Difference between revisions of "Colorectal tumours"
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===Other ancillary studies=== | ===Other ancillary studies=== | ||
*BRAF ''V600E'' missense mutation found in ~10% CRC.<ref name=pmid20635392>{{cite journal |author=Tie J, Gibbs P, Lipton L, ''et al.'' |title=Optimizing targeted therapeutic development: Analysis of a colorectal cancer patient population with the BRAF(V600E) mutation |journal=Int J Cancer |volume= |issue= |pages= |year=2010 |month=July |pmid=20635392 |doi=10.1002/ijc.25555 |url=}}</ref> | *BRAF ''V600E'' missense mutation found in ~10% CRC.<ref name=pmid20635392>{{cite journal |author=Tie J, Gibbs P, Lipton L, ''et al.'' |title=Optimizing targeted therapeutic development: Analysis of a colorectal cancer patient population with the BRAF(V600E) mutation |journal=Int J Cancer |volume= |issue= |pages= |year=2010 |month=July |pmid=20635392 |doi=10.1002/ijc.25555 |url=}}</ref> | ||
*KRAS mutation status. | *[[KRAS mutation]] status. | ||
====BRAF V600E mutation==== | ====BRAF V600E mutation==== | ||
Revision as of 15:21, 6 September 2014
Colorectal tumours, especially colorectal carcinomas, are very common. They are the bread and butter of GI pathology. Non-tumour colon is dealt with in the colon article.
An introduction to gastrointestinal pathology is in the gastrointestinal pathology article. The precursor lesion of colorectal carcinoma (CRC) is, typically, an adenomatous polyp. Polyps are discussed in the intestinal polyps article.
Classification
Most common
- Colon & rectum - most common = adenocarinoma.[1]
Others
Other tumours - many (incomplete list):[2]
- Mucinous carcinoma.
- Need > 50% mucinous component.[3]
- Adenosquamous carcinoma.
- Signet-ring carcinoma.
- Squamous carcinoma.
- Neuroendocrine neoplasms (carcinoid tumours).
- Lipoma.
- Leiomyoma.
- Gastrointestinal stromal tumour (GIST) - dealt with in a separate article.
- Angiosarcoma.
- Lymphoma (Non-Hodgkin's lymphoma).
Notes:
- Mucinous carcinoma - percentage required to call varies by site:
Squamous carcinoma
Main article: Squamous carcinoma
- Rare.
- In the context of a rectal tumour, retrograde growth from the anus should be considered.
Staging of colorectal cancer
- "Adenocarcinoma in situ" and "high-grade dysplasia" is used interchangeably by many in the colon and rectum.
- Splitting hairs - adenocarcinoma in situ is invasion into the lamina propria, high-grade dysplasia does not have lamina propria invasion. Ergo, the difference (in my opinion) amounts to seeing a desmoplastic stroma (adenocarcinoma) or not seeing one (dysplasia).
Staging of tumours:
- Tis - in situ (intramucosal).
- T1 - into submucosa (through mucularis mucosae).
- This is different than elsewhere, e.g. in the small bowel tumour cells in the lamina propria is defined as T1. The rationale for the T1 definition in CRC is that no lymphatics are present in the mucosa, ergo no risk of distant spread.
- T2 - into muscularis propria.
- T3 - into fat beyond musclaris propria.
- T4 - into something else.
Nodes:
- N0 - no positive nodes.
- N1 - 1-3 positive nodes.
- N2 - 4+ positive nodes.
Notes:
- Memory device for N2 = 4+ +ve LNs: the number four in chinese sounds like death.[4]
Overall stage
Simple version
Tumour/node grade for stage:[5]
- Stage I - T1 or T2 N0 M0.
- Stage II - T3 or T4 N0 M0.
- Stage III - Tx N1 or N2 M0.
- Stage IV - Tx Nx M1.
Complex version
Detailed tumour/node grade for stage:[6]
- Stage I - T1 or T2.
- Stage IIA - T3.
- Stage IIB - T4.
- Stage IIIA - T1 N1 or T2 N1.
- Stage IIIB - T3 N1 or T4 N1.
- Stage IIIC - Tx N2.
- Stage IV - Tx Nx M1.
Pathogenesis of colorectal carcinoma
Overview
Colorectal carcinoma is thought to arise from one of two pathways:[7][8]
- APC (adenomatous polyposis coli) gene mutation pathway, AKA classic adenoma-carcinoma pathway.
- Serrated pathway, AKA mutator pathway, mismatch repair pathway.
Syndromes
Both of the above described pathways are associated with syndromes:
- Familial adenomatous polyposis (FAP) or familial polyposis coli (FPC).
- Lynch syndrome (AKA hereditary non-polyposis colorectal cancer syndrome (HNPCC)).
Pathways
APC gene mutation pathway
Microscopic:
Mismatch repair pathway
- Associated with microsatellite instability (MSI).
Other ancillary studies
- BRAF V600E missense mutation found in ~10% CRC.[9]
- KRAS mutation status.
BRAF V600E mutation
Features:[9]
- Independently associated with BRAF V600E:
- Usually older (>70 years old).
- Female gender.
- Right-sided tumour location.
- Worse prognosis - in the context of metastatic disease.
KRAS mutation
Main article: KRAS mutation
- Patient must have wild type KRAS to get drugs; KRAS mutation predicts resistance to cetuximab (Erbitux) and panitumumab (Vectibix).
- Cetuximab and panitumumab are EGFR inhibitors.
Microsatellite instability cancers
- Abbreviated MSI cancers.
General
- Can be sporadic, i.e. non-syndromic.[12]
- Strong association with Lynch syndrome.[13][12]
Features:[14]
- Prognosis: slightly better than other CRC without MSI.
- Treatment implication: different response to chemotherapy.
MSI classification
MSI associated cancers can be classified into:[15][16]
- MSI-H >= 30% of loci have abnormality.
- MSI-L <30% of loci have abnormality.
Note:
- In the context of no chemotherapy, individuals with MSI-H tumours have a superior outcome to those with MSI-L tumours.[17]
- With chemotherapy the outcomes are similar.
Gross
Features:[14]
- Location: proximal colon, i.e. right-sided, predominance.
Microscopic
Features:[14]
- Lymphocytic infiltrate - see intratumoural lymphocytic response.
- Pushing border.[18]
- Histomorphology:
- Poorly differentiated.
- Mucinous.
- Signet ring.
- Medullary.[19]
Molecular
Commonly associated abnormalities in the genes:
- MLH1.
- PMS2.
- MSH2.
- MSH6.
Less common abnormalities:
- PMS1.
- MLH3.
- MSH3.
IHC
Immunostains are commonly done for:
- MLH1.
- PMS2.
- MSH2.
- MSH6.
IHC interpretation:
- Loss of nuclear staining in nuclei of the tumour implies a mutation.
- Nuclear staining = normal.
- Patchy MSH6 is considered normal.[citation needed]
MSI staining loss patterns:[20]
- MLH1 and PMS2 are often lost together, as MLH1 loss results in PMS2 loss.
- MSH2 and MSH6 are often lost together, as MSH2 loss results in MSH6 loss.
Implication of MSI staining loss patterns:
- PMS2 & MSH6 can be used as a screen.[20]
Etiology/significance loss of staining
- MSH2 mutations (IHC stain -ve) - often associated with a germline mutation,[21] while mutations in MLH1 are usually sporatic.[22]
- PMS2 mutations (IHC stain -ve) - often associated with a germline mutation.[23]
How to remember the more important MSI stuff:
- The MSHs are paired together.
- MSH (Mount Sinai Hospital) is where they started it in the city.
- PMS sucks... it's with the other one (MLH).
- The higher numbers in the pairings (PMS2, MSH6) are the screening tests (High Screen Pass).
- The 2s (MSH2, PMS2) are associated with germline mutations (Four legs good two legs bad!).
Specific entities
Colorectal adenocarcinoma
Main article: Colorectal adenocarcinoma
See also
- Anus - covers anal cancer and anal intraepithelial neoplasia.
- Colon.
- Gastrointestinal pathology.
- Tumour budding.
References
- ↑ Cotran, Ramzi S.; Kumar, Vinay; Fausto, Nelson; Nelso Fausto; Robbins, Stanley L.; Abbas, Abul K. (2005). Robbins and Cotran pathologic basis of disease (7th ed.). St. Louis, Mo: Elsevier Saunders. pp. 864. ISBN 0-7216-0187-1.
- ↑ Humphrey, Peter A; Dehner, Louis P; Pfeifer, John D (2008). The Washington Manual of Surgical Pathology (1st ed.). Lippincott Williams & Wilkins. pp. 198. ISBN 978-0781765275.
- ↑ Tozawa E, Ajioka Y, Watanabe H, et al. (2007). "Mucin expression, p53 overexpression, and peritumoral lymphocytic infiltration of advanced colorectal carcinoma with mucus component: is mucinous carcinoma a distinct histological entity?". Pathol. Res. Pract. 203 (8): 567–74. doi:10.1016/j.prp.2007.04.013. PMID 17679024.
- ↑ URL: http://en.wikipedia.org/wiki/Numbers_in_Chinese_culture#Four. Accessed on: 28 June 2011.
- ↑ Shiau, Carolyn; Toren, Andrew (2006). Toronto Notes 2006: Comprehensive Medical Reference (Review for MCCQE 1 and USMLE Step 2) (22nd edition (2006) ed.). Toronto Notes for Medical Students, Inc.. pp. GS27. ISBN 978-0968592861.
- ↑ http://www.cancer.org/docroot/CRI/content/CRI_2_4_3X_How_is_colon_and_rectum_cancer_staged.asp
- ↑ Goldstein NS (January 2006). "Serrated pathway and APC (conventional)-type colorectal polyps: molecular-morphologic correlations, genetic pathways, and implications for classification". Am. J. Clin. Pathol. 125 (1): 146–53. PMID 16483003.
- ↑ Rüschoff J, Aust D, Hartmann A (2007). "[Colorectal serrated adenoma: diagnostic criteria and clinical implications]" (in German). Verh Dtsch Ges Pathol 91: 119–25. PMID 18314605.
- ↑ 9.0 9.1 Tie J, Gibbs P, Lipton L, et al. (July 2010). "Optimizing targeted therapeutic development: Analysis of a colorectal cancer patient population with the BRAF(V600E) mutation". Int J Cancer. doi:10.1002/ijc.25555. PMID 20635392.
- ↑ Dunn EF, Iida M, Myers RA, et al. (October 2010). "Dasatinib sensitizes KRAS mutant colorectal tumors to cetuximab". Oncogene. doi:10.1038/onc.2010.430. PMID 20956938.
- ↑ Di Nicolantonio F, Martini M, Molinari F, et al. (December 2008). "Wild-type BRAF is required for response to panitumumab or cetuximab in metastatic colorectal cancer". J. Clin. Oncol. 26 (35): 5705–12. doi:10.1200/JCO.2008.18.0786. PMID 19001320.
- ↑ 12.0 12.1 Heinimann, K. (2013). "Toward a Molecular Classification of Colorectal Cancer: The Role of Microsatellite Instability Status.". Front Oncol 3: 272. doi:10.3389/fonc.2013.00272. PMID 24199172.
- ↑ Mensenkamp, AR.; Vogelaar, IP.; van Zelst-Stams, WA.; Goossens, M.; Ouchene, H.; Hendriks-Cornelissen, SJ.; Kwint, MP.; Hoogerbrugge, N. et al. (Dec 2013). "Somatic mutations in MLH1 and MSH2 are a Frequent Cause of Mismatch-repair Deficiency in Lynch Syndrome-like Tumors.". Gastroenterology. doi:10.1053/j.gastro.2013.12.002. PMID 24333619.
- ↑ 14.0 14.1 14.2 Boland CR, Goel A (June 2010). "Microsatellite instability in colorectal cancer". Gastroenterology 138 (6): 2073–2087.e3. doi:10.1053/j.gastro.2009.12.064. PMID 20420947.
- ↑ Lawes DA, Pearson T, Sengupta S, Boulos PB (August 2005). "The role of MLH1, MSH2 and MSH6 in the development of multiple colorectal cancers". Br. J. Cancer 93 (4): 472–7. doi:10.1038/sj.bjc.6602708. PMC 2361590. PMID 16106253. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2361590/.
- ↑ Guidoboni M, Gafà R, Viel A, et al. (July 2001). "Microsatellite instability and high content of activated cytotoxic lymphocytes identify colon cancer patients with a favorable prognosis". Am. J. Pathol. 159 (1): 297–304. PMC 1850401. PMID 11438476. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1850401/.
- ↑ Ribic, CM.; Sargent, DJ.; Moore, MJ.; Thibodeau, SN.; French, AJ.; Goldberg, RM.; Hamilton, SR.; Laurent-Puig, P. et al. (Jul 2003). "Tumor microsatellite-instability status as a predictor of benefit from fluorouracil-based adjuvant chemotherapy for colon cancer.". N Engl J Med 349 (3): 247-57. doi:10.1056/NEJMoa022289. PMID 12867608.
- ↑ Pollet, A. 18 October 2010.
- ↑ Truta B, Chen YY, Blanco AM, et al. (2008). "Tumor histology helps to identify Lynch syndrome among colorectal cancer patients". Fam. Cancer 7 (3): 267–74. doi:10.1007/s10689-008-9186-8. PMID 18283560.
- ↑ 20.0 20.1 Hall, G.; Clarkson, A.; Shi, A.; Langford, E.; Leung, H.; Eckstein, RP.; Gill, AJ. (2010). "Immunohistochemistry for PMS2 and MSH6 alone can replace a four antibody panel for mismatch repair deficiency screening in colorectal adenocarcinoma.". Pathology 42 (5): 409-13. doi:10.3109/00313025.2010.493871. PMID 20632815.
- ↑ Mangold E, Pagenstecher C, Friedl W, et al. (December 2005). "Tumours from MSH2 mutation carriers show loss of MSH2 expression but many tumours from MLH1 mutation carriers exhibit weak positive MLH1 staining". J. Pathol. 207 (4): 385–95. doi:10.1002/path.1858. PMID 16216036.
- ↑ A. Pollett. 2010.
- ↑ Vaughn CP, Robles J, Swensen JJ, et al. (May 2010). "Clinical analysis of PMS2: mutation detection and avoidance of pseudogenes". Hum. Mutat. 31 (5): 588–93. doi:10.1002/humu.21230. PMID 20205264.