Difference between revisions of "Papanicolaou stain"

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'''Papanicolaou stain''', abbreviated '''Pap stain''', is a standard [[stain]] used in [[cytopathology]].  It is a modified [[H&E stain]].
'''Papanicolaou stain''', abbreviated '''Pap stain''', is a standard [[stain]] used in [[cytopathology]].<ref>{{Cite journal  | last1 = Prabhudesai | first1 = NM. | last2 = Kulkarni | first2 = MB. | last3 = Desai | first3 = SB. | last4 = Borges | first4 = AM. | title = Modified H & E staining technique for fine needle aspiration cytology (FNAC) smears. | journal = Indian J Pathol Microbiol | volume = 47 | issue = 3 | pages = 384-6 | month = Jul | year = 2004 | doi =  | PMID = 16295430 }}</ref> It is a modified [[H&E stain]].


==General==
==General==
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==See also==
==See also==
*[[Stains]].
*[[Stains]].
==References==
{{Reflist|1}}


[[Category:Stains]]
[[Category:Stains]]

Revision as of 04:12, 24 April 2016

Papanicolaou stain
Stain in short

Low grade squamous intraepithelial lesion.
Abbreviation Pap stain
Similar stains Romanowsky stains
Use the standard stain in cytopathology
Interpretation blue/purple = nucleus, pink/green = cytoplasm, orange = keratin

Papanicolaou stain, abbreviated Pap stain, is a standard stain used in cytopathology.[1] It is a modified H&E stain.

General

  • Can be thought of as the H&E of cytopathology.
  • Specimens are fixed in ethanol.
  • Good for seeing nuclear detail.
  • Out-of-focus cytoplasm is translucent; allows one to focus overlapped cells in different planes.

Use

  • Cytopathology.

Interpretation

  • Blue/purple = nucleus.
  • Green/pink = cytoplasm.
  • Orange = keratin.

Images

See also

References

  1. Prabhudesai, NM.; Kulkarni, MB.; Desai, SB.; Borges, AM. (Jul 2004). "Modified H & E staining technique for fine needle aspiration cytology (FNAC) smears.". Indian J Pathol Microbiol 47 (3): 384-6. PMID 16295430.