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Difference between revisions of "Molecular pathology"
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→Cytogenetics: more
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*Morphologic data. | *Morphologic data. | ||
===Overview=== | |||
Techniques: | Techniques: | ||
*ISH = in situ hybridization. | *ISH = in situ hybridization. | ||
**FISH = fluorescent in situ hybridization. | **FISH = fluorescent in situ hybridization. | ||
**SISH = silver in situ hybridization.<ref>URL: [http://www.immunoportal.com/modules.php?name=News&file=article&sid=186 http://www.immunoportal.com/modules.php?name=News&file=article&sid=186]. Accessed on: 2 May 2011.</ref> | **SISH = silver in situ hybridization.<ref>URL: [http://www.immunoportal.com/modules.php?name=News&file=article&sid=186 http://www.immunoportal.com/modules.php?name=News&file=article&sid=186]. Accessed on: 2 May 2011.</ref> | ||
*Karyotyping. | |||
====Karyotyping==== | |||
*Metaphase nuclei. | |||
====ISH==== | |||
*Usu. interphase nuclei. | |||
May be prepared from: | |||
*In situ paraffin sections (section 4 micrometers thick). | |||
*Isolated nuclei (section 10 micrometers thick). | |||
*Cytospin. | |||
*Cultured cells. | |||
=====Probe types===== | |||
Types: | |||
*Fusion (two colours). | |||
*Breakapart (two colours). | |||
*Deletion/duplication (e.g. trisomy). | |||
Image: | Image: | ||