48,460
edits
Difference between revisions of "Immunohistochemical staining"
Jump to navigation
Jump to search
Immunohistochemical staining (view source)
Revision as of 03:03, 1 September 2012
, 03:03, 1 September 2012→Quality control: tweak
(→Quality control: tweak) |
|||
| Line 15: | Line 15: | ||
This is an evolving area in pathology that has been ignored for a surprisingly long time. | This is an evolving area in pathology that has been ignored for a surprisingly long time. | ||
It is touched upon the in the ''[[quality]]'' article in the ''[[Quality#Immunohistochemistry|immunohistochemistry]]'' section. | It is touched upon the in the ''[[quality]]'' article in the ''[[Quality#Immunohistochemistry|immunohistochemistry]]'' section. | ||
==Interpretation== | |||
To determine whether a stain is (1) done correctly, and (2) positive, one needs to know: | |||
#What tissues it stains: | |||
#*Tumour. | |||
#*Normal tissue. | |||
#How it stains the various tissues: | |||
#*Patchy. | |||
#*Diffuse. | |||
#Where it stains the various tissued: | |||
#*Nucleus. | |||
#*Cytoplasm. | |||
#*Membrane. | |||
#*A combination of the above. | |||
Generally, interpretations are subjective, and this is especially true when the staining is weak and focal. In other words, "... your weak [positive] stain might be somebody else’s negative."<ref>URL: [http://bitesizebio.com/articles/immunohistochemistry-getting-the-stain-you-want/ http://bitesizebio.com/articles/immunohistochemistry-getting-the-stain-you-want/]. Accessed on: 1 September 2012.</ref> | Generally, interpretations are subjective, and this is especially true when the staining is weak and focal. In other words, "... your weak [positive] stain might be somebody else’s negative."<ref>URL: [http://bitesizebio.com/articles/immunohistochemistry-getting-the-stain-you-want/ http://bitesizebio.com/articles/immunohistochemistry-getting-the-stain-you-want/]. Accessed on: 1 September 2012.</ref> | ||