P63
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p63 is a commonly used immunostain. p63, like most other "p" stains, is a nuclear stain.
| P63 | |
|---|---|
| Immunostain in short | |
 p63 staining in squamous epithelium with dysplasia. | |
| Similar stains | p40 (more specific for squamous cell carcinoma) |
| Use | breast pathology (invasion vs. in situ), prostate pathology (HGPIN vs. cancer), lung (adenocarcinoma vs. squamous) |
| Normal staining pattern | nuclear staining |
| Positive | squamous cell carcinoma, urothelial carcinoma |
| Negative | prostate carcinoma, most lung adenocarcinoma, breast carcinoma |
Pattern
- Nuclear staining.
- Stains basal cells in a normal squamous epithelium.
- Superficial cells are typically negative.
- In dysplasia, the staining is seen more superficially,[1] as one might expect as most squamous carcinomas are positive for p63.
Note:
- Cytoplasmic staining suggestive of muscle differentiation - seen in rhabdomyosarcoma.[2]
Staining
Positive - common use
- Marker of squamous cell carcinoma.
- Urothelial carcinoma.[3]
- Small cell carcinoma of the lung ~80% of cases.[4]
Invasive versus in situ
Thresholding (invasive vs. pre-invasive):
- Prostate basal cell marker.
- Breast myoepithelial cell marker.
Negative
Negative p63 staining in a poorly differentiated carcinoma.
- Malignant mesothelioma.[5]
- Breast carcinoma.
- Prostatic carcinoma (rare cases can be P63 positive[6]).
Non-classic tumours
- Di Como et al[7] looked at a large cross-section of tumours.
- Jo and Fletcher[8] did a paper on soft tissue lesions and p63.
See also
References
- ↑ Houghton, O.; McCluggage, WG. (Sep 2009). "The expression and diagnostic utility of p63 in the female genital tract.". Adv Anat Pathol 16 (5): 316-21. doi:10.1097/PAP.0b013e3181b507c6. PMID 19700941.
- ↑ Martin, SE.; Temm, CJ.; Goheen, MP.; Ulbright, TM.; Hattab, EM. (Oct 2011). "Cytoplasmic p63 immunohistochemistry is a useful marker for muscle differentiation: an immunohistochemical and immunoelectron microscopic study.". Mod Pathol 24 (10): 1320-6. doi:10.1038/modpathol.2011.89. PMID 21623385.
- ↑ Lewis, JS.; Ritter, JH.; El-Mofty, S. (Nov 2005). "Alternative epithelial markers in sarcomatoid carcinomas of the head and neck, lung, and bladder-p63, MOC-31, and TTF-1.". Mod Pathol 18 (11): 1471-81. doi:10.1038/modpathol.3800451. PMID 15976812.
- ↑ Au, NH.; Gown, AM.; Cheang, M.; Huntsman, D.; Yorida, E.; Elliott, WM.; Flint, J.; English, J. et al. (Sep 2004). "P63 expression in lung carcinoma: a tissue microarray study of 408 cases.". Appl Immunohistochem Mol Morphol 12 (3): 240-7. doi:10.1097/00129039-200409000-00010. PMID 15551738.
- ↑ Pu, RT.; Pang, Y.; Michael, CW. (Jan 2008). "Utility of WT-1, p63, MOC31, mesothelin, and cytokeratin (K903 and CK5/6) immunostains in differentiating adenocarcinoma, squamous cell carcinoma, and malignant mesothelioma in effusions.". Diagn Cytopathol 36 (1): 20-5. doi:10.1002/dc.20747. PMID 18064689.
- ↑ "Prostate cancer with aberrant diffuse p63 expression: report of a case and review of the literature and morphologic mimics". Arch Pathol Lab Med 137 (9): 1179–84. September 2013. doi:10.5858/arpa.2013-0254-CR. PMID 23991727.
- ↑ Di Como, CJ.; Urist, MJ.; Babayan, I.; Drobnjak, M.; Hedvat, CV.; Teruya-Feldstein, J.; Pohar, K.; Hoos, A. et al. (Feb 2002). "p63 expression profiles in human normal and tumor tissues.". Clin Cancer Res 8 (2): 494-501. PMID 11839669.
- ↑ Jo, VY.; Fletcher, CD. (Nov 2011). "p63 immunohistochemical staining is limited in soft tissue tumors.". Am J Clin Pathol 136 (5): 762-6. doi:10.1309/AJCPXNUC7JZSKWEU. PMID 22031315.