Difference between revisions of "Tissue processing"

From Libre Pathology
Jump to navigation Jump to search
(→‎See also: +tissue floater)
Line 21: Line 21:
*[[Tissue fixation]].
*[[Tissue fixation]].
*[[Molecular pathology]].
*[[Molecular pathology]].
*[[Tissue floater]].


==References==
==References==

Revision as of 23:07, 7 May 2012

Tissue processing is a key component of the histologic examination. This article covers it superficially. It is something a pathologist should have a rudimentary understanding of.

What happens to the tissue

In an ideal world the pathologist gets the tissue fresh and divides it up in the following way:[1]

  1. Formalin.
  2. Electron microscopy.
  3. Molecular genetics.
  4. Cytogenetics.
  5. Flow cytometry.
  6. Tissue banks - institutional, regional, national, international.
  7. Tissue culture.

Tissue processing

Basic process:

  1. Fixation -- formalin.
  2. Embedding -- replace water (in the tissue) with (paraffin) wax - using a series of alcohols.
  3. Sectioning -- cut with microtome ~ 3-10 micrometers thick, routinue is usu. 6-8 micrometers.[2]
  4. Staining.

See also

References

  1. PST. 14 February 2011.
  2. URL: http://library.med.utah.edu/WebPath/HISTHTML/HISTOTCH/HISTOTCH.html. Accessed on: 7 March 2011.

External links