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Difference between revisions of "Hodgkin lymphoma"
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==Microscopic== | ==Microscopic== | ||
Defined by ''Reed-Sternberg cells'' (RSCs). Morphologically similar mononuclear cells are known as Hodgkin cells. RSCs are: | |||
*Large binucleated cell (>= 45 micrometres).<ref name=Ref_PCPBoD8_329>{{Ref PCPBoD8|329}}</ref> | |||
**May be multinucleated. | |||
**May have a horseshoe-like shape. | |||
*[[Macronucleolus]] - approximately the size of a RBC (~8 micrometers). | |||
*Well-defined cell border. | |||
*Abundant cytoplasm. | |||
RSC may show peri-cellular clearing, making the cells appear within a space. These are called lacunar cells (as they are in a "lake"). Apoptotic RSC may show pyknotic nuclei and scant eosinophilic cytoplasm and are sometimes known as "mummified" cells. | |||
===Images (classic HL)=== | |||
<gallery> | <gallery> | ||
Image:Hodgkin_lymphoma_cytology_large.jpg | HL mixed cellularity - cytology. (WC) | Image:Hodgkin_lymphoma_cytology_large.jpg | HL mixed cellularity - cytology. (WC) | ||
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*CD30 Reed-Sternberg cells (RSCs) +ve ~98% | *CD30 Reed-Sternberg cells (RSCs) +ve ~98% | ||
*CD15 Reed-Sternberg cells +ve ~80%, stains neutrophils. | *CD15 Reed-Sternberg cells +ve ~80%, stains neutrophils. | ||
*MUM1 +ve | |||
*CD45 '''often negative''' in RSCs. | *CD45 '''often negative''' in RSCs. | ||
*CD20 may stain RSCs. | *CD20 may stain RSCs (usually negative, but can be weak). | ||
*PAX5 +ve.<ref name=Ref_APBR683>{{Ref APBR|683}}</ref> | *PAX5 +ve.<ref name=Ref_APBR683>{{Ref APBR|683}}</ref> | ||
Additional - for completeness: | Additional - for completeness: | ||
*CD3 (T lymphocytes) | *CD3 (T lymphocytes) | ||
*40% are EBV positive. | |||
NLPHL IHC '''differs''' from the classical HL:<ref name=Ref_APBR683>{{Ref APBR|683}}</ref> | NLPHL IHC '''differs''' from the classical HL:<ref name=Ref_APBR683>{{Ref APBR|683}}</ref> | ||
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*CD10 +ve. | *CD10 +ve. | ||
*Bcl-6 +ve. | *Bcl-6 +ve. | ||
*[[EMA]] +ve | *[[EMA]] +ve (40-50%) | ||
*CD30 -ve | *CD30 -ve | ||
*CD15 -ve. | *CD15 -ve. | ||