From Libre Pathology
Flow cytometry is an automated technique used to examine cells that is widely used for the diagnosis and characterization of lymphoma.
- Label cells with fluorescent antibodies.
- Passed through a channel one-at-a-time (using hydrodynamic focusing) and interrogated with a laser light.
Scatter of light
- Forward scatter (FS) -- indicative of cell size.
- Side scatter (SS) -- indicative of cell complexity (granularity).
Scatter in a table
Selected white blood cells & scatter:
|Forward scatter||Side scatter|
- Tissue must be fresh, i.e. cannot be formalin fixed.
- Viable material may be salvaged from the centre of a large specimen placed in formalin for a short time.
- 2 mm x 2 mm x 2 mm piece of tissue is sufficient.
- 1 mm3 is considered the minimum by Mayo Medical Laboratories.
- See Cytometry.
- URL: http://www.med.umich.edu/flowcytometry/InitialTraining/lessons/lesson1/index.htm. Accessed on: 5 August 2010.
- URL: http://www.med.umich.edu/flowcytometry/InitialTraining/lessons/lesson1/images/blood.gif. Accessed on: 5 August 2010.
- URL: http://www.mayomedicallaboratories.com/test-catalog/Specimen/19499. Accessed on: 27 December 2012.