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Difference between revisions of "Cytogenetics"
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→In situ hybridization
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==In situ hybridization== | ==In situ hybridization== | ||
*Typically abbreviated ''ISH''. | *Typically abbreviated ''ISH''. | ||
*Usually done | |||
===Overview=== | |||
*Usually done on interphase nuclei. | |||
Comes in different flavours: | |||
*FISH = fluorescent in situ hybridization. | |||
*SISH = silver in situ hybridization.<ref>URL: [http://www.immunoportal.com/modules.php?name=News&file=article&sid=186 http://www.immunoportal.com/modules.php?name=News&file=article&sid=186]. Accessed on: 2 May 2011.</ref> | |||
May be prepared from: | May be prepared from: | ||
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===Probe types=== | ===Probe types=== | ||
Types: | Types: | ||
*Fusion (two colours). | *Fusion - two colour (two different probes with different colours). | ||
*Breakapart | *Breakapart - two colour. | ||
*Deletion/duplication (e.g. trisomy). | *Deletion/duplication (e.g. trisomy) - one colour. | ||
Image: | Image: | ||