Difference between revisions of "Libre Pathology talk:Study Group"

{{hidden|List three differences between DNA and RNA.|<left>[[DNA (double stranded, Thymine, deoxyribose, more stable; RNA single stranded, ribose, uracil]]</left>}}

{{hidden|What are the three stop codons?|<center>[[UAA, UGA, UAG]]</center>}}

{{hidden|Where does transcription begin?|<center>[[promoters at the 5' end  before the coding region]]</center>}}

{{hidden|List 2 enzymes necessary for transcription and their function. |<center>[[helicase, polymerase]]</center>}}

{{hidden|List and describe three post transcription modifications of RNA.|<center>[[Splicing, cappping, 3'polyadenylation, ]]</center>}}

 

{{hidden|List three differences between somatic and germline mutations. |<center>[[Microsatellite instability]]</center>}}

{{hidden|What is the difference between a missense and a non-sense mutation?|<center>[[Microsatellite instability]]</center>}}

{{hidden|Define a frameshift mutation. |<center>[[Microsatellite instability]]</center>}}

{{hidden|Why are inversion mutations difficult to detect?|<center>[[Microsatellite instability]]</center>}}

{{hidden|Describe the potential sequelae of a translocation mutation. |<center>[[Microsatellite instability]]</center>}}

{{hidden|Translate the following: c.1524_1527delCGTA.|<center>[[Microsatellite instability]]</center>}}

{{hidden|List 5 features of SNPs.|<center>[[Microsatellite instability]]</center>}}

{{hidden|Define a regulatory SNP and a synonymous SNP?|<center>[[Microsatellite instability]]</center>}}

{{hidden|What is the difference between a microstalellite and a minisattelite?|<center>[[Microsatellite instability]]</center>}}

{{hidden|Describe Hardy-Weinberg Equilibrium?|<center>[[Microsatellite instability]]</center>}}

{{hidden|What factors can disrupt the H-W equilibrium?|<center>[[Microsatellite instability]]</center>}}

{{hidden|What is linkage disequilibrium?|<center>[[Microsatellite instability]]</center>}}

{{hidden|What are the three major steps of PCR?|<center>[[Microsatellite instability]]</center>}}

{{hidden|What is the hallmark of PCR?|<center>[[Microsatellite instability]]</center>}}

{{hidden|What factors affect the method of genotyping chosen?|<center>[[Microsatellite instability]]</center>}}

{{hidden|Define sensitivity, specificity, positive predictive value and negative predictive value. |<center>[[Microsatellite instability]]</center>}}

{{hidden|Define reproducibility and accuracy of an analytical test. |<center>[[Microsatellite instability]]</center>}}

{{hidden|Describe briefly Sanger sequencing.|<center>[[Microsatellite instability]]</center>}}

{{hidden|Describe briefly how Taqman automated genotyping is used for allele detection. |<center>[[Microsatellite instability]]</center>}}

{{hidden|How are DNA microarrays used to identify drug disposition or responses?|<center>[[Microsatellite instability]]</center>}}

{{hidden|Describe the procedure for submitting FFPE slides for KRAS for colorectal cancer.|<center>[[Microsatellite instability]]</center>}}

{{hidden|Compare and contrast uniplex versus multiplex genotyping. |<center>[[Microsatellite instability]]</center>}}

{{hidden|Compare and contrast conventional vs massively parallel sequencing. |<center>[[Microsatellite instability]]</center>}}

{{hidden|What is multiplex ligation-dependent ligation (MLPA)?|<center>[[Microsatellite instability]]</center>}}

{{hidden|What is fragment analysis?|<center>[[Microsatellite instability]]</center>}}

{{hidden|Compare and contrast RT-PCR vs qRTPCR.|<center>[[Microsatellite instability]]</center>}}

{{hidden|What is MSI?|<center>[[Microsatellite instability]]</center>}}

{{hidden|What is methylation analysis?|<center>[[Microsatellite instability]]</center>}}

{{hidden|What are the four test features required to be documented by the CLIA?|<center>[[Microsatellite instability]]</center>}}

{{hidden|What are "in vitro diagnostics" vs "laboratory developed tests"?|<center>[[Microsatellite instability]]</center>}}

{{hidden|What does validation mean? |<center>[[Microsatellite instability]]</center>}}

{{hidden|What are the four performance characteristics that need to be verified for FDA cleared/approved tests?|<center>[[Microsatellite instability]]</center>}}

{{hidden|What are the six performance characteristics that need to be verified for FDA cleared LDTs or modified FDA cleared/approved tests?|<center>[[Microsatellite instability]]</center>}}

{{hidden|List the components of a molecular pathology report.|<center>[[Microsatellite instability]]</center>}}

{{hidden|Define analytical sensitivity and clinical sensitivity. |<center>[[Microsatellite instability]]</center>}}

{{hidden|What should be said in a report of a molecular test on a patient for residual disease if no previous positive assay was confirmed?|<center>[[Microsatellite instability]]</center>}}

{{hidden|Define ammended report versus addendum report.|<center>[[Microsatellite instability]]</center>}}

{{hidden|Whose responsibility is it to sythesize the test results with other clinico-pathological information?|<center>[[Microsatellite instability]]</center>}}

{{hidden|How long are cytogenetic reports required to be kept by CAP?|<center>[[Microsatellite instability]]</center>}}

{{hidden|What is the recommended process to use test results if an assay is not yet validated for clinical use?|<center>[[Microsatellite instability]]</center>}}

{{hidden|Give three examples of "grey areas" which warrant discretion of professionals involved to use a non-validated test?|<center>[[Microsatellite instability]]</center>}}

{{hidden|What reference standard is available for gene nomenclature?|<center>[[Microsatellite instability]]</center>}}

{{hidden|Create a table of the most common gene rearrangements associated with heme and soft tissue diseases. |<center>[[Microsatellite instability]]</center>}}

{{hidden|What is a "DNA fingerprint" and what can it be used for?|<center>[[A method that examines multiple areas of short tandem repeats to identify paternity, mosaicism, chimerism, and identity in forensics cases]]</center>}}

2. Constitutional vs somatic mutations.

 

 

Hi Michael, I've started, but mostly just with the questions for now, as I study I will keep working on it. Can you help me, maybe we can make additional discussion pages for each of my "study" exams,e.g. molecular, robbins chapters, cap protocols etc. This is just like LaTEX!!!