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Difference between revisions of "Immunohistochemical staining"
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Immunohistochemical staining (view source)
Revision as of 03:18, 12 December 2010
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Image: [http://commons.wikimedia.org/wiki/File:Lung_adenocarcinoma_-_TTF-1_-_high_mag.jpg Adenocarcinoma with nuclear TTF-1 positivity (WC)]. | Image: [http://commons.wikimedia.org/wiki/File:Lung_adenocarcinoma_-_TTF-1_-_high_mag.jpg Adenocarcinoma with nuclear TTF-1 positivity (WC)]. | ||
=== | ===Breast markers=== | ||
*GCDFP-15 ( | *GCDFP-15 ([[AKA]] BRST-2) -- specific, but NOT sensitive. | ||
*ER (estrogen receptor) - in normal breast. | *ER (estrogen receptor) - in normal [[breast]]. | ||
*PR (progesterone receptor) - in normal breast. | *PR (progesterone receptor) - in normal breast. | ||
*HER2/neu - pathological, assoc. with worse prognosis. | *HER2/neu - pathological, assoc. with worse prognosis. | ||
**HER2/neu+ cancers Tx'ed with trastuzumab (Herceptin). | **HER2/neu+ cancers Tx'ed with trastuzumab (Herceptin). | ||
===Prostate=== | ===Prostate gland=== | ||
*PSA - prostatic specific antigen. | *PSA - [[prostate gland|prostatic]] specific antigen. | ||
*PSAP - prostatic specific acid phosphatase. | *PSAP - prostatic specific acid phosphatase. | ||
*p63 - stains nuclei of basal cell in normal [[prostate]]. | *p63 - stains nuclei of basal cell in normal [[prostate]]. | ||
*34betaE12 - stains basals cells in normal prostate. | *34betaE12 - stains basals cells in normal prostate. | ||
*AMACR (racemase, P504S<ref>[http://www.antibodies-online.com/antibody/125649/P504S+alphaMethylacylCoA+Racemace+AMACR+Human/ http://www.antibodies-online.com/antibody/125649/P504S+alphaMethylacylCoA+Racemace+AMACR+Human/]</ref>) - present in adenocarcinoma (NOT in normal prostate). | *AMACR (racemase, P504S<ref>[http://www.antibodies-online.com/antibody/125649/P504S+alphaMethylacylCoA+Racemace+AMACR+Human/ http://www.antibodies-online.com/antibody/125649/P504S+alphaMethylacylCoA+Racemace+AMACR+Human/]</ref>) - present in adenocarcinoma (NOT in normal prostate). | ||
*CAP cocktail - AKA ''CAP'' (AMACR | *CAP cocktail - AKA ''CAP'' (AMACR, p63 and 34βE12). | ||
**Image: [http://www.webpathology.com/image.asp?case=96&n=5 CAP CAP cocktail (webpathology.com)]. | **Image: [http://www.webpathology.com/image.asp?case=96&n=5 CAP CAP cocktail (webpathology.com)]. | ||
=== | ===[[Colorectal tumours|Colorectal carcinoma]] markers=== | ||
*CEA. | *CEA. | ||
===Small bowel=== | ===[[Small bowel]]=== | ||
*CDX2. | *CDX2. | ||
*Villin. | *Villin. | ||
===Kidney=== | ===[[Kidney tumours|Kidney]]=== | ||
[[Renal cell carcinoma]]: | [[Renal cell carcinoma]]: | ||
*RCC, EMA, CD10. | *RCC, EMA, CD10. | ||
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*CD68 (for macrophages). | *CD68 (for macrophages). | ||
===Ovary=== | ===[[Ovarian tumours|Ovary]]=== | ||
*CA125, CK7+, CK20-. | *CA125, CK7+, CK20-. | ||
*WT1 -- 90% in serous +ve. | *WT1 -- 90% in serous +ve. | ||
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*WT-1, CA-125, D2-40. | *WT-1, CA-125, D2-40. | ||
===Liver=== | ===[[Liver neoplasms|Liver]]=== | ||
*AFP (alpha-fetaprotein). | *AFP (alpha-fetaprotein). | ||
*Glypican-3. | *Glypican-3. | ||
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*CK19.<ref name=pmid18318589>{{cite journal |author=Geller SA, Dhall D, Alsabeh R |title=Application of immunohistochemistry to liver and gastrointestinal neoplasms: liver, stomach, colon, and pancreas |journal=Arch. Pathol. Lab. Med. |volume=132 |issue=3 |pages=490–9 |year=2008 |month=March |pmid=18318589 |doi= |url=}}</ref> | *CK19.<ref name=pmid18318589>{{cite journal |author=Geller SA, Dhall D, Alsabeh R |title=Application of immunohistochemistry to liver and gastrointestinal neoplasms: liver, stomach, colon, and pancreas |journal=Arch. Pathol. Lab. Med. |volume=132 |issue=3 |pages=490–9 |year=2008 |month=March |pmid=18318589 |doi= |url=}}</ref> | ||
===Neuropathology=== | ===[[Neuropathology]]=== | ||
General: | General: | ||
*S-100. | *S-100. | ||
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Specific entities: | Specific entities: | ||
*EMA +ve: meningioma, ependymomas (cytoplasm dot-like).<ref name=Ref_PSNP12>{{Ref PSNP|12}}</ref> | *EMA +ve: meningioma, ependymomas (cytoplasm dot-like).<ref name=Ref_PSNP12>{{Ref PSNP|12}}</ref> | ||
Tumour (low-grade gliomas): | |||
*IDH-1 +ve. | |||
**Usually negative in Glioblastoma. | |||
==Miscellaneous== | ==Miscellaneous== | ||
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===Lymphocytes=== | ===Lymphocytes=== | ||
*CD45 (AKA ''common leukocyte antigen''). | *CD45 ([[AKA]] ''common leukocyte antigen''). | ||
===B-cells=== | ===B-cells=== | ||
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*CD15 Reed-Sternberg cells +ve ~80%, stains neutrophils. | *CD15 Reed-Sternberg cells +ve ~80%, stains neutrophils. | ||
==Germ cell | ==[[Germ cell tumour]]s== | ||
===Seminoma=== | ===Seminoma=== | ||
*D2-40 +ve.<ref name=pmid18045648/> | *D2-40 +ve.<ref name=pmid18045648/> | ||