Difference between revisions of "Papanicolaou stain"

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{{ Infobox stain
| Name      = {{PAGENAME}}
| Image      = Low grade squamous intraepithelial lesion.jpg
| Width      =
| Caption    = Low grade squamous intraepithelial lesion.
| Abbrev    = Pap stain
| Synonyms  =
| Variants  =     
| Similar    = [[Romanowsky stain]]s     
| Use        = the standard stain in [[cytopathology]]
| Subspecial = 
| Interpret  = blue/purple = nucleus, pink/green = cytoplasm, orange = keratin
| Positive  =
| Negative  =
| Other      =
}}
'''Papanicolaou stain''', abbreviated '''Pap stain''', is a standard [[stain]] used in [[cytopathology]].  It is a modified [[H&E stain]].
'''Papanicolaou stain''', abbreviated '''Pap stain''', is a standard [[stain]] used in [[cytopathology]].  It is a modified [[H&E stain]].



Revision as of 04:10, 24 April 2016

Papanicolaou stain
Stain in short

Low grade squamous intraepithelial lesion.
Abbreviation Pap stain
Similar stains Romanowsky stains
Use the standard stain in cytopathology
Interpretation blue/purple = nucleus, pink/green = cytoplasm, orange = keratin

Papanicolaou stain, abbreviated Pap stain, is a standard stain used in cytopathology. It is a modified H&E stain.

General

  • Can be thought of as the H&E of cytopathology.
  • Specimens are fixed in ethanol.
  • Good for seeing nuclear detail.
  • Out-of-focus cytoplasm is translucent; allows one to focus overlapped cells in different planes.

Use

  • Cytopathology.

Interpretation

  • Blue/purple = nucleus.
  • Green/pink = cytoplasm.
  • Orange = keratin.

Images

See also