48,466
edits
Difference between revisions of "Tissue fixation"
Jump to navigation
Jump to search
more
m (→External links: +overview of histol.) |
(more) |
||
| Line 4: | Line 4: | ||
Pathologist have a great lifestyle 'cause tissue takes long to fix; the penetration of tissue by formalin is 1 mm/hour.<ref>Gross rounds. 14 August 2009.</ref> | Pathologist have a great lifestyle 'cause tissue takes long to fix; the penetration of tissue by formalin is 1 mm/hour.<ref>Gross rounds. 14 August 2009.</ref> | ||
== | ==Ratio== | ||
The dictum is:<ref>URL: [http://www.cap.org/apps/docs/committees/cancer/cancer_protocols/2011/UvealMelanom_11protocol.pdf http://www.cap.org/apps/docs/committees/cancer/cancer_protocols/2011/UvealMelanom_11protocol.pdf]. Accessed on: 27 March 2012.</ref> | |||
*''The volume of fixative should be 10x the volume of specimen.'' | |||
* | |||
==Tissue fixation== | ==Tissue fixation== | ||
| Line 40: | Line 39: | ||
*Formaldehyde + methanol. | *Formaldehyde + methanol. | ||
= | =Fixing marking dye= | ||
To fix marking dye: | |||
*Formal-acetic alcohol (FAA):<ref>URL: [http://www.brunelmicroscopes.co.uk/acatalog/Solvents___Reagents.html http://www.brunelmicroscopes.co.uk/acatalog/Solvents___Reagents.html]. Accessed on: 5 January 2011.</ref> | |||
*Bouin's solution. | |||
==Formal-acetic-alcohol== | ==Formal-acetic-alcohol== | ||
General: | General: | ||