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Difference between revisions of "Molecular pathology"
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==Molecular== | ==Molecular== | ||
General | ===General=== | ||
*Very small changes - submicroscopic. | *Very small changes - submicroscopic. | ||
*Sequence data. | *Sequence data. | ||
Techniques | ===Techniques=== | ||
*DNA sequencing. | *DNA sequencing. | ||
**Real time-PCR, [[AKA]] real time-quantitative PCR (RQ-PCR). | **Real time-PCR, [[AKA]] real time-quantitative PCR (RQ-PCR). | ||
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****If the mutation is absent no PCR product is seen. | ****If the mutation is absent no PCR product is seen. | ||
=== | ====Specific tests==== | ||
A list of tests are found in the ''[[Molecular pathology tests]]'' article. | A list of tests are found in the ''[[Molecular pathology tests]]'' article. | ||
===DNA & RNA extraction=== | ====DNA & RNA extraction==== | ||
*Techniques are largely standardized. | *Techniques are largely standardized. | ||
*Protocols exist for fresh tissue and formulin fixed parafin imbeded tissue. | *Protocols exist for fresh tissue and formulin fixed parafin imbeded tissue. | ||
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==Cytogenetics== | ==Cytogenetics== | ||
General | ===General=== | ||
*Large changes (chromosomal). | *Large changes (chromosomal). | ||
**Maximum resolution 3-4 megabase pairs (3-4 million base pairs); may be less - dependent on band density.<ref>{{Ref WMSP|695}}</ref> | **Maximum resolution 3-4 megabase pairs (3-4 million base pairs); may be less - dependent on band density.<ref>{{Ref WMSP|695}}</ref> | ||
*Morphologic data. | *Morphologic data. | ||
=== | ===Techniques=== | ||
*ISH = in situ hybridization. | *ISH = in situ hybridization. | ||
**FISH = fluorescent in situ hybridization. | **FISH = fluorescent in situ hybridization. | ||
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*[http://commons.wikimedia.org/wiki/File:Bcrablmet.jpg Bcr-Abl translocation (WC)]. | *[http://commons.wikimedia.org/wiki/File:Bcrablmet.jpg Bcr-Abl translocation (WC)]. | ||
==World protein databank== | ==Other garbage== | ||
===World protein databank=== | |||
I can't help think it is ironic that the protein databank goal is to maintain a free and publicly available archive,<ref>Worldwide Protein Data Bank. URL: [http://www.wwpdb.org/faq.html http://www.wwpdb.org/faq.html] Accessed on: April 22, 2009.</ref> yet the announcement is in pay-for-access journal (''Nature Structual Biology'').<ref name=pmid14634627>{{cite journal |author=Berman H, Henrick K, Nakamura H |title=Announcing the worldwide Protein Data Bank |journal=Nat. Struct. Biol. |volume=10 |issue=12 |pages=980 |year=2003 |month=December |pmid=14634627 |doi=10.1038/nsb1203-980 |url=}}</ref> | I can't help think it is ironic that the protein databank goal is to maintain a free and publicly available archive,<ref>Worldwide Protein Data Bank. URL: [http://www.wwpdb.org/faq.html http://www.wwpdb.org/faq.html] Accessed on: April 22, 2009.</ref> yet the announcement is in pay-for-access journal (''Nature Structual Biology'').<ref name=pmid14634627>{{cite journal |author=Berman H, Henrick K, Nakamura H |title=Announcing the worldwide Protein Data Bank |journal=Nat. Struct. Biol. |volume=10 |issue=12 |pages=980 |year=2003 |month=December |pmid=14634627 |doi=10.1038/nsb1203-980 |url=}}</ref> | ||
==Wnt/beta-catenin pathway== | ===Wnt/beta-catenin pathway=== | ||
Important in hepatoblastomas.<ref name=Ref_PBoD923>{{Ref PBoD|923}}</ref> | Important in hepatoblastomas.<ref name=Ref_PBoD923>{{Ref PBoD|923}}</ref> | ||