Difference between revisions of "Amebiasis"

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{{ Infobox diagnosis
| Name      = {{PAGENAME}}
| Image      = Amebiasis - very high mag.jpg
| Width      =
| Caption    = Amebiasis. [[H&E stain]].
| Synonyms  =
| Micro      = entamoeba histolytica: round/ovoid eosinophilic bodies ~ 40-60 micrometers in maximal dimension; found in bowel lumen, usu. ingest (whole) [[red blood cell]]s
| Subtypes  =
| LMDDx      = [[fecal material]], necroinflammatory debris
| Stains    =
| IHC        =
| EM        =
| Molecular  = PCR testing
| IF        =
| Gross      =
| Grossing  =
| Site      = [[colon]]
| Assdx      = +/-[[liver abscess]]
| Syndromes  =
| Clinicalhx =
| Signs      = diarrhea, blood per rectum
| Symptoms  =
| Prevalence = uncommon
| Bloodwork  =
| Rads      =
| Endoscopy  = [[colitis]], mass lesion, [[granulation tissue]]-like
| Prognosis  = benign
| Other      =
| ClinDDx    = [[colorectal carcinoma]], [[colitis]], [[granulation tissue]]
| Tx        =
}}
'''Amebiasis''', also be spelled '''amoebiasis''', is an infectious disease, caused by the protozoan ''Entamoeba histolytica''.
'''Amebiasis''', also be spelled '''amoebiasis''', is an infectious disease, caused by the protozoan ''Entamoeba histolytica''.


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*[[Colitis]].
*[[Colitis]].
*[[Liver abscess]].
*[[Liver abscess]].
==Gross==
Features:<ref name=pmid8255341>{{Cite journal  | last1 = Stuiver | first1 = PC. | last2 = Visser | first2 = LG. | title = [Ameboma of the large intestine and rectum]. | journal = Ned Tijdschr Geneeskd | volume = 137 | issue = 45 | pages = 2328-31 | month = Nov | year = 1993 | doi =  | PMID = 8255341 }}</ref>
*+/-Mass.
**May mimic carcinoma.<ref name=pmid12750955>{{Cite journal  | last1 = Ooi | first1 = BS. | last2 = Seow-Choen | first2 = F. | title = Endoscopic view of rectal amebiasis mimicking a carcinoma. | journal = Tech Coloproctol | volume = 7 | issue = 1 | pages = 51-3 | month = Apr | year = 2003 | doi = 10.1007/s101510300008 | PMID = 12750955 }}</ref>
*+/-[[Granulation tissue]]-like appearance.


==Microscopic==
==Microscopic==
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Image:Amoebic_dysentery_in_colon_biopsy_%281%29.jpg | Amebiasis (WC)
Image:Amoebic_dysentery_in_colon_biopsy_%281%29.jpg | Amebiasis (WC)
</gallery>
</gallery>
==Stains==
*Iron-hematoxylin stain - black.<ref name=pimd22523839>{{Cite journal  | last1 = Chacín-Bonilla | first1 = L. | title = [Microscopic diagnosis of amebiasis: an obsolete method but necessary in the developing world]. | journal = Invest Clin | volume = 52 | issue = 4 | pages = 291-4 | month = Dec | year = 2011 | doi =  | PMID = 22523839 }}</ref><ref>URL: [http://www.histopathology-india.net/amco.htm http://www.histopathology-india.net/amco.htm]. Accessed on: 29 June 2016.</ref>
*Trichrome stain.<ref name=pmid2454958>{{Cite journal  | last1 = Shetty | first1 = N. | last2 = Prabhu | first2 = T. | title = Evaluation of faecal preservation and staining methods in the diagnosis of acute amoebiasis and giardiasis. | journal = J Clin Pathol | volume = 41 | issue = 6 | pages = 694-9 | month = Jun | year = 1988 | doi =  | PMID = 2454958 }}</ref><ref>URL: [http://emedicine.medscape.com/article/212029-workup http://emedicine.medscape.com/article/212029-workup]. Accessed on: 29 June 2016.</ref>
==IHC==
*Entamoeba histolytica +ve.<ref name=pmid1283004>{{Cite journal  | last1 = Prasad | first1 = R. | last2 = Tola | first2 = M. | last3 = Bhattacharya | first3 = S. | last4 = Sharma | first4 = MP. | last5 = Bhattacharya | first5 = A. | title = Recognition of Entamoeba histolytica lipophosphoglycan by a strain-specific monoclonal antibody and human immune sera. | journal = Mol Biochem Parasitol | volume = 56 | issue = 2 | pages = 279-87 | month = Dec | year = 1992 | doi =  | PMID = 1283004 }}</ref>
==Molecular==
*May be identified by [[PCR]] with an appropriate primer.<ref name=pmid24192618>{{Cite journal  | last1 = Zeyrek | first1 = FY. | last2 = Turgay | first2 = N. | last3 = Unver | first3 = A. | last4 = Ustün | first4 = S. | last5 = Akarca | first5 = U. | last6 = Töz | first6 = S. | title = Differentiation of Entamoeba histolytica/Entamoeba dispar by the polymerase chain reaction in stool samples of patients with gastrointestinal symptoms in the Sanliurfa Province. | journal = Turkiye Parazitol Derg | volume = 37 | issue = 3 | pages = 174-8 | month =  | year = 2013 | doi = 10.5152/tpd.2013.39 | PMID = 24192618 }}</ref>


==See also==
==See also==
*[[Colon]].
*[[Colon]].
*[[Red blood cells]].


==References==
==References==

Latest revision as of 12:50, 29 June 2016

Amebiasis
Diagnosis in short

Amebiasis. H&E stain.
LM entamoeba histolytica: round/ovoid eosinophilic bodies ~ 40-60 micrometers in maximal dimension; found in bowel lumen, usu. ingest (whole) red blood cells
LM DDx fecal material, necroinflammatory debris
Molecular PCR testing
Site colon
Associated Dx +/-liver abscess
Signs diarrhea, blood per rectum
Prevalence uncommon
Endoscopy colitis, mass lesion, granulation tissue-like
Prognosis benign
Clin. DDx colorectal carcinoma, colitis, granulation tissue

Amebiasis, also be spelled amoebiasis, is an infectious disease, caused by the protozoan Entamoeba histolytica.

General

May cause:[3]

Gross

Features:[4]

Microscopic

Features:

  • Entamoeba histolytica are round/ovoid eosinophilic bodies ~ 40-60 micrometers in maximal dimension.
    • Found in bowel lumen.
    • Ingest RBCs.

Image

  • Amebiasis - high mag. (WC/Nephron)

  • Amebiasis - very high mag. (WC/Nephron)

  • Amebiasis (WC)

Stains

  • Iron-hematoxylin stain - black.[6][7]
  • Trichrome stain.[8][9]

IHC

  • Entamoeba histolytica +ve.[10]

Molecular

  • May be identified by PCR with an appropriate primer.[11]

See also

References

  1. ↑ URL: http://www.health.state.ny.us/diseases/communicable/amebiasis/fact_sheet.htm. Accessed on: 17 June 2010.
  2. ↑ Fernandes, H.; D'Souza, CR.; Swethadri, GK.; Naik, CN.. "Ameboma of the colon with amebic liver abscess mimicking metastatic colon cancer.". Indian J Pathol Microbiol 52 (2): 228-30. PMID 19332922. http://www.ijpmonline.org/article.asp?issn=0377-4929;year=2009;volume=52;issue=2;spage=228;epage=230;aulast=Fernandes.
  3. ↑ Mortimer, L.; Chadee, K. (Mar 2010). "The immunopathogenesis of Entamoeba histolytica.". Exp Parasitol. doi:10.1016/j.exppara.2010.03.005. PMID 20303955.
  4. ↑ Stuiver, PC.; Visser, LG. (Nov 1993). "[Ameboma of the large intestine and rectum].". Ned Tijdschr Geneeskd 137 (45): 2328-31. PMID 8255341.
  5. ↑ Ooi, BS.; Seow-Choen, F. (Apr 2003). "Endoscopic view of rectal amebiasis mimicking a carcinoma.". Tech Coloproctol 7 (1): 51-3. doi:10.1007/s101510300008. PMID 12750955.
  6. ↑ Chacín-Bonilla, L. (Dec 2011). "[Microscopic diagnosis of amebiasis: an obsolete method but necessary in the developing world].". Invest Clin 52 (4): 291-4. PMID 22523839.
  7. ↑ URL: http://www.histopathology-india.net/amco.htm. Accessed on: 29 June 2016.
  8. ↑ Shetty, N.; Prabhu, T. (Jun 1988). "Evaluation of faecal preservation and staining methods in the diagnosis of acute amoebiasis and giardiasis.". J Clin Pathol 41 (6): 694-9. PMID 2454958.
  9. ↑ URL: http://emedicine.medscape.com/article/212029-workup. Accessed on: 29 June 2016.
  10. ↑ Prasad, R.; Tola, M.; Bhattacharya, S.; Sharma, MP.; Bhattacharya, A. (Dec 1992). "Recognition of Entamoeba histolytica lipophosphoglycan by a strain-specific monoclonal antibody and human immune sera.". Mol Biochem Parasitol 56 (2): 279-87. PMID 1283004.
  11. ↑ Zeyrek, FY.; Turgay, N.; Unver, A.; Ustün, S.; Akarca, U.; Töz, S. (2013). "Differentiation of Entamoeba histolytica/Entamoeba dispar by the polymerase chain reaction in stool samples of patients with gastrointestinal symptoms in the Sanliurfa Province.". Turkiye Parazitol Derg 37 (3): 174-8. doi:10.5152/tpd.2013.39. PMID 24192618.
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