Talk:Urothelium

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Revision as of 11:10, 25 October 2013 by Michael (talk | contribs) (→‎Ki-67: new section)
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Dysplasia

URINARY BLADDER LESION, DOME, BIOPSY:
- UROTHELIAL DYSPLASIA, SEE COMMENT.
- BENIGN MUSCULARIS PROPRIA.

COMMENT:
The urothelium's nuclei have mild atypia, as would be seen in a low-grade papillary
urothelial carcinoma. 

Immunostaining is as follows:
p53 - weak to moderate staining of 10% of the urothelium, predominantly basal.
CK20 - focal staining of surface cells (umbrella cells), otherwise negative.
Ki-67 - 20-30% of urothelium, predominantly basal.

Ki-67

  • 60x objective... estimated ~21 cells across. Thus, ~350 cells in the field (21*21/4*pi ~ 346).
    • If it is 20 cells... it is 314 cells in the field.
  • Field Diameter = 22 mm/60 = 0.366667 mm.
  • Average cell size = 367 micrometres/21 ~= 17 micrometres.