Difference between revisions of "Flow cytometry"

Jump to navigation Jump to search
1,141 bytes added ,  11:10, 27 December 2012
more
(create)
 
(more)
 
(2 intermediate revisions by the same user not shown)
Line 3: Line 3:
==Principles==
==Principles==
# Label cells with fluorescent antibodies.
# Label cells with fluorescent antibodies.
# Passed through a channel one-at-a-time (using ''hydrodynamic focusing''<ref>URL: [http://www.med.umich.edu/flowcytometry/InitialTraining/lessons/lesson1/index.htm http://www.med.umich.edu/flowcytometry/InitialTraining/lessons/lesson1/index.htm]. Accessed on: 5 August 2010.</ref> -- interrogated with a laser light.
# Passed through a channel one-at-a-time (using ''hydrodynamic focusing''<ref>URL: [http://www.med.umich.edu/flowcytometry/InitialTraining/lessons/lesson1/index.htm http://www.med.umich.edu/flowcytometry/InitialTraining/lessons/lesson1/index.htm]. Accessed on: 5 August 2010.</ref>) and interrogated with a laser light.
 
===Scatter of light===
Types:
#Forward scatter (FS) -- indicative of cell size.
#Side scatter (SS) -- indicative of cell complexity (granularity).
 
====Scatter in a table====
Selected white blood cells & scatter:<ref>URL: [http://www.med.umich.edu/flowcytometry/InitialTraining/lessons/lesson1/images/blood.gif http://www.med.umich.edu/flowcytometry/InitialTraining/lessons/lesson1/images/blood.gif]. Accessed on: 5 August 2010.</ref>
{| class="wikitable"
!
! Forward scatter
! Side scatter
|-
| PMNs
| high
| very high
|-
| Monocytes
| high
| moderate/low
|-
| Lymphocytes
| low
| low
|}
 
==Practical considerations==
*Tissue must be fresh, i.e. cannot be [[formalin]] fixed.
**Viable material may be salvaged from the centre of a large specimen placed in formalin for a short time.
*2 mm x 2 mm x 2 mm piece of tissue is sufficient.
**1 mm<sup>3</sup> is considered the minimum by Mayo Medical Laboratories.<ref>URL: [http://www.mayomedicallaboratories.com/test-catalog/Specimen/19499 http://www.mayomedicallaboratories.com/test-catalog/Specimen/19499]. Accessed on: 27 December 2012.</ref>
 
==Interpretation==
:See ''[[Cytometry]]''.


==See also==
==See also==
48,453

edits

Navigation menu