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Difference between revisions of "Amyloid"
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→Classification of amyloid - differentiation: tweak
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===Biochemical techniques=== | ===Biochemical techniques=== | ||
*Pre-treated with potassium permanganate, to obliterate congo red reactivity, '''''was''''' though be definitive for AA amyloidosis;<ref name=pmid495695>{{cite journal |author=van Rijswijk MH, van Heusden CW |title=The potassium permanganate method. A reliable method for differentiating amyloid AA from other forms of amyloid in routine laboratory practice |journal=Am. J. Pathol. |volume=97 |issue=1 |pages=43–58 |year=1979 |month=October |pmid=495695 |pmc=2042379 |doi= |url=}}</ref> this is now generally considered to be unreliable.<ref name=pmid20367306>{{Cite journal | last1 = Picken | first1 = MM. | title = Amyloidosis-where are we now and where are we heading? | journal = Arch Pathol Lab Med | volume = 134 | issue = 4 | pages = 545-51 | month = Apr | year = 2010 | doi = 10.1043/1543-2165-134.4.545 | PMID = 20367306 }}</ref> | *Pre-treated with potassium permanganate, to obliterate congo red reactivity, '''''was''''' though be definitive for AA amyloidosis;<ref name=pmid495695>{{cite journal |author=van Rijswijk MH, van Heusden CW |title=The potassium permanganate method. A reliable method for differentiating amyloid AA from other forms of amyloid in routine laboratory practice |journal=Am. J. Pathol. |volume=97 |issue=1 |pages=43–58 |year=1979 |month=October |pmid=495695 |pmc=2042379 |doi= |url=}}</ref> this is now generally considered to be unreliable.<ref name=pmid20367306>{{Cite journal | last1 = Picken | first1 = MM. | title = Amyloidosis-where are we now and where are we heading? | journal = Arch Pathol Lab Med | volume = 134 | issue = 4 | pages = 545-51 | month = Apr | year = 2010 | doi = 10.1043/1543-2165-134.4.545 | PMID = 20367306 }}</ref> | ||
===Immunohistochemistry=== | ===Immunohistochemistry=== | ||
*[[IHC]] may be useful for the subclassification of amyloidoses.<ref name=pmid19756621>{{cite journal |author=Röcken C |title=[Update on immunohistological classification of amyloidoses] |language=German |journal=Pathologe |volume=30 Suppl 2 |issue= |pages=121–3 |year=2009 |month=December |pmid=19756621 |doi=10.1007/s00292-009-1183-7 |url=}}</ref> | *[[IHC]] may be useful for the subclassification of amyloidoses.<ref name=pmid19756621>{{cite journal |author=Röcken C |title=[Update on immunohistological classification of amyloidoses] |language=German |journal=Pathologe |volume=30 Suppl 2 |issue= |pages=121–3 |year=2009 |month=December |pmid=19756621 |doi=10.1007/s00292-009-1183-7 |url=}}</ref> | ||
**The reliability of IHC for the subclassification of amyloidoses is thought to be low.<ref name=pmid18181665>{{Cite journal | last1 = Solomon | first1 = A. | last2 = Murphy | first2 = CL. | last3 = Westermark | first3 = P. | title = Unreliability of immunohistochemistry for typing amyloid deposits. | journal = Arch Pathol Lab Med | volume = 132 | issue = 1 | pages = 14; author reply 14-5 | month = Jan | year = 2008 | doi = 10.1043/1543-2165(2008)132[14b:IR]2.0.CO;2 | PMID = 18181665 | url = http://www.archivesofpathology.org/doi/pdf/10.1043/1543-2165%282008%29132%5B14b%3AIR%5D2.0.CO%3B2 }}</ref> | **The reliability of IHC for the subclassification of amyloidoses is thought to be low.<ref name=pmid18181665>{{Cite journal | last1 = Solomon | first1 = A. | last2 = Murphy | first2 = CL. | last3 = Westermark | first3 = P. | title = Unreliability of immunohistochemistry for typing amyloid deposits. | journal = Arch Pathol Lab Med | volume = 132 | issue = 1 | pages = 14; author reply 14-5 | month = Jan | year = 2008 | doi = 10.1043/1543-2165(2008)132[14b:IR]2.0.CO;2 | PMID = 18181665 | url = http://www.archivesofpathology.org/doi/pdf/10.1043/1543-2165%282008%29132%5B14b%3AIR%5D2.0.CO%3B2 }}</ref><ref name=pmid11447744>{{cite journal |author=Murphy CL, Eulitz M, Hrncic R, ''et al.'' |title=Chemical typing of amyloid protein contained in formalin-fixed paraffin-embedded biopsy specimens |journal=Am. J. Clin. Pathol. |volume=116 |issue=1 |pages=135–42 |year=2001 |month=July |pmid=11447744 |doi=10.1309/TWBM-8L4E-VK22-FRH5 |url=}}</ref> | ||
***Extreme caution is advised when interpreting IHC results. | |||
Possibly useful immunostains: | Possibly useful immunostains: | ||