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Difference between revisions of "Light microscopy"
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It follows from the above equation that, closure of the condenser diaphragm results in a loss of resolution, i.e. R is larger.<ref name=pom/><br> | It follows from the above equation that, closure of the condenser diaphragm results in a loss of resolution, i.e. R is larger.<ref name=pom/><br> | ||
Stated differently:<ref>URL: [http://www.microbehunter.com/2008/12/18/the-condenser-aperture-diaphragm/ http://www.microbehunter.com/2008/12/18/the-condenser-aperture-diaphragm/]. Accessed on: 21 January 2011.</ref> | Stated differently:<ref>URL: [http://www.microbehunter.com/2008/12/18/the-condenser-aperture-diaphragm/ http://www.microbehunter.com/2008/12/18/the-condenser-aperture-diaphragm/]. Accessed on: 21 January 2011.</ref><ref>URL: [http://www.grayfieldoptical.com/depth_of_fieldfocus.html http://www.grayfieldoptical.com/depth_of_fieldfocus.html]. Accessed on: 27 May 2011.</ref> | ||
*Opening the condenser --> increases resolution & brightness -- but -- decreases depth of field (DOF) & contrast. | *Opening the condenser --> increases resolution & brightness -- but -- decreases depth of field (DOF) & contrast. | ||
*Closing the condenser --> increases DOF & contrast -- but -- decreases resolution & brightness. | *Closing the condenser --> increases DOF & contrast -- but -- decreases resolution & brightness. | ||