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Difference between revisions of "Light microscopy"
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→Depth of field: more
(→Depth of field: more) |
(→Depth of field: more) |
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*M = magnification. | *M = magnification. | ||
*e = resolution. | *e = resolution. | ||
===Increasing the DOF=== | |||
*DOF can be increased by ''focus stacking''. | |||
Software: | |||
*[http://hugin.sourceforge.net/ Hugin (sourceforge.net)] - does ''focus stacking'' and ''stitching''. | |||
Image: | |||
*[http://commons.wikimedia.org/wiki/File:Focus_stacking_Tachinid_fly.jpg Example of focus stacking (WC)]. | |||
==Kohler illumination== | ==Kohler illumination== | ||