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Difference between revisions of "Molecular pathology"
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→Cytogenetics
(→Overview: more) |
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! Other | ! Other | ||
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| ISH break apart probe (two colours) | | Interphase ISH break apart probe (two colours) | ||
| probes label two parts of a (normal) gene; the two markers straddle (common) break points | | probes label two parts of a (normal) gene; the two markers straddle (common) break points | ||
| gene fragmentation consistent with translocation; one may find: loss of part of the gene, gene duplication | | gene fragmentation consistent with translocation; one may find: loss of part of the gene, gene duplication | ||
| Line 88: | Line 88: | ||
| can detect translocations - without knowing the specific fusion product | | can detect translocations - without knowing the specific fusion product | ||
|- | |- | ||
| ISH fusion probe (two colours) | | Interphase ISH fusion probe (two colours) | ||
| probes label different genes (that are not adjacent) | | probes label different genes (that are not adjacent) | ||
| translocation involving the two genes labeled; one may find: gene duplication, loss of a gene) | | translocation involving the two genes labeled; one may find: gene duplication, loss of a gene) | ||
| Line 94: | Line 94: | ||
| can detect one specific translocation | | can detect one specific translocation | ||
|- | |- | ||
| ISH probe (one colour) | | Interphase ISH probe (one colour) | ||
| probe | | probe labels one region (gene) | ||
| gene duplication, loss of a gene | | gene duplication, loss of a gene | ||
| $$ | | $$ | ||
| Line 105: | Line 105: | ||
| $$$ | | $$$ | ||
| gives the "big picture" view of all the (nuclear) DNA | | gives the "big picture" view of all the (nuclear) DNA | ||
|- | |||
| Metaphase ISH probe (one colour / two colours) | |||
| probe labels one region (one colour) ''or'' probes label two parts of a (normal) gene (two colours) ''or'' probes label different genes (two colours) | |||
| gene duplication, gene loss, translocations | |||
| $$$$ | |||
| can be thought of as interphase ISH + simulateous karyotyping | |||
|} | |} | ||