Difference between revisions of "Papanicolaou stain"

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*Can be thought of as the [[H&E stain|H&E]] of [[cytopathology]].
*Can be thought of as the [[H&E stain|H&E]] of [[cytopathology]].
**It is a modified [[H&E stain]].
**It is a modified [[H&E stain]].
*Specimens are fixed in ethanol.
*Specimens are [[fixation|fixed]] in ethanol.
*Good for seeing nuclear detail.<ref name=pmid16295430/>
*Good for seeing nuclear detail.<ref name=pmid16295430/>
*Out-of-focus cytoplasm is translucent; allows one to focus overlapped cells in different planes.
*Out-of-focus cytoplasm is translucent; allows one to focus overlapped cells in different planes.
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===Images===
===Images===
<gallery>
<gallery>
Image:Low grade squamous intraepithelial lesion.jpg|LSIL - Pap stain. (WC)
Image:Low grade squamous intraepithelial lesion.jpg|Cervix - LSIL - Pap stain. (WC)
Image:High-grade squamous intraepithelial lesion.jpg|HSIL - Pap stain. (WC)
Image:High-grade squamous intraepithelial lesion.jpg|Cervix - HSIL - Pap stain. (WC)
</gallery>
Image:Lung small cell carcinoma -- extremely high mag.jpg|Lung - SmCC - Pap stain. (WC)
<gallery>
Image:Urine_citology_urothelial_carcinoma_2.jpg | [[Urine cytology]] - UCC - Pap stain. (WC)
Image:Urine_citology_urothelial_carcinoma_2.jpg | [[Urine cytology]] - Pap stain. (WC)
</gallery>
</gallery>



Latest revision as of 04:17, 24 April 2016

Papanicolaou stain
Stain in short

Low grade squamous intraepithelial lesion.
Abbreviation Pap stain
Similar stains Romanowsky stains
Use the standard stain in cytopathology
Interpretation blue/purple = nucleus, pink/green = cytoplasm, orange = keratin

Papanicolaou stain, abbreviated Pap stain, is a standard stain used in cytopathology.[1] It is a modified H&E stain.

General

  • Can be thought of as the H&E of cytopathology.
  • Specimens are fixed in ethanol.
  • Good for seeing nuclear detail.[1]
  • Out-of-focus cytoplasm is translucent; allows one to focus overlapped cells in different planes.

Use

Interpretation

  • Blue/purple = nucleus.
  • Green/pink = cytoplasm.
  • Orange = keratin.

Images

See also

References

  1. 1.0 1.1 Prabhudesai, NM.; Kulkarni, MB.; Desai, SB.; Borges, AM. (Jul 2004). "Modified H & E staining technique for fine needle aspiration cytology (FNAC) smears.". Indian J Pathol Microbiol 47 (3): 384-6. PMID 16295430.