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Difference between revisions of "Immunohistochemical staining"
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Immunohistochemical staining (view source)
Revision as of 14:38, 24 September 2015
, 14:38, 24 September 2015→Interpretation: base error rate
Jensflorian (talk | contribs) (wikify INI1 p63 STAT6) |
Jensflorian (talk | contribs) (→Interpretation: base error rate) |
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#*A combination of the above. | #*A combination of the above. | ||
Generally, interpretations can be subjective, and this is especially true when the staining is weak and focal. In other words, "... your weak [positive] stain might be somebody else’s negative."<ref>URL: [http://bitesizebio.com/articles/immunohistochemistry-getting-the-stain-you-want/ http://bitesizebio.com/articles/immunohistochemistry-getting-the-stain-you-want/]. Accessed on: 1 September 2012.</ref> | Generally, interpretations can be subjective, and this is especially true when the staining is weak and focal. In other words, "... your weak [positive] stain might be somebody else’s negative."<ref>URL: [http://bitesizebio.com/articles/immunohistochemistry-getting-the-stain-you-want/ http://bitesizebio.com/articles/immunohistochemistry-getting-the-stain-you-want/]. Accessed on: 1 September 2012.</ref> This is often reflected in publications repoting contradictory results regarding the rates of positivity for stains in different tumours, even if the medthods uses are identical. | ||
The cynical might say it is unwritten rule that: "... if the stain is weak and focal it can be anything you want to make it -- positive or negative -- so it fits perfectly with your diagnosis!" | The cynical might say it is unwritten rule that: "... if the stain is weak and focal it can be anything you want to make it -- positive or negative -- so it fits perfectly with your diagnosis!" | ||
In cases where the morphology is unclear, it is judicious to have two or more immunostains that support the diagnosis, and negative stains for important entities in the differential diagnosis. | In cases where the morphology is unclear, it is judicious to have two or more immunostains that support the diagnosis, and negative stains for important entities in the differential diagnosis. | ||
One third pathologists substantially overestimate and one third underestimate the diagnostic significance of unexpected immunohistochemical staining results.<ref>{{Cite journal | last1 = Galloway | first1 = M. | title = Base-rate error in the interpretation of immunohistochemistry. | journal = Patholog Res Int | volume = 2011 | issue = | pages = 636495 | month = | year = 2011 | doi = 10.4061/2011/636495 | PMID = 21660231 }}</ref> | |||
==General (malignant) differential diagnosis== | ==General (malignant) differential diagnosis== | ||