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Difference between revisions of "Stains"
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redo ref WMSP
m (→Where to start...: re-order notes) |
m (redo ref WMSP) |
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***Histoplasma = black, round balls. | ***Histoplasma = black, round balls. | ||
Notes: ''GMS'' is "Grocott's methenamine Silver" according to WMSP.<ref>WMSP | Notes: ''GMS'' is "Grocott's methenamine Silver" according to WMSP.<ref name=Ref_WMSP682>{{Ref WMSP|682}}</ref> | ||
==Acid-fast bacilli stains== | ==Acid-fast bacilli stains== | ||
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===Interpretation=== | ===Interpretation=== | ||
*Carmine with metanil yellow and Weigert's Hematoxylin:<ref>WMSP | *Carmine with metanil yellow and Weigert's Hematoxylin:<ref name=Ref_WMSP678>{{Ref WMSP|678}}</ref> | ||
**Blue/black = nucleus. | **Blue/black = nucleus. | ||
**Yellow = background. | **Yellow = background. | ||
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===General=== | ===General=== | ||
*Stains acidic mucin (pH=2.5); '''A'''lcian blue = '''A'''cidic. | *Stains acidic mucin (pH=2.5); '''A'''lcian blue = '''A'''cidic. | ||
**A variant uses pH=1.0.<ref>WMSP | **A variant uses pH=1.0.<ref name=Ref_WMSP682>{{Ref WMSP|682}}</ref> | ||
===Use=== | ===Use=== | ||
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===Interpretation=== | ===Interpretation=== | ||
*Blue is positive.<ref name= | *Blue is positive.<ref name=Ref_WMSP682>{{Ref WMSP|682}}</ref> | ||
Notes: | Notes: | ||
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===Interpretation=== | ===Interpretation=== | ||
*Black = calcium.<ref>WMSP | *Black = calcium.<ref name=Ref_WMSP682>{{Ref WMSP|682}}</ref> | ||
==Toluidine blue== | ==Toluidine blue== | ||