Difference between revisions of "Stains"

Stains (view source)

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 ===Use===
 *Stain for melanin.
+**Displays melanin - whether it be in melanocytes, keratinocytes or melanophages.
+**IHC stains, i.e. ''Melan A, SOX10 or MITF'' are preferable for displaying ''melanocytes''.
 **Used to differentiate brown pigments (lipofuscin, hemosiderin, melanin).<ref>URL: [http://education.vetmed.vt.edu/curriculum/VM8054/labs/Lab2/Examples/exfontana.htm http://education.vetmed.vt.edu/curriculum/VM8054/labs/Lab2/Examples/exfontana.htm]. Accessed on: 5 May 2010.</ref>
-***Many pathologists prefer IHC, i.e. ''Melan A'' over this stain.
+**Used to document Minocycline type II drug induced pigment deposition
+***Minocycline pigment, Type II will stain with both the Fontana-Masson stain AND the Perls iron stain <ref>Acta Dermatovenerol Croat. 2009;17(2):123-6. Minocycline-induced skin pigmentation: an update. Geria AN1, Tajirian AL, Kihiczak G, Schwartz RA.</ref>
+**Used in the differential diagnosis of hypomelanosis
+***Idiopathic hypomelanosis will demonstrate ONLY loss of melanin with the Fontana-Masson stain - melanocytes will not be absent with the Melan A stain.
+***Vitiligo will show loss of melanin AND loss of melanocytes with a Melan A stain.
 Image:
 *[http://education.vetmed.vt.edu/curriculum/VM8054/labs/Lab2/Examples/exfontana.htm Fontana-Masson stain (vetmed.vt.edu)].