Latest revision as of 15:29, 4 February 2014

This article is a brief collection things electron microscopy. It is commonly abbreviated EM.

Fixation for EM is often done with glutaraldehyde.^[1]

Ultrastructural findings

Glycogen

General

Seen in:

Ultrastructure

Appearance:

Cytoplasmic, electron dense, not bound by a membrane.

Tubuloreticular inclusions

Abbreviated TRIs.

General

Seen in:

Ultrastructure

Appearance:

Undulating tubules in the smooth ER, usu. perinuclear.^[2]

Melanosomes

General

Seen in:

Melanoma.

Ultrastructure

Appearance:

Membrane bound, sausage-like shape.

Image(s):

Melanosomes (nature.com).

Neurosecretory granules

AKA dense-core granules.

General

Seen in:

Neuroendocrine carcinoma.
- Merkel cell carcinoma.^[3]

Ultrastructure

Appearance:^[4]

Round membrane bound, granular core.
Size: 100-400 nm.

Image(s):

Neuroendocrine tumours (ucsf.edu).

Birbeck granules

General

Seen in Langerhans cell histiocytosis.

Ultrastructure

Appearance:

Electron dense, cytoplasmic tennis racket-like body.

Image(s):

Birbeck granules (photobucket.com).
- Usually... only the handle of the tennis racket is well seen.

Tonofilaments

General

Found in epithelial cells.
Part of the cytoskeleton.
May be found in the epithelioid component of a biphasic synovial sarcoma.^[5]

Ultrastructure

Features:

Filamentous structures at the cell periphery - vaguely resemble striated muscle.

Image(s):

Tonofilaments (bioscience.org).

Zymogen granules

General

Seen in acinic cell carcinoma.
Pancreas - acinar cell.^[6]

Ultrastructure

Features:

Round structures ~0.5-1.5 μm.

Images:

Specific diagnoses

Malignant mesothelioma

Main article: Malignant mesothelioma

Several EM features can be useful to distinguish mesothelioma from adenocarcinoma as per Warhol et al:^[8]

Microvillous length -- longer in mesothelioma.
Quantity of intermediate filaments -- mesothelioma have more.

References

↑ Prentø, P. (Nov 1995). "Glutaraldehyde for electron microscopy: a practical investigation of commercial glutaraldehydes and glutaraldehyde-storage conditions.". Histochem J 27 (11): 906-13. PMID 8787969.
↑ Stoltenburg-Didinger G, Genth E (June 2009). "[Dermatomyositis]" (in German). Z Rheumatol 68 (4): 287–94. doi:10.1007/s00393-008-0398-y. PMID 19330338.
↑ Gil-Moreno, A.; Garcia-Jiménez, A.; González-Bosquet, J.; Esteller, M.; Castellví-Vives, J.; Martínez Palones, JM.; Xercavins, J. (Mar 1997). "Merkel cell carcinoma of the vulva.". Gynecol Oncol 64 (3): 526-32. PMID 9062165.
↑ URL: http://www.pathologyimagesinc.com/emhandbook/diagn-organelles-section/organelle-pages/neuroendo-granules.html. Accessed on: 11 February 2011.
↑ Fisher, C. (Dec 1998). "Synovial sarcoma.". Ann Diagn Pathol 2 (6): 401-21. PMID 9930576.
↑ Gorelick, FS.; Otani, T. (Jul 1999). "Mechanisms of intracellular zymogen activation.". Baillieres Best Pract Res Clin Gastroenterol 13 (2): 227-40. PMID 11030603.
↑ URL: http://www3.mpibpc.mpg.de/groups/em/Gallery.htm. Accessed on: 1 June 2012.
↑ Warhol, MJ.; Hickey, WF.; Corson, JM. (Jun 1982). "Malignant mesothelioma: ultrastructural distinction from adenocarcinoma.". Am J Surg Pathol 6 (4): 307-14. PMID 6287872.

External links

Electron microscopy atlas (uni-mainz.de).

[pmid8787969-1] Prentø, P. (Nov 1995). "Glutaraldehyde for electron microscopy: a practical investigation of commercial glutaraldehydes and glutaraldehyde-storage conditions.". Histochem J 27 (11): 906-13. PMID 8787969.

[pmid19330338-2] Stoltenburg-Didinger G, Genth E (June 2009). "[Dermatomyositis]" (in German). Z Rheumatol 68 (4): 287–94. doi:10.1007/s00393-008-0398-y. PMID 19330338.

[pmid9062165-3] Gil-Moreno, A.; Garcia-Jiménez, A.; González-Bosquet, J.; Esteller, M.; Castellví-Vives, J.; Martínez Palones, JM.; Xercavins, J. (Mar 1997). "Merkel cell carcinoma of the vulva.". Gynecol Oncol 64 (3): 526-32. PMID 9062165.

[4] URL: http://www.pathologyimagesinc.com/emhandbook/diagn-organelles-section/organelle-pages/neuroendo-granules.html. Accessed on: 11 February 2011.

[pmid9930576-5] Fisher, C. (Dec 1998). "Synovial sarcoma.". Ann Diagn Pathol 2 (6): 401-21. PMID 9930576.

[pmid11030603-6] Gorelick, FS.; Otani, T. (Jul 1999). "Mechanisms of intracellular zymogen activation.". Baillieres Best Pract Res Clin Gastroenterol 13 (2): 227-40. PMID 11030603.

[7] URL: http://www3.mpibpc.mpg.de/groups/em/Gallery.htm. Accessed on: 1 June 2012.

[pmid6287872-8] Warhol, MJ.; Hickey, WF.; Corson, JM. (Jun 1982). "Malignant mesothelioma: ultrastructural distinction from adenocarcinoma.". Am J Surg Pathol 6 (4): 307-14. PMID 6287872.

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[4]

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[8]

@@ Line 1: / Line 1: @@
-This article collection stuffs about '''electron microscopy'''.
+This article is a brief collection things '''electron microscopy'''.  It is commonly abbreviated '''EM'''.
+[[Fixation]] for EM is often done with glutaraldehyde.<ref name=pmid8787969>{{Cite journal  | last1 = Prentø | first1 = P. | title = Glutaraldehyde for electron microscopy: a practical investigation of commercial glutaraldehydes and glutaraldehyde-storage conditions. | journal = Histochem J | volume = 27 | issue = 11 | pages = 906-13 | month = Nov | year = 1995 | doi =  | PMID = 8787969 }}</ref>
+=Ultrastructural findings=
 ==Glycogen==
+===General===
+Seen in:
+*[[Glycogen storage disorders]].
+*[[Ewing sarcoma]].
+===Ultrastructure===
 Appearance:
 *Cytoplasmic, electron dense, not bound by a membrane.
-Seen in:
-*[[Glycogen storage disorders]].
 ==Tubuloreticular inclusions==
 Abbreviated ''TRIs''.
+===General===
+Seen in:
+*[[Dermatomyositis]].
+*[[Inclusion body myositis]].
+===Ultrastructure===
 Appearance:
 *Undulating tubules in the smooth ER, usu. perinuclear.<ref name=pmid19330338>{{cite journal |author=Stoltenburg-Didinger G, Genth E |title=[Dermatomyositis] |language=German |journal=Z Rheumatol |volume=68 |issue=4 |pages=287–94 |year=2009 |month=June |pmid=19330338 |doi=10.1007/s00393-008-0398-y |url=}}</ref>
+==Melanosomes==
+===General===
 Seen in:
-*[[Dermatomyositis]].
+*[[Melanoma]].
-*Inclusion body myositis.
-==Melanosomes==
+===Ultrastructure===
 Appearance:
 *Membrane bound, sausage-like shape.
@@ Line 25: / Line 37: @@
 *[http://www.nature.com/nrm/journal/v8/n10/fig_tab/nrm2258_F1.html Melanosomes (nature.com)].
+==Neurosecretory granules==
+*[[AKA]] ''dense-core granules''.
+===General===
 Seen in:
-*[[Melanoma]].
+*[[Neuroendocrine carcinoma]].
+**[[Merkel cell carcinoma]].<ref name=pmid9062165>{{Cite journal  | last1 = Gil-Moreno | first1 = A. | last2 = Garcia-Jiménez | first2 = A. | last3 = González-Bosquet | first3 = J. | last4 = Esteller | first4 = M. | last5 = Castellví-Vives | first5 = J. | last6 = Martínez Palones | first6 = JM. | last7 = Xercavins | first7 = J. | title = Merkel cell carcinoma of the vulva. | journal = Gynecol Oncol | volume = 64 | issue = 3 | pages = 526-32 | month = Mar | year = 1997 | doi =  | PMID = 9062165 }}</ref>
+===Ultrastructure===
+Appearance:<ref>URL: [http://www.pathologyimagesinc.com/emhandbook/diagn-organelles-section/organelle-pages/neuroendo-granules.html http://www.pathologyimagesinc.com/emhandbook/diagn-organelles-section/organelle-pages/neuroendo-granules.html]. Accessed on: 11 February 2011.</ref>
+*Round membrane bound, granular core.
+*Size: 100-400 nm.
+Image(s):
+*[http://pathhsw5m54.ucsf.edu/case7/image77.html Neuroendocrine tumours (ucsf.edu)].
+==Birbeck granules==
+===General===
+*Seen in [[Langerhans cell histiocytosis]].
+===Ultrastructure===
+Appearance:
+*Electron dense, cytoplasmic tennis racket-like body.
+Image(s):
+*[http://media.photobucket.com/image/birbeck+granules+/cat_at_uw/Annotated%2520Remembrances/BirbeckGranules.jpg Birbeck granules (photobucket.com)].
+**Usually... only the handle of the tennis racket is well seen.
+==Tonofilaments==
+===General===
+*Found in epithelial cells.
+*Part of the cytoskeleton.
+*May be found in the epithelioid component of a biphasic [[synovial sarcoma]].<ref name=pmid9930576>{{Cite journal  | last1 = Fisher | first1 = C. | title = Synovial sarcoma. | journal = Ann Diagn Pathol | volume = 2 | issue = 6 | pages = 401-21 | month = Dec | year = 1998 | doi =  | PMID = 9930576 }}</ref>
+===Ultrastructure===
+Features:
+*Filamentous structures at the cell periphery - vaguely resemble striated muscle.
+Image(s):
+*[http://www.bioscience.org/atlases/tumpath/em/tonofila.htm Tonofilaments (bioscience.org)].
+==Zymogen granules==
+===General===
+*Seen in [[acinic cell carcinoma]].
+*[[Pancreas]] - acinar cell.<ref name=pmid11030603>{{Cite journal  | last1 = Gorelick | first1 = FS. | last2 = Otani | first2 = T. | title = Mechanisms of intracellular zymogen activation. | journal = Baillieres Best Pract Res Clin Gastroenterol | volume = 13 | issue = 2 | pages = 227-40 | month = Jul | year = 1999 | doi =  | PMID = 11030603 }}
+</ref>
+===Ultrastructure===
+Features:
+*Round structures ~0.5-1.5 μm.
+Images:
+*[http://db2.photoresearchers.com/preview/9C3411.html Zymogen granules (photoresearchers.com)].
+*[http://www3.mpibpc.mpg.de/groups/em/Gallery/Pnacreas/158wt1.jpg Zymogen granules (mpg.de)].<ref>URL: [http://www3.mpibpc.mpg.de/groups/em/Gallery.htm http://www3.mpibpc.mpg.de/groups/em/Gallery.htm]. Accessed on: 1 June 2012.</ref>
+=Specific diagnoses=
+==Malignant mesothelioma==
+{{Main|Malignant mesothelioma}}
+Several EM features can be useful to distinguish mesothelioma from adenocarcinoma as per Warhol ''et al'':<ref name=pmid6287872>{{Cite journal  | last1 = Warhol | first1 = MJ. | last2 = Hickey | first2 = WF. | last3 = Corson | first3 = JM. | title = Malignant mesothelioma: ultrastructural distinction from adenocarcinoma. | journal = Am J Surg Pathol | volume = 6 | issue = 4 | pages = 307-14 | month = Jun | year = 1982 | doi =  | PMID = 6287872 }}</ref>
+*Microvillous length -- longer in mesothelioma.
+*Quantity of intermediate filaments -- mesothelioma have more.
-==See also==
+=See also=
 *[[Microscope]].
 *[[Medical kidney diseases]].
-==References==
+=References=
-{{reflist}}
+{{reflist|2}}
-==External links==
+=External links=
 *[http://www.uni-mainz.de/FB/Medizin/Anatomie/workshop/EM/EMGlykogenE.html Electron microscopy atlas (uni-mainz.de)].
 [[Category:Weird stuff]]

Difference between revisions of "Electron microscopy"

Latest revision as of 15:29, 4 February 2014

Contents

Ultrastructural findings

Glycogen

General

Ultrastructure

Tubuloreticular inclusions

General

Ultrastructure

Melanosomes

General

Ultrastructure

Neurosecretory granules

General

Ultrastructure

Birbeck granules

General

Ultrastructure

Tonofilaments

General

Ultrastructure

Zymogen granules

General

Ultrastructure

Specific diagnoses

Malignant mesothelioma

See also

References

External links

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