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Difference between revisions of "Stains"
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→Masson's trichrome stain: +mallory
m (→Thioflavin T) |
(→Masson's trichrome stain: +mallory) |
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How to remember? A.: Primary colours (red, blue, yellow) + black. | How to remember? A.: Primary colours (red, blue, yellow) + black. | ||
==Masson's trichrome stain== | ==Masson's trichrome stain== | ||
===General | ===General=== | ||
*Collagen vs. muscle. | *Collagen vs. muscle. | ||
===Interpretation=== | ===Interpretation=== | ||
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*Baby blue = collagen. | *Baby blue = collagen. | ||
Notes: <Ref>[http://library.med.utah.edu/WebPath/HISTHTML/MANUALS/MASSONS.PDF http://library.med.utah.edu/WebPath/HISTHTML/MANUALS/MASSONS.PDF]</ref> | Notes: <Ref>ULR: [http://library.med.utah.edu/WebPath/HISTHTML/MANUALS/MASSONS.PDF http://library.med.utah.edu/WebPath/HISTHTML/MANUALS/MASSONS.PDF]. Accessed on: 2 November 2011.</ref> | ||
===Elastic trichrome stain=== | ===Elastic trichrome stain=== | ||
| Line 372: | Line 368: | ||
Interpretation - as above in Masson's trichrome - plus: | Interpretation - as above in Masson's trichrome - plus: | ||
*Black = nuclei and '''elastin'''. | *Black = nuclei and '''elastin'''. | ||
==Mallory trichome stain== | |||
===General=== | |||
*Collagen vs. muscle. | |||
===Site=== | |||
*Kidney Bx (to assess for fibrosis). | |||
**Considered better than the [[Masson trichrome stain]]. | |||
*Liver Bx (to assess for [[cirrhosis]]). | |||
===Interpretation=== | |||
*Black = nuclei. | |||
*Red = muscle (smooth muscle actin). | |||
*Green = collagen. | |||
Image: | |||
*[http://commons.wikimedia.org/wiki/File:Cirrhosis_high_mag.jpg Mallory trichrome (WC)]. | |||
==HOPS stain== | ==HOPS stain== | ||