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Difference between revisions of "Anatomical pathology laboratory processes"
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Anatomical pathology laboratory processes (view source)
Revision as of 21:08, 27 December 2023
, 21:08, 27 December 2023no edit summary
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#Staining. | #Staining. | ||
#*Routinue stains. | #*Routinue stains. | ||
#*Special stains. | #*[[Special stains]]. | ||
#*[[Immunostains]]. | #*[[Immunostains]]. | ||
#Case assembly and distribution. | #Case assembly and distribution. | ||
#Diagnosis. | #Diagnosis. | ||
#*Pathologist. | #*Pathologist +/- pathology [[resident]]. | ||
#**Tasks: | #**Tasks: | ||
#***More tissue. | #***More tissue. | ||
#***Levels/deepers. | #***[[Levels]]/deepers. | ||
#***Special [[stains]]. | #***Special [[stains]]. | ||
#***Immunostains. | #***Immunostains. | ||
#***Write report. | #***[[Sign out|Write report]]. | ||
#****Consult other pathologists - in house | #****Consult other pathologists - in house (internal consult) or outside (external consult). | ||
#****Discuss with clinician. | #****Discuss with clinician. | ||
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*Specimen descriptors: | *Specimen descriptors: | ||
**How many parts, i.e. how many containers. | **How many parts, i.e. how many containers. | ||
***One container = at least | ***One container = at least one [[diagnosis]]. | ||
***Anatomical site of the parts, e.g. kidney biopsy. | ***Anatomical site of the parts, e.g. kidney biopsy. | ||
The above information is all contained on the requisition. | The above information is all contained on the requisition. | ||
===Accessioning=== | ===Accessioning=== | ||
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*Tissue is cut from the block. | *Tissue is cut from the block. | ||
**Pieces of tissue sent to the pathologist are tracked as "levels". | **Pieces of tissue sent to the pathologist are tracked as "levels". | ||
**Who cut the tissue is tracked. | **Who cut the tissue is tracked. | ||
Notes: | |||
*The thickness of the sections can make a difference in the interpretation.<ref name=pmid1915756>{{Cite journal | last1 = Otto | first1 = MJ. | last2 = Löw | first2 = O. | last3 = Schneider | first3 = A. | title = The nominal section thickness--importance of their correction for morphometry. | journal = Exp Pathol | volume = 42 | issue = 3 | pages = 129-36 | month = | year = 1991 | doi = | PMID = 1915756 }}</ref> | |||
**Routine sections are cut at 3-4 micrometres. | |||
***3 micrometres is considered ideal for GI biopsies and prostate biopsies.<ref>LE. 20 January 2015.</ref> | |||
***4 micrometres is a recommendation for immunostains.<ref>{{cite journal |authors=Magaki S, Hojat SA, Wei B, So A, Yong WH |title=An Introduction to the Performance of Immunohistochemistry |journal=Methods Mol Biol |volume=1897 |issue= |pages=289–298 |date=2019 |pmid=30539453 |pmc=6749998 |doi=10.1007/978-1-4939-8935-5_25 |url=}}</ref> | |||
***Sections for [[Congo red|Congo red]] are usually ~10 micrometres. | |||
===Staining=== | ===Staining=== | ||
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*[[Fixation]]. | *[[Fixation]]. | ||
*[[Laboratory information system]]. | *[[Laboratory information system]]. | ||
==References== | |||
{{Reflist|1}} | |||
==External links== | |||
*[http://protocolsonline.com/histology/sample-preparation/paraffin-processing-of-tissue/ Sample preparation (protocolsonline.com)]. | |||
[[Category:Quality]] | [[Category:Quality]] | ||