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Difference between revisions of "Cytogenetics"
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***The detail of an ideogram is given in "band levels"; a 850 band ideogram has a higher resolution than a 400 band ideogram. The typical range for band level is 300-850. The band level, for a given specimen, is determined by an empirical standard and based on the number of bands an observer sees in a subset of the chromosomes.<ref name=pmid8291552>{{Cite journal | last1 = Welborn | first1 = JL. | last2 = Welborn | first2 = R. | title = Banding resolution of human chromosomes: a method of accuracy and simplicity. | journal = Am J Med Genet | volume = 47 | issue = 8 | pages = 1180-3 | month = Dec | year = 1993 | doi = 10.1002/ajmg.1320470810 | PMID = 8291552 }} | ***The detail of an ideogram is given in "band levels"; a 850 band ideogram has a higher resolution than a 400 band ideogram. The typical range for band level is 300-850. The band level, for a given specimen, is determined by an empirical standard and based on the number of bands an observer sees in a subset of the chromosomes.<ref name=pmid8291552>{{Cite journal | last1 = Welborn | first1 = JL. | last2 = Welborn | first2 = R. | title = Banding resolution of human chromosomes: a method of accuracy and simplicity. | journal = Am J Med Genet | volume = 47 | issue = 8 | pages = 1180-3 | month = Dec | year = 1993 | doi = 10.1002/ajmg.1320470810 | PMID = 8291552 }} | ||
</ref> | </ref> | ||
*Typically done with karyotyping software (e.g. Ikaros from MetaSystems<ref>URL: [http://www.metasystems-international.com/index.php?option=com_content&view=article&id=46:ikaros-general&catid=34:ikarosisis&Itemid=119 http://www.metasystems-international.com/index.php?option=com_content&view=article&id=46:ikaros-general&catid=34:ikarosisis&Itemid=119]. Accessed on: 12 May 2011.</ref>) | *Typically done with karyotyping software (e.g. ''Ikaros'' from ''MetaSystems''<ref>URL: [http://www.metasystems-international.com/index.php?option=com_content&view=article&id=46:ikaros-general&catid=34:ikarosisis&Itemid=119 http://www.metasystems-international.com/index.php?option=com_content&view=article&id=46:ikaros-general&catid=34:ikarosisis&Itemid=119]. Accessed on: 12 May 2011.</ref>). | ||
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Notes: | Notes: | ||
*Quinacrine dye - AT-rich regions brighter than GC-rich regions. | *Quinacrine dye - AT-rich regions brighter than GC-rich regions. | ||
===Virtual karyotyping=== | |||
*An evolving technique based on SNP microarrays<ref name=pmid20736741>{{Cite journal | last1 = Alvarez | first1 = K. | last2 = Kash | first2 = SF. | last3 = Lyons-Weiler | first3 = MA. | last4 = Kim | first4 = HJ. | last5 = Peterson | first5 = LE. | last6 = Mathai | first6 = B. | last7 = Hagenkord | first7 = JM. | last8 = Monzon | first8 = FA. | title = Reproducibility and performance of virtual karyotyping with SNP microarrays for the detection of chromosomal imbalances in formalin-fixed paraffin-embedded tissues. | journal = Diagn Mol Pathol | volume = 19 | issue = 3 | pages = 127-34 | month = Sep | year = 2010 | doi = 10.1097/PDM.0b013e3181d527c5 | PMID = 20736741 }} | |||
</ref> or comparative genomic hybridization (CGH). | |||
==ISH== | ==ISH== | ||