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Difference between revisions of "Cytogenetics"
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===Probe types=== | ===Probe types=== | ||
Types: | Types: | ||
*Fusion - usually two | *Fusion - usually two colours , e.g. [[translocations|IGH/BCL2 translocation]], BCR/ABL translocation. | ||
**Two colour - two different probes with different colours. | |||
**Two colour fusion with extra signal - similar two colour fusion.<ref name=pmid12764379>{{Cite journal | last1 = Primo | first1 = D. | last2 = Tabernero | first2 = MD. | last3 = Rasillo | first3 = A. | last4 = Sayagués | first4 = JM. | last5 = Espinosa | first5 = AB. | last6 = Chillón | first6 = MC. | last7 = Garcia-Sanz | first7 = R. | last8 = Gutierrez | first8 = N. | last9 = Giralt | first9 = M. | title = Patterns of BCR/ABL gene rearrangements by interphase fluorescence in situ hybridization (FISH) in BCR/ABL+ leukemias: incidence and underlying genetic abnormalities. | journal = Leukemia | volume = 17 | issue = 6 | pages = 1124-9 | month = Jun | year = 2003 | doi = 10.1038/sj.leu.2402963 | PMID = 12764379 | url = http://www.nature.com/leu/journal/v17/n6/full/2402963a.html }}</ref> | **Two colour fusion with extra signal - similar two colour fusion.<ref name=pmid12764379>{{Cite journal | last1 = Primo | first1 = D. | last2 = Tabernero | first2 = MD. | last3 = Rasillo | first3 = A. | last4 = Sayagués | first4 = JM. | last5 = Espinosa | first5 = AB. | last6 = Chillón | first6 = MC. | last7 = Garcia-Sanz | first7 = R. | last8 = Gutierrez | first8 = N. | last9 = Giralt | first9 = M. | title = Patterns of BCR/ABL gene rearrangements by interphase fluorescence in situ hybridization (FISH) in BCR/ABL+ leukemias: incidence and underlying genetic abnormalities. | journal = Leukemia | volume = 17 | issue = 6 | pages = 1124-9 | month = Jun | year = 2003 | doi = 10.1038/sj.leu.2402963 | PMID = 12764379 | url = http://www.nature.com/leu/journal/v17/n6/full/2402963a.html }}</ref> | ||
**Three-colour | **Three-colour - two probes (e.g. red and blue) straddle the break point.<ref>{{Cite journal | last1 = Sinclair | first1 = PB. | last2 = Green | first2 = AR. | last3 = Grace | first3 = C. | last4 = Nacheva | first4 = EP. | title = Improved sensitivity of BCR-ABL detection: a triple-probe three-color fluorescence in situ hybridization system. | journal = Blood | volume = 90 | issue = 4 | pages = 1395-402 | month = Aug | year = 1997 | doi = | PMID = 9269756 | url= http://bloodjournal.hematologylibrary.org/content/90/4/1395.full.html }}</ref> | ||
***One of the (true) translocations | ***One of the (true) translocations (e.g. presence of a Philadelphia chromosome) will have only two colours (red and green or yellow). | ||
***The derivative chromosome (e.g. derivative 9 chromosome in Philadelphia chromosome cells), which may be lost, will have all three colours. | ***The derivative chromosome (e.g. derivative 9 chromosome in Philadelphia chromosome cells), which may be lost, will have all three colours. | ||
***A (non-pathologic) coincidental colocalization (or overlap) of probes will consist of all three colours. | ***A (non-pathologic) coincidental colocalization (or overlap) of probes will consist of all three colours. | ||