Difference between revisions of "Papanicolaou stain"

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*Orange = keratin.
*Orange = keratin.


===Image===
===Images===
<gallery>
<gallery>
Image:Urine_citology_urothelial_carcinoma_2.jpg | Pap stain - [[urine cytology]] (WC)
Image:Low grade squamous intraepithelial lesion.jpg|LSIL - Pap stain. (WC)
Image:High-grade squamous intraepithelial lesion.jpg|HSIL - Pap stain. (WC)
</gallery>
<gallery>
Image:Urine_citology_urothelial_carcinoma_2.jpg | [[Urine cytology]] - Pap stain. (WC)
</gallery>
</gallery>



Revision as of 04:08, 24 April 2016

Papanicolaou stain, abbreviated Pap stain, is a standard stain used in cytopathology. It is a modified H&E stain.

General

  • Can be thought of as the H&E of cytopathology.
  • Specimens are fixed in ethanol.
  • Good for seeing nuclear detail.
  • Out-of-focus cytoplasm is translucent; allows one to focus overlapped cells in different planes.

Use

  • Cytopathology.

Interpretation

  • Blue/purple = nucleus.
  • Green/pink = cytoplasm.
  • Orange = keratin.

Images

See also