48,830
edits
Difference between revisions of "Cytogenetics"
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→Karyotyping: more
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****Examples: Q-banding (Q=Quinacrine), C-banding (C=constitutive heterchromatin), G-banding (G=Giemsa), R-banding (R=reverse). | ****Examples: Q-banding (Q=Quinacrine), C-banding (C=constitutive heterchromatin), G-banding (G=Giemsa), R-banding (R=reverse). | ||
*****Q-banding is the standard at UHN. | *****Q-banding is the standard at UHN. | ||
Analysis: | |||
*Based on comparison to maps of the chromosomes (ideograms). | |||
**Detailed ideograms are in ''ISCN 2009''.<ref>{{Ref ISCN2009|}}</ref> | |||
***The detail of an ideogram is given in "band levels"; a 850 band ideogram has a higher resolution than a 400 band ideogram. The typical range for band level is 300-850. The band level, for a given specimen, is determined by an empirical standard and based on the number of bands an observer sees in a subset of the chromosomes.<ref name=pmid8291552>{{Cite journal | last1 = Welborn | first1 = JL. | last2 = Welborn | first2 = R. | title = Banding resolution of human chromosomes: a method of accuracy and simplicity. | journal = Am J Med Genet | volume = 47 | issue = 8 | pages = 1180-3 | month = Dec | year = 1993 | doi = 10.1002/ajmg.1320470810 | PMID = 8291552 }} | |||
</ref> | |||
Image: | Image: | ||