Difference between revisions of "Haematopathology"

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Haematopathology (view source)

Revision as of 20:53, 11 August 2010

885 bytes removed ,  20:53, 11 August 2010
→‎Population cell marker quantification: shorten mv to cytometry
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==Population cell marker quantification==
==Cytometry - population cell marker quantification==
Two techniques:
{{main|cytometry}}
*[[Flow cytometry]].
*Laser scanning cytometry (LSC).


Common markers:
===Two techniques===
#[[Flow cytometry]].
#Laser scanning cytometry (LSC).
 
===Common markers===
*CD3, CD4, CD8, CD5, CD7.
*CD3, CD4, CD8, CD5, CD7.
*CD19, CD20, FMC7.
*CD19, CD20, FMC7.
*Kappa, lambda.
*Kappa, lambda.


Normal:
===Normal===
*T-cells to B-cells usually 1:1.
*T-cells to B-cells usually 1:1.
*In reactive nodes T-cell predominate.
*In reactive nodes T-cells predominate.
*Normal thymic tissue has cells that are positive for both CD4 and CD8.
*Normal thymic tissue has cells that are positive for both CD4 and CD8.
*Kappa (k) and lambda (l) are not expressed by the same cell.
*Kappa and lambda are not expressed by the same cell.
*Rule-of-thumb for normal k:l range is: <6:1 and 1:<3.<ref>SB. March 10, 2010.</ref>  
*Rule-of-thumb for normal k:l range is: <6:1 and 1:<3.<ref>SB. March 10, 2010.</ref>  
**Lambda dominance is less common.
**Lambda dominance is less common.
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*CD7 = the smallest number of T-cell
*CD7 = the smallest number of T-cell


Abnormal:
===Abnormal===
*CD4>CD8 predominance classically seen in:
See ''[[cytometry]]''.
**Sarcoidosis.
**Hodgkin's lymphoma.
*T cell lymphoma - loss of one of the T-cell markers (CD2, CD3, CD5 or CD7).
**CD7 is usually the first to be lost.
 
===Sample report===
B-Cell Associated Markers
*CD10.
**Germinal centre marker.
**+ve in: follicular l., Burkitt l., precursor lymphoblastic leukemia.
*CD19.
**B cell marker.
**Should be ~=CD20.
*CD20.
**B cell marker.
**Should be ~=CD20.
*CD23.
**+ve in CLL/SLL.
*FMC7
**B cell marker.
*Kappa+CD19/20+.
**B cell clonality.
*Lambda+CD19/20+
**B cell clonality.
T-Cell Associated Markers
*CD3.
**CD3 ~= CD4 + CD8.
**Usually: CD2 > CD3 > CD7.
*CD5.
**Usually: CD2 > CD5 > CD7.
*CD4.
**CD3 ~= CD4 + CD8.
*CD8.
**CD3 ~= CD4 + CD8.
*CD7.
**Usually least prevalent T cell marker; < CD2, < CD3, < CD5.
*CD2.
**Usually most prevalent T cell marker; > CD3, > CD5, > CD7.
NK-Cell Associated Markers
*CD56.
*CD16.
Miscellaneous Markers
*CD11c.
*CD14.


==See also==
==See also==
Michael
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