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==Hale's colloidal iron stain== | ==Hale's colloidal iron stain== | ||
{{:Hale's colloidal iron stain}} | |||
=== | ==von Kossa stain== | ||
* | ===General=== | ||
*Type of silver stain.<ref name=pmid8360080>{{Cite journal | last1 = Rungby | first1 = J. | last2 = Kassem | first2 = M. | last3 = Eriksen | first3 = EF. | last4 = Danscher | first4 = G. | title = The von Kossa reaction for calcium deposits: silver lactate staining increases sensitivity and reduces background. | journal = Histochem J | volume = 25 | issue = 6 | pages = 446-51 | month = Jun | year = 1993 | doi = | PMID = 8360080 }}</ref> | |||
===Use=== | ===Use=== | ||
*Look for calcium. | *Look for calcium. | ||
*Actually stains phosphates and carbonates as a surrogate for calcium. | |||
===Interpretation=== | ===Interpretation=== | ||
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Image: Lung adenocarcinoma - Diff-Quik -- high mag.jpg | [[Pulmonary_cytopathology#Adenocarcinoma|Lung adenocarcinoma]] - DQ - high mag. (WC) | Image: Lung adenocarcinoma - Diff-Quik -- high mag.jpg | [[Pulmonary_cytopathology#Adenocarcinoma|Lung adenocarcinoma]] - DQ - high mag. (WC) | ||
Image: Lung adenocarcinoma - Diff-Quik -- very high mag.jpg | Lung adenocarcinoma - DQ - very high mag. (WC) | Image: Lung adenocarcinoma - Diff-Quik -- very high mag.jpg | Lung adenocarcinoma - DQ - very high mag. (WC) | ||
Image: Lung small cell carcinoma - Diff-Quik -- very high mag.jpg | Lung SmCC - DQ - very high mag. (WC) | |||
Image: Lung small cell carcinoma - Diff-Quik -- extremely high mag.jpg | Lung SmCC - DQ - extremely high mag. (WC) | |||
</gallery> | </gallery> | ||
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===General=== | ===General=== | ||
*Used in [[medical liver disease|medical liver]] biopsies - | *Used in [[medical liver disease|medical liver]] biopsies - stains sulfhydrl groups and identifies: | ||
**Copper-associated protein. | |||
**Elastin. | |||
**[[Hepatitis B]] surface antigen.<ref name=pmid7822848>{{Cite journal | last1 = Ghosh | first1 = AK. | last2 = Dasgupta | first2 = A. | last3 = Raha | first3 = K. | last4 = Jana | first4 = A. | last5 = Majumdar | first5 = DN. | title = Hepatic histology in chronic liver disease in hepatitis B surface antigen positive cases. | journal = J Indian Med Assoc | volume = 92 | issue = 10 | pages = 333-5 | month = Oct | year = 1994 | doi = | PMID = 7822848 }}</ref> | |||
===Interpretation=== | ===Interpretation=== | ||
Features:<ref>URL: [http://www.nottingham.ac.uk/pathology/protocols/shikata.html http://www.nottingham.ac.uk/pathology/protocols/shikata.html]. Accessed on: 24 January 2011.</ref> | Features:<ref>URL: [http://www.nottingham.ac.uk/pathology/protocols/shikata.html http://www.nottingham.ac.uk/pathology/protocols/shikata.html]. Accessed on: 24 January 2011.</ref> | ||
* | *Dark purple/brown = elastin fibres, HBsAg and copper-associated protein | ||
*Light purple = background | |||
*Light purple = background | *Red = nuclei (only if counter-stain used) | ||
* | |||
==Gömöri Trichrome stain== | ==Gömöri Trichrome stain== | ||
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*Yellow = muscle, fibrin, [[erythrocytes]]. | *Yellow = muscle, fibrin, [[erythrocytes]]. | ||
*Green/brown = nuclei. | *Green/brown = nuclei. | ||
====Images==== | |||
<gallery> | |||
Image: Normal visceral pleura of lung - Miller -- high mag.jpg | Miller stain showing lung tissue. (WC) | |||
</gallery> | |||
=See also= | =See also= |
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