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Difference between revisions of "Immunohistochemical staining"
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Immunohistochemical staining (view source)
Revision as of 11:48, 30 November 2020
, 11:48, 30 November 2020→Signal assessment: Update
Jensflorian (talk | contribs) (→Theory: Update) |
Jensflorian (talk | contribs) (→Signal assessment: Update) |
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===Signal assessment=== | ===Signal assessment=== | ||
*Manual | *Manual vs. automated counting. | ||
*Choice of chromogen | *Choice of proper chromogen. | ||
**Dynamic range of DAB is 1-2logs vs. fluorescent probes 2-3logs. | **Dynamic range of DAB is 1-2logs vs. fluorescent probes 2-3logs. | ||
**Protein expression range can be up to 4 logs in gene amplification (information is missed in IHC). | **Protein expression range can be up to 4 logs in gene amplification (information is missed in IHC). | ||
**FastRed stains are more suited in melanocytic tumors than DAB. | **FastRed stains are more suited in melanocytic tumors than DAB. | ||
* | *Use of established cutoffs for "intensity" and for "positive" staining. | ||
*Visual pattern recogniton vs. detecting spatial subtle changes. | |||
*Detecting differences at low intensity with human eye is less accurate. | |||
==Quality control== | ==Quality control== | ||