Difference between revisions of "Papanicolaou stain"

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'''Papanicolaou stain''', abbreviated '''Pap stain''', is a standard [[stain]] used in [[cytopathology]].  It is a modified [[H&E stain]].
{{ Infobox stain
| Name      = {{PAGENAME}}
| Image      = Low grade squamous intraepithelial lesion.jpg
| Width      =
| Caption    = Low grade squamous intraepithelial lesion.
| Abbrev    = Pap stain
| Synonyms  =
| Variants  =     
| Similar    = [[Romanowsky stain]]s     
| Use        = the standard stain in [[cytopathology]]
| Subspecial = 
| Interpret  = blue/purple = nucleus, pink/green = cytoplasm, orange = keratin
| Positive  =
| Negative  =
| Other      =
}}
'''Papanicolaou stain''', abbreviated '''Pap stain''', is a standard [[stain]] used in [[cytopathology]].<ref name=pmid16295430>{{Cite journal  | last1 = Prabhudesai | first1 = NM. | last2 = Kulkarni | first2 = MB. | last3 = Desai | first3 = SB. | last4 = Borges | first4 = AM. | title = Modified H & E staining technique for fine needle aspiration cytology (FNAC) smears. | journal = Indian J Pathol Microbiol | volume = 47 | issue = 3 | pages = 384-6 | month = Jul | year = 2004 | doi =  | PMID = 16295430 }}</ref> It is a modified [[H&E stain]].


==General==
==General==
*Can be thought of as the [[H&E stain|H&E]] of [[cytopathology]].
*Can be thought of as the [[H&E stain|H&E]] of [[cytopathology]].
**It is a modified [[H&E stain]].
**It is a modified [[H&E stain]].
*Specimens are fixed in ethanol.
*Specimens are [[fixation|fixed]] in ethanol.
*Good for seeing nuclear detail.
*Good for seeing nuclear detail.<ref name=pmid16295430/>
*Out-of-focus cytoplasm is translucent; allows one to focus overlapped cells in different planes.
*Out-of-focus cytoplasm is translucent; allows one to focus overlapped cells in different planes.


==Use==
==Use==
*Cytopathology.
*[[Cytopathology]].


==Interpretation==
==Interpretation==
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*Orange = keratin.
*Orange = keratin.


===Image===
===Images===
<gallery>
<gallery>
Image:Urine_citology_urothelial_carcinoma_2.jpg | Pap stain - [[urine cytology]] (WC)
Image:Low grade squamous intraepithelial lesion.jpg|Cervix - LSIL - Pap stain. (WC)
Image:High-grade squamous intraepithelial lesion.jpg|Cervix - HSIL - Pap stain. (WC)
Image:Lung small cell carcinoma -- extremely high mag.jpg|Lung - SmCC - Pap stain. (WC)
Image:Urine_citology_urothelial_carcinoma_2.jpg | [[Urine cytology]] - UCC - Pap stain. (WC)
</gallery>
</gallery>


==See also==
==See also==
*[[Stains]].
*[[Stains]].
==References==
{{Reflist|1}}


[[Category:Stains]]
[[Category:Stains]]

Latest revision as of 04:17, 24 April 2016

Papanicolaou stain
Stain in short

Low grade squamous intraepithelial lesion.
Abbreviation Pap stain
Similar stains Romanowsky stains
Use the standard stain in cytopathology
Interpretation blue/purple = nucleus, pink/green = cytoplasm, orange = keratin

Papanicolaou stain, abbreviated Pap stain, is a standard stain used in cytopathology.[1] It is a modified H&E stain.

General

  • Can be thought of as the H&E of cytopathology.
  • Specimens are fixed in ethanol.
  • Good for seeing nuclear detail.[1]
  • Out-of-focus cytoplasm is translucent; allows one to focus overlapped cells in different planes.

Use

Interpretation

  • Blue/purple = nucleus.
  • Green/pink = cytoplasm.
  • Orange = keratin.

Images

See also

References

  1. 1.0 1.1 Prabhudesai, NM.; Kulkarni, MB.; Desai, SB.; Borges, AM. (Jul 2004). "Modified H & E staining technique for fine needle aspiration cytology (FNAC) smears.". Indian J Pathol Microbiol 47 (3): 384-6. PMID 16295430.