DNA sequence comparison

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DNA sequence comparison is component of molecular pathology.

DNA sequencing redirect here.

Nomenclature

This is still somewhat in flux.[1]

Data format

FASTA: This contains just DNA sequence. The first line starts with '>' and contains the record name.

FASTQ: This contains DNA sequence data with quality scores: The quality scores are indicated by defined characters.

Basic local alignment search tool

Using BLAST

  1. Go to BLAST web site ( http://blast.ncbi.nlm.nih.gov/Blast.cgi ).
  2. Enter sequence.
  3. Enter consensus sequence (can be found with OMIM).
  4. Hit enter.

See also

References

  1. ↑ Ogino S, Gulley ML, den Dunnen JT, Wilson RB (February 2007). "Standard mutation nomenclature in molecular diagnostics: practical and educational challenges". J Mol Diagn 9 (1): 1–6. doi:10.2353/jmoldx.2007.060081. PMC 1867422. PMID 17251329. http://moldiag.highwire.org/cgi/content/full/9/1/1#B6.

External links

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