nitrites (methemoglobinemia).

Autopsy terminology

  • Gutter butter = adipose tissue in a decomp case; looks like butter topping put on popcorn. A Toronto-ism.
  • Gutter blood = blood in the empty thorax - after extraction of the organ block.
  • Tardieu spots = postmortem hypostatic hemorrhages;[10] look like large petechiae - in dependent areas.

Causes of death

Hanging

Classic:

  • V-shaped furrow on the neck.
  • V "open" at the point of suspension.

Drowning

Classic:

  • Autopsy is often negative, i.e. there is no anatomical cause of death.

Drowning is difficult to prove on autopsy.[5] The diagnosis is often based on circumstance, i.e. the scene.

Macroscopic

External:

  • Plume of froth at the mouth/nostrils (fresh drowning only).[5]

Internal:

  • Lung emphysema.
  • Froth in the trachea.
  • Patlauf spots[11] -- ???

Micro/Lab

There are a few tests of debated value:[5]

  • Diffusion of particulates into the blood (left ventricle) from the water.
    • Diatom test.
  • Haemodilution.
    • Mostly useless - CPR interferes with it, not reliable if there putrefaction or autolysis.
  • Transport of aveolar element into blood circulation.

Carbon monoxide

Carbon monoxide (CO) is a common way to commit suicide.

Pathophysiology:

  • CO binds to haemoglobin -- prevents oxygen from binding there.

Findings:

  • Salmon pink skin.
  • CO level (blood test) elevated (???).

Gunshot wounds

Main article: Gunshot wounds

Gunshot wounds (GSWs) are a relatively uncommon finding in Canada. They are dealt within a separate article.

Asphyxia

  • This is a big topic.

Manual strangulation

Features:[12]

  • Petechiac of sclerae (white part of eye) & conjunctivae (cover sclerae); present ~ 90% of the time.
  • Hyoid bone fracture.
  • Thyroid cartilage fracture.
  • Haemorrhage in strap muscles of the neck.

Blunt force trauma

  • Pretty much anything.
  • Beer bottles are common... and strong enought to fracture a skull.
    • Empty bottles have a higher fracture energy than full ones.[13]

Sharp force trauma

  • Knife or other sharp object.

Aortic trauma

  • Aortic dissection due to trauma is often catastrophic.
  • Classic location of injury is subclavian branch point.[14]

Dissections often classified as:[15]

  • Type A - aortic root to just distal of left subclavian artery.
  • Type B - distal to (left) subclavian artery.

Head injuries

Diffuse axonal injury

Clinical:

  • Vegetative state. (???)
  • Imaging findings: no anatomical cause apparent in some cases. (???)

Etiology:

  • Hypothesized to arise from high shear loading of white mater tracts.[16]

Macroscopic findings:[16]

  • Tears - corpus callosum.
  • Haemorrhage.

Microscopic findings:[16]

  • Axonal retraction balls.
  • "Microglial stars".
  • Degeneration of fibre tracts.

Grading:

  • Grade 1: only microscopic findings.
  • Grade 2: ?
  • Grade 3: macroscopic and microscopic findings.

IHC: beta-amyloid precursor protein (beta-APP or APP).[17]

Intracranial hemorrhage

Main article: Intracranial hematoma

Classification:

  • Epidural hematoma.
  • Subdural hematoma.
  • Subarachnoid hematoma.

Traumatic brain injury in infants

Main article: Traumatic brain injury in infants
  • Shaken-impact syndrome.
  • Shaken baby syndrome.

Excited delirium

General:

  • Also known as "agitated delirium".[18]
  • Dx is considered controversial, especially outside of the forensic pathology community.[19]
  • The diagnosis has garnered considerable attention in the context of electroshock weapon use, as Taser International (a manufacturer of electroshock weapons) has blamed all deaths involving its weapons on it.
  • There is no "official" definition for excited delirium.
    • Most agree it includes fever.

One paper defines it in relation neuroleptic malignant syndrome:[18]

  • Fever.
  • Disorientation and confusion.
  • Increased energy/superhuman strength.

Excited delirium - hypothesis:

  • Thought to arise in the context of severe chronic mental disorders (e.g. schizophrenia) and protracted cocaine binges.[20]
  • Thought to result from alteration of dopamine receptor density. The D2 receptor in particular, which is thought to be important in temperature regulation, is decreased in psychotic cocaine abusers.[18]

Toxicology

Cocaine

  • No agreed upon toxic dose.[21] (due to tolerance)
  • Chronic use may lead to cardiac enlargement.

Ethylene glycol

  • Not done in routine toxicology screening.
  • Birefringent calcium oxalate crystals found in kidney (with polarized light).[22]

Anaphylaxis

Serology:[23]

  • IgE.
  • Tryptase.

Natural death

There is a lot that can kill ya... but only a few of those are quickly, i.e. within a hour or so.

Generally, these things are:

  • Cardiovascular:
    • Arrhythmia.
    • Myocardial infarction.
    • Haemorrhage.
      • Ruptured aneurysm.
  • Respiratory:
    • Pulmonary embolism (PE).
    • Asthma.
  • GI:
    • Haemorrhage.
      • Esophageal varices.
      • Gastric varices.
  • Neurologic:
    • Intracranial haemorrhage.
      • Ruptured aneurysm.
      • Spontaneous subdural haemorrhage.
    • Stroke:
      • Haemorrhagic.
      • Thrombotic (more common than haemorrhagic).

A list:[24] Respiratory:

  • Anaphylaxis.
  • Asthma.
  • Pulmonary embolism.

Hemorrhagic:

  • Ruptured AAA.
  • Peptic ulcer.
  • Cerebral aneurysm.

Cerebral:

  • SUDEP (sudden unexpected death in epilepsy).

Cardiac:

  • CAD.
  • Right ventricular cardiomyopathy.
  • Arrhythmia.

Detailed cardiac:

  • CAD, hypertrophic CM.
  • ARVC.
  • DCM.
  • Lymphocytic myocarditis.
  • Floppy MV.
  • Aortic valve stenosis.
  • Congenital cardiac abnormality.
  • Coronary artery dissection.
  • Aortic dissection.
  • Arrhythmia.
    • Long QT syndrome, Brugada syndrome, short QT syndrome, catecholaminergic polymorphic VT, anomalous conduction pathways, dysplasia of nodal arteries, atrioventricular node tumour.

Forensic entomology

Main article: Forensic entomology
  • Study of the bugs that eat corpses.
  • Bugs may hide a wound... it is important to know where they like to be.

Forensic anthropology

General

  • Look at bones[25] may assist in:
    • Identification - age, sex.
    • Cause of death.

Male versus female

Pelvic:[26]

  • Pelvic inlet (superior) - heart-shaped in male, round in female.
  • Pelvic outlet (inferior) - small in male, large in female.
  • Obturator foramen (shape) - round in males, oval in females.
  • Acetabulum (size) - large in males, small in females.
  • Subpubic angle - ~70 degrees in males, 90-100 degrees in females.
  • Sciatic notch (shape) - broad in females, narrow in males.[27]

Mandible:

  • Angle of mandible (shape) - square-ish in male, round in female.[27]

Skull:

  • Supraorbital ridges - more prominent in males.
  • Glabella - more prominent in males.
  • Temporal lines[28] (temporalis muscle attachment) - more prominent in males.

Notes:

  • Anthropologic grouping is not absolute, i.e. it may be wrong.
  • Femoral neck-shaft angles not discriminative.[29]

Additional references

See also

References

  1. ↑ MSP. 8 September 2010.
  2. ↑ TR. 3 September 2010.
  3. ↑ PS. 2009.
  4. ↑ Saukko, Pekka; Knight, Bernard (2004). Knight's Forensic Pathology (3rd ed.). A Hodder Arnold Publication. pp. 61. ISBN 978-0340760444.
  5. ↑ 5.0 5.1 5.2 5.3 Piette MH, De Letter EA (November 2006). "Drowning: still a difficult autopsy diagnosis". Forensic Sci. Int. 163 (1-2): 1–9. doi:10.1016/j.forsciint.2004.10.027. PMID 16378701.
  6. ↑ DiMaio, Vincent J.M.; Dana, Suzanna E. (2006). Handbook of Forensic Pathology (2nd ed.). CRC Press. pp. 154. ISBN 978-0849392870.
  7. ↑ TR. 1 September 2010.
  8. ↑ Shkrum, Michael J.; Ramsay, David A. (2006). Forensic Pathology of Trauma: Common Problems for the Pathologist (1st ed.). Humana Press. pp. 33. ISBN 978-1588294586.
  9. ↑ Proudfoot AT, Bradberry SM, Vale JA (2006). "Sodium fluoroacetate poisoning". Toxicol Rev 25 (4): 213–9. PMID 17288493.
  10. ↑ Pollanen MS, Perera SD, Clutterbuck DJ (December 2009). "Hemorrhagic lividity of the neck: controlled induction of postmortem hypostatic hemorrhages". Am J Forensic Med Pathol 30 (4): 322–6. doi:10.1097/PAF.0b013e3181c17ec2. PMID 19901802.
  11. ↑ Bohnert M, Ropohl D, Pollak S (2002). "[Forensic medicine significance of the fluid content of the sphenoid sinuses]" (in German). Arch Kriminol 209 (5-6): 158–64. PMID 12134758.
  12. ↑ DiMaio, Vincent J.M.; Dana, Suzanna E. (2006). Handbook of Forensic Pathology (2nd ed.). CRC Press. pp. 155. ISBN 978-0849392870.
  13. ↑ Bolliger SA, Ross S, Oesterhelweg L, Thali MJ, Kneubuehl BP (April 2009). "Are full or empty beer bottles sturdier and does their fracture-threshold suffice to break the human skull?". J Forensic Leg Med 16 (3): 138–42. doi:10.1016/j.jflm.2008.07.013. PMID 19239964.
  14. ↑ Kodali S, Jamieson WR, Leia-Stephens M, Miyagishima RT, Janusz MT, Tyers GF (November 1991). "Traumatic rupture of the thoracic aorta. A 20-year review: 1969-1989". Circulation 84 (5 Suppl): III40–6. PMID 1934437.
  15. ↑ Finkelmeier BA (September 1997). "Dissection of the aorta: a clinical update". J Vasc Nurs 15 (3): 88-93. PMID 9362838.
  16. ↑ 16.0 16.1 16.2 Blumbergs PC, Jones NR, North JB (July 1989). "Diffuse axonal injury in head trauma". J. Neurol. Neurosurg. Psychiatr. 52 (7): 838–41. PMC 1031929. PMID 2769276. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1031929/.
  17. ↑ Gleckman AM, Bell MD, Evans RJ, Smith TW (February 1999). "Diffuse axonal injury in infants with nonaccidental craniocerebral trauma: enhanced detection by beta-amyloid precursor protein immunohistochemical staining". Arch. Pathol. Lab. Med. 123 (2): 146–51. PMID 10050789.
  18. ↑ 18.0 18.1 18.2 Wetli CV, Mash D, Karch SB (July 1996). "Cocaine-associated agitated delirium and the neuroleptic malignant syndrome". Am J Emerg Med 14 (4): 425–8. PMID 8768172.
  19. ↑ http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=2374865
  20. ↑ Pollanen, MS.; Chiasson, DA.; Cairns, JT.; Young, JG. (Jun 1998). "Unexpected death related to restraint for excited delirium: a retrospective study of deaths in police custody and in the community.". CMAJ 158 (12): 1603-7. PMC 1229410. PMID 9645173. http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=1229410.
  21. ↑ Stephens BG, Jentzen JM, Karch S, Wetli CV, Mash DC (March 2004). "National Association of Medical Examiners position paper on the certification of cocaine-related deaths". Am J Forensic Med Pathol 25 (1): 11–3. PMID 15075681.
  22. ↑ Saukko, Pekka; Knight, Bernard (2004). Knight's Forensic Pathology (3rd ed.). A Hodder Arnold Publication. pp. 589. ISBN 978-0340760444.
  23. ↑ Simons FE (February 2010). "Anaphylaxis". J. Allergy Clin. Immunol. 125 (2 Suppl 2): S161–81. doi:10.1016/j.jaci.2009.12.981. PMID 20176258.
  24. ↑ http://www.ncbi.nlm.nih.gov/pubmed/16410164 http://www.ncbi.nlm.nih.gov/pubmed/17952460
  25. ↑ Sarvesvaran R, Knight BH (December 1994). "The examination of skeletal remains". Malays J Pathol 16 (2): 117–26. PMID 9053560.
  26. ↑ Moore Keith L.; Dalley Arthur F. (1999). Clinically Oriented Anatomy (4th ed.). Lippincott Williams & Wilkins. pp. 336. ISBN 978-0683061413.
  27. ↑ 27.0 27.1 URL: http://anthropology.si.edu/writteninbone/comic/activity/pdf/Skeleton_male_or_female.pdf. Accessed on: 8 September 2010.
  28. ↑ URL: http://commons.wikimedia.org/wiki/File:Gray132.png. Accessed on: 9 September 2010.
  29. ↑ Anderson JY, Trinkaus E (February 1998). "Patterns of sexual, bilateral and interpopulational variation in human femoral neck-shaft angles". J. Anat. 192 ( Pt 2): 279–85. PMC 1467761. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1467761/.

External links

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