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K27M staining in a diffuse glioma of the midline.

H3F3A (K27M), often abbreviated K27M or H3.3 K27M is a mutation-specific antibody detecting the K27M mutation in tumor nuclei.[1]

Positive staining

  • Nuclear
    • Approx 30% of pediatric glioma.
    • Approx 80% of diffuse intrinsic pontine glioma (DIPG).


  • The antibody does not detect other H3F3A mutations (ie. G34V or G34R).

Normal tissues

  • Absent

See also


  1. Venneti, S.; Santi, M.; Felicella, MM.; Yarilin, D.; Phillips, JJ.; Sullivan, LM.; Martinez, D.; Perry, A. et al. (Nov 2014). "A sensitive and specific histopathologic prognostic marker for H3F3A K27M mutant pediatric glioblastomas.". Acta Neuropathol 128 (5): 743-53. doi:10.1007/s00401-014-1338-3. PMID 25200322.