Difference between revisions of "Papanicolaou stain"

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#redirect [[Stains#Papanicolaou_stain]]
'''Papanicolaou stain''', abbreviated '''Pap stain''', is a standard [[stain]] used in [[cytopathology]].  It is a modified [[H&E stain]].
 
==General==
*Can be thought of as the [[H&E stain|H&E]] of [[cytopathology]].
**It is a modified [[H&E stain]].
*Specimens are fixed in ethanol.
*Good for seeing nuclear detail.
*Out-of-focus cytoplasm is translucent; allows one to focus overlapped cells in different planes.
 
==Use==
*Cytopathology.
 
==Interpretation==
*Blue/purple = nucleus.
*Green/pink = cytoplasm.
*Orange = keratin.
 
===Image===
<gallery>
Image:Urine_citology_urothelial_carcinoma_2.jpg | Pap stain - [[urine cytology]] (WC)
</gallery>
 
==See also==
*[[Stains]].


[[Category:Stains]]
[[Category:Stains]]

Revision as of 04:03, 24 April 2016

Papanicolaou stain, abbreviated Pap stain, is a standard stain used in cytopathology. It is a modified H&E stain.

General

  • Can be thought of as the H&E of cytopathology.
  • Specimens are fixed in ethanol.
  • Good for seeing nuclear detail.
  • Out-of-focus cytoplasm is translucent; allows one to focus overlapped cells in different planes.

Use

  • Cytopathology.

Interpretation

  • Blue/purple = nucleus.
  • Green/pink = cytoplasm.
  • Orange = keratin.

Image

See also