Difference between revisions of "Libre Pathology talk:Study Group"

1. List three differences between DNA and RNA.

2. What are the three stop codons?

3. Where does transcription begin?

4. List three enzymes necessary for transcription and their function.  

5. List and describe four post transcription modifications of RNA.

6. List three differences between somatic and germline mutations.  

7. What is the difference between a missense and a non-sense mutation?

8. Define a frameshift mutation.  

9. Why are inversion mutations difficult to detect?

10. Describe the potential sequelae of a translocation mutation.  

1. Translate the following: c.1524_1527delCGTA.

2. List 5 features of SNPs.

3. Define a regulatory SNP and a synonymous SNP?

4. What is the difference between a microstalellite and a minisattelite?

5. Describe Hardy-Weinberg Equilibrium?

6. What factors can disrupt the H-W equilibrium?

7. What is linkage disequilibrium?

1. What are the three major steps of PCR?

2. What is the hallmark of PCR?

3.    What factors affect the method of genotyping chosen?

4.    Define sensitivity, specificity, positive predictive value and negative predictive value.  

5.    Define reproducibility and accuracy of an analytical test.  

6.    Describe briefly Sanger sequencing.

7.    Describe briefly how Taqman automated genotyping is used for allele detection.  

8.    How are DNA microarrays used to identify drug disposition or responses?

1.    Describe the procedure for submitting FFPE slides for KRAS for colorectal cancer.

2.    Compare and contrast uniplex versus multiplex genotyping.  

3.    Compare and contrast conventional vs massively parallel sequencing.  

4.    What is multiplex ligation-dependent ligation (MLPA)?

5.    What is fragment analysis?

6.  Compare and contrast RT-PCR vs qRTPCR.

7.  What is MSI?

8.  What is methylation analysis?

1.  What are the four test features required to be documented by the CLIA?

2.  What are "in vitro diagnostics" vs "laboratory developed tests"?

3.  What does validation mean?  

4.  What are the four performance characteristics that need to be verified for FDA cleared/approved tests?

5.  What are the six performance characteristics that need to be verified for FDA cleared LDTs or modified FDA cleared/approved tests?

1.  List the components of a molecular pathology report.

2.  Define analytical sensitivity and clinical sensitivity.  

3.  What should be said in a report of a molecular test on a patient for residual disease if no previous positive assay was confirmed?

4.  Define ammended report versus addendum report.

5.  Whose responsibility is it to sythesize the test results with other clinico-pathological information?

6.  How long are cytogenetic reports required to be kept by CAP?

7,  What is the recommended process to use test results if an assay is not yet validated for clinical use?

8.  Give three examples of "grey areas" which warrant discretion of professionals involved to use a non-validated test?

9.  What reference standard is available for gene nomenclature?

10. Create a table of the most common gene rearrangements associated with heme and soft tissue diseases.  

11. What is a "DNA fingerprint" and what can it be used for?

 

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I think the template could use some re-formating... but the idea should work. I got the template [http://en.wikipedia.org/wiki/Template:Hidden from here]. There are [http://en.wikiversity.org/wiki/Test_and_Quiz similar things here].