Difference between revisions of "Trophoblast"

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**Clear cytoplasm.  
**Clear cytoplasm.  
**Single uniform nucleus.
**Single uniform nucleus.
===Implantation site===
*Intermediate trophoblast.
====IHC====
Features:<ref name=pmid1694585>{{cite journal |author=Yeh IT, O'Connor DM, Kurman RJ |title=Intermediate trophoblast: further immunocytochemical characterization |journal=Mod. Pathol. |volume=3 |issue=3 |pages=282–7 |year=1990 |month=May |pmid=1694585 |doi= |url=}}</ref>
*hPL (human placental lactogen) +ve.
*beta-hCG -ve.
*Keratin +ve.
*EMA +ve in 2nd & 3rd trimester.
Notes:
*Cytotrophoblast and syncytiotrophoblast - keratin +ve.<ref name=pmid1694585/>


==Age of villi==
==Age of villi==

Revision as of 12:56, 22 July 2010

Chorionic villi are part of a normal pregnancy. They are derived from the conceptus, i.e. NOT maternal.

Tumours arising from the trophoblast are dealt with in gestational trophoblastic disease.

Chorionic villi 101

  • Maternal blood is around villi.
  • Fetal blood (nucleated (fetal) RBCs) in the villi.

Basic histology

  • Outer layer of villus: syncytiotrophoblasts.
  • Beneath syncytiotrophoblast (and closer to fetus) cytotrophoblasts.

Chorionic villi 102

  • Syncytiotrophoblasts (eosinophilic cytoplasm with vacuoles (contain hCG), multiple hyperchromatic nuclei).
    • Large + many irreg. or lobular hyperchromatic nuclei.
    • Eosinophilic vacuolated cytoplasm (contains hCG).
    • Closest to mom - covers cytotrophoblast.[1]
  • Cytotrophoblasts (polygonal shape, distinct borders, clear cytoplasm, in cords, single nucleus).
    • Polygonal shaped cells in cords/masses.
    • Distinct cell borders.
    • Covered by syncytiobrophoblast[2] - closer to fetus than syncytiotrophoblasts.
    • Clear cytoplasm.
    • Single uniform nucleus.

Implantation site

  • Intermediate trophoblast.

IHC

Features:[3]

  • hPL (human placental lactogen) +ve.
  • beta-hCG -ve.
  • Keratin +ve.
  • EMA +ve in 2nd & 3rd trimester.

Notes:

  • Cytotrophoblast and syncytiotrophoblast - keratin +ve.[3]

Age of villi

Young villi

Old villi

  • Blood vessels present and at the periphery of villus - contain nucleated (fetal) RBCs.
  • Syncytial knots.

See also

References

  1. http://upload.wikimedia.org/wikipedia/commons/4/45/Gray37.png
  2. http://upload.wikimedia.org/wikipedia/commons/4/45/Gray37.png
  3. 3.0 3.1 Yeh IT, O'Connor DM, Kurman RJ (May 1990). "Intermediate trophoblast: further immunocytochemical characterization". Mod. Pathol. 3 (3): 282–7. PMID 1694585.