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Difference between revisions of "Immunohistochemical staining"
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Immunohistochemical staining (view source)
Revision as of 03:03, 1 September 2012
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This is an evolving area in pathology that has been ignored for a surprisingly long time. | This is an evolving area in pathology that has been ignored for a surprisingly long time. | ||
It is touched upon the in the ''[[quality]]'' article in the ''[[Quality#Immunohistochemistry|immunohistochemistry]]'' section. | It is touched upon the in the ''[[quality]]'' article in the ''[[Quality#Immunohistochemistry|immunohistochemistry]]'' section. | ||
==Interpretation== | |||
To determine whether a stain is (1) done correctly, and (2) positive, one needs to know: | |||
#What tissues it stains: | |||
#*Tumour. | |||
#*Normal tissue. | |||
#How it stains the various tissues: | |||
#*Patchy. | |||
#*Diffuse. | |||
#Where it stains the various tissued: | |||
#*Nucleus. | |||
#*Cytoplasm. | |||
#*Membrane. | |||
#*A combination of the above. | |||
Generally, interpretations are subjective, and this is especially true when the staining is weak and focal. In other words, "... your weak [positive] stain might be somebody else’s negative."<ref>URL: [http://bitesizebio.com/articles/immunohistochemistry-getting-the-stain-you-want/ http://bitesizebio.com/articles/immunohistochemistry-getting-the-stain-you-want/]. Accessed on: 1 September 2012.</ref> | Generally, interpretations are subjective, and this is especially true when the staining is weak and focal. In other words, "... your weak [positive] stain might be somebody else’s negative."<ref>URL: [http://bitesizebio.com/articles/immunohistochemistry-getting-the-stain-you-want/ http://bitesizebio.com/articles/immunohistochemistry-getting-the-stain-you-want/]. Accessed on: 1 September 2012.</ref> | ||