Difference between revisions of "P63"
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==Classic use== | ==Classic use== | ||
===Positive=== | |||
*Marker of [[squamous cell carcinoma]]. | *Marker of [[squamous cell carcinoma]]. | ||
*[[Urothelial carcinoma]].<ref name=pmid15976812>{{Cite journal | last1 = Lewis | first1 = JS. | last2 = Ritter | first2 = JH. | last3 = El-Mofty | first3 = S. | title = Alternative epithelial markers in sarcomatoid carcinomas of the head and neck, lung, and bladder-p63, MOC-31, and TTF-1. | journal = Mod Pathol | volume = 18 | issue = 11 | pages = 1471-81 | month = Nov | year = 2005 | doi = 10.1038/modpathol.3800451 | PMID = 15976812 | URL = http://www.nature.com/modpathol/journal/v18/n11/full/3800451a.html}}</ref> | *[[Urothelial carcinoma]].<ref name=pmid15976812>{{Cite journal | last1 = Lewis | first1 = JS. | last2 = Ritter | first2 = JH. | last3 = El-Mofty | first3 = S. | title = Alternative epithelial markers in sarcomatoid carcinomas of the head and neck, lung, and bladder-p63, MOC-31, and TTF-1. | journal = Mod Pathol | volume = 18 | issue = 11 | pages = 1471-81 | month = Nov | year = 2005 | doi = 10.1038/modpathol.3800451 | PMID = 15976812 | URL = http://www.nature.com/modpathol/journal/v18/n11/full/3800451a.html}}</ref> | ||
===Invasive versus in situ=== | |||
Thresholding (invasive vs. pre-invasive): | Thresholding (invasive vs. pre-invasive): | ||
*Prostate basal cell marker. | *Prostate basal cell marker. | ||
Revision as of 14:16, 12 May 2016
| P63 | |
|---|---|
| Immunostain in short | |
 p63 staining in squamous epithelium with dysplasia. | |
| Similar stains | p40 (more specific for squamous cell carcinoma) |
| Use | breast pathology (invasion vs. in situ), prostate pathology (HGPIN vs. cancer), lung (adenocarcinoma vs. squamous) |
| Normal staining pattern | nuclear staining |
| Positive | squamous cell carcinoma, urothelial carcinoma |
| Negative | prostate carcinoma, most lung adenocarcinoma, breast carcinoma |
p63 is a commonly used immunostain. p63, like most other "p" stains, is a nuclear stain.
Pattern
- Nuclear staining.
- Stains basal cells in a normal squamous epithelium.
- Superficial cells are typically negative.
- In dysplasia, the staining is seen more superficially,[1] as one might expect as most squamous carcinomas are positive for p63.
Note:
- Cytoplasmic staining suggestive of muscle differentiation - seen in rhabdomyosarcoma.[2]
Classic use
Positive
- Marker of squamous cell carcinoma.
- Urothelial carcinoma.[3]
Invasive versus in situ
Thresholding (invasive vs. pre-invasive):
- Prostate basal cell marker.
- Breast myoepithelial cell marker.
Non-classic tumours
- Di Como et al[4] looked at a large cross-section of tumours.
- Jo and Fletcher[5] did a paper on soft tissue lesions and p63.
See also
References
- ↑ Houghton, O.; McCluggage, WG. (Sep 2009). "The expression and diagnostic utility of p63 in the female genital tract.". Adv Anat Pathol 16 (5): 316-21. doi:10.1097/PAP.0b013e3181b507c6. PMID 19700941.
- ↑ Martin, SE.; Temm, CJ.; Goheen, MP.; Ulbright, TM.; Hattab, EM. (Oct 2011). "Cytoplasmic p63 immunohistochemistry is a useful marker for muscle differentiation: an immunohistochemical and immunoelectron microscopic study.". Mod Pathol 24 (10): 1320-6. doi:10.1038/modpathol.2011.89. PMID 21623385.
- ↑ Lewis, JS.; Ritter, JH.; El-Mofty, S. (Nov 2005). "Alternative epithelial markers in sarcomatoid carcinomas of the head and neck, lung, and bladder-p63, MOC-31, and TTF-1.". Mod Pathol 18 (11): 1471-81. doi:10.1038/modpathol.3800451. PMID 15976812.
- ↑ Di Como, CJ.; Urist, MJ.; Babayan, I.; Drobnjak, M.; Hedvat, CV.; Teruya-Feldstein, J.; Pohar, K.; Hoos, A. et al. (Feb 2002). "p63 expression profiles in human normal and tumor tissues.". Clin Cancer Res 8 (2): 494-501. PMID 11839669.
- ↑ Jo, VY.; Fletcher, CD. (Nov 2011). "p63 immunohistochemical staining is limited in soft tissue tumors.". Am J Clin Pathol 136 (5): 762-6. doi:10.1309/AJCPXNUC7JZSKWEU. PMID 22031315.